Thermo Fisher SPE cartridge equivalent - HyperSep

GL Sciences Equivalent for Thermo Fisher SPE cartridges

Complete list of InertSep equivalent phases for Thermo Fisher SPE columns

HPLC Columns
- InertSustain C18 First choice for ultra-high inertness and high durability, delivering superior peak shapes with low back pressure.
- Inertsil ODS-3 Most popular C18, trusted for long-established methods, offering strong retentivity and high loading capacity.
- InertSustain AQ-C18 First choice for high-polarity compounds, delivering strong retention even under 100% aqueous mobile phases.
- Inertsil ODS-3V Validated version of ODS-3, designed for GLP/GMP compliance, ensuring batch-to-batch consistency.
- InertSustainSwift C18 Ultra-inert, fast-separation C18 for high-throughput LC-MS and LC-MS/MS with superior peak symmetry.
- InertSustain AX-C18 Mixed-mode C18 with anion exchange for strong retention of highly polar acidic compounds without ion-pairing.
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- InertSustain C8 Ultra-inert C8 column for rapid analysis of hydrophobic compounds with symmetric peaks across a wide pH range.
- Inertsil C8 Ultra-pure silica C8 column for rapid analysis of hydrophobic compounds, ideal for legacy methods.
- InertSustainSwift C8 Ultra-inert C8 column for low-polarity analytes, peptides, and oligonucleotides with optimized 200Å pore size.
- Inertsil C8-3 Strong retentivity, very inert, and low back pressure, based on the same silica and bonding as Inertsil ODS-3.
- Inertsil WP 300 Wide-pore C8 column for rapid, high-resolution separation of proteins and peptides with sharper peaks.
- Inertsil C4 Low-retentivity C4 column for rapid analysis of highly hydrophobic compounds like fat-soluble vitamins.
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- InertSustain Amide First choice for HILIC mode, offering the strongest retention of polar compounds with superior stability in water-rich conditions.
- Inertsil HILIC Diol-bonded HILIC column for excellent peak shape and strong retention of highly polar basic compounds.
- InertSustain NH2 First choice for sugar analysis, offering superior stability, reproducibility, and compatibility with weakly acidic eluents.
- Inertsil NH2 Aminopropyl column for sugar analysis, highly retentive in normal phase with superior stability and reproducibility.
- ProteoSil HILIC Bioseparation-focused HILIC column, excelling in hydrophilic compound, peptide, glycan, and oligonucleotide analysis.
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- InertSustain PFP A pentafluorophenyl-modified column designed for enhanced separation of structurally similar compounds through multiple retention mechanisms, including π-π, dipole, and hydrogen bonding interactions.
- InertSustain Phenyl A directly bonded phenyl group gives the InertSustain Phenyl column unique reversed-phase selectivity, ideal for separating polar compounds and structural isomers via π-π and hydrogen bonding interactions.
- InertSustain Phenylhexyl A phenylhexyl-bonded column that offers complementary selectivity to traditional alkyl-chain columns, providing high inertness, reproducibility, and low back pressure.
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Sample Preparation
- InertSep C18 Silica sorbent with high end-capping, ideal for lipid removal & pesticide residue analysis.
- InertSep C18-ENV C18 sorbent with low end-capping, optimized for water analysis & anionic surfactant removal.
- InertSep HLB Versatile SPE column for extracting non-polar to highly polar compounds, with a wide pH range (1-14).
- InertSep C8 Silica sorbent with octyl groups, offering weaker retention than C18 for highly retained compounds.
- InertSep SCX Silica-based SPE column with strong cation exchange and non-polar interactions for enhanced retention.
- InertSep SAX Monofunctional C18 sorbent with medium end-capping, allowing secondary interactions for versatile use.
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- MonoSpin C18 High-permeability spin column for peptide desalting, drug extraction, and fast biological sample preparation.
- MonoSpin ProA Fast Protein A spin column for high-purity IgG antibody purification with >90% recovery.
- MonoSpin C18-CX Mixed-mode spin column with C18 and cation exchange for enhanced basic drug recovery.
- MonoSpin Phospholipid TiO₂/ZrO₂-coated spin column for efficient phospholipid removal and matrix effect reduction.
- MonoSpin SAX Strong anion exchange spin column for fast extraction of acidic drugs with high recovery.
- MonoSpin TiO TiO₂ spin column for phospholipid removal and organophosphorus pesticide purification.
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- InertSep QuEChERS Kit High-purity extraction and cleanup kit for pesticide residue analysis in food and environmental samples.
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- SPE Vacuum Manifolds
- SPE Gravity Manifolds
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GC Columns
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- InertCap Series
- TC Series
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- InertCap 1MS General purpose, Hydrocarbons, PCBs, High Volatile solvents, Phenols
- InertCap 1 General purpose, Hydrocarbons, PCBs, High Volatile solvents, Phenols
- InertCap 5MS/Sil General purpose, Halogenated compounds, Phenols, Pesticides, FAME
- InertCap 5MS/NP General purpose, Halogenated compounds, Phenols, Pesticides, FAME
- InertCap 5 General purpose, Halogenated compounds, Phenols, Pesticides, FAME
- InertCap 624MS Residual solvents of Pharmaceuticals, VOCs, Alcohols
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- TC-70
- TC-2560
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Instruments
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- LD249 Gas Leak Detector Compact, easy-to-use leak detector with thermal conductivity sensor for fast, accurate gas detection. Replaces the LD239.
- FM4: Air Sampler for PFAS Low-volume air sampler for PFAS enabling comprehensive analysis of both gas and particle phases in a single sampling using stage-optimized materials.
- Intelligent Pump UI-22 Series Low-pulsation linear motor pump with precise flow control and bubble-free operation.
- OralChroma Halitosis Measuring Device High-precision breath analyzer using gas chromatography to quantify VSCs in 4 minutes — fast, reliable, and maintenance-free.
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- LD249 Gas Leak Detector Latest model replacing LD239, with thermal conductivity detection for helium, hydrogen, and other gases.
- GF1010 Digital Gas Flowmeter Portable, volumetric meter for accurate GC gas flow measurement without gas selection.
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- Intelligent Pump UI-22 Series Low-pulsation linear motor pump with precise flow control and bubble-free operation.
- Dual Pump KP-21 Series High-precision, medium to high-pressure pump with RS-232C control and customizable seals.
- Auto BPR BP-11 Auto Back Pressure Regulator for the UI-22 Series, maintaining stable pressure for consistent flow.
- PCS Pump SP-22 Series Single plunger pump with a pressure sensor, pulse control, and real-time pressure display for precise monitoring.
- Dual Pump KP-22 Series High-precision, low-pulsation pump with pulse control, real-time pressure display, and RS-232C control.
- PCS Pump SP-21 Series Cost-effective single plunger pump with pulse control, ideal for basic solvent dispensing.
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- Gastorr AG Series Reliable, CPU-controlled degassing system for low-flow applications with independent chamber design.
- Gastorr BG Series High-performance degassing system with real-time vacuum display and independent chamber design.
- Gastorr FG-42 Solvent degassing system optimized for fluorinated solvents with real-time vacuum display.
- Gastorr PG Series High-flow degassing system for fractionating applications with real-time vacuum display.
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- PFAS Solutions Products and workflows for EPA and ISO-compliant PFAS testing in water, air, and soil.
- Flow Chemistry Solutions Integrated pumps and pressure regulators for continuous, high-temperature flow chemistry reactions.
- Life Science/Bio Products Spin columns and purification tools for antibody capture, protein cleanup, and bioseparation workflows.
- Gas/Air Sampling Equipment Sampling tools for capturing VOCs, aldehydes, and trace gases in environmental and industrial settings.
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InertSep SPE columns from GL Sciences are designed for easy and cost-effective preparation of laboratory samples. With a wide variety of polymer and silica-based sorbents, InertSep SPE columns allows you to prepare samples with the exact separation mode and chemistry needed.

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Available in multiple formats:

Cartridge	Large-size cartridge	Luer Device Type	96-Well Plate

For effective extraction of a wide variety of compounds from non-polar to high-polarity using a hydrophilic-lipophilic balanced phase

InertSep HLB

InertSep HLB is a composite polymer solid phase of N-containing vinyl polymer and styrene divinylbenzene. By optimizing the balance between hydrophobicity and hydrophilicity with a unique synthetic technology, InertSep HLB can retain and elute compounds with a wide range of polarities.

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Available in several particle sizes for different flow requirements.

High-efficiency reversed phase extraction with a highly end-capped C18 phase

InertSep C18

Recommended GL Sciences equivalent for Thermo Fisher HyperSep C18

InertSep C18 is a silica-based sorbent modified with C18 for non-polar interactions. With our high-level end-capping technology, cation exchange by interaction with the residual silanol groups is suppressed, which reduces adsorption of basic compounds. This sorbent is suitable for removing lipid in simultaneous analysis of pesticide residues in agricultural products.

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Available in several particle sizes for different flow requirements.

	InertSep C18	InertSep C18 FF
Average particle diameter (μm)	60	120
Carbon Load (%)	19
Surface area (m²/g)	450
Pore volume (mL/g)	0.7
Fine pore size (nm)	6
pH range of use	2-8

2x4 Method Phases

GL Sciences provides several mixed-mode SPE sorbents for use with the 2x4 Method. GL Sciences SPE phases feature stable holding power, excellent permeability and selection of particle sizes including large diameters with high flow-rate ideal for sample pre-treatment.

	InertSep MCX FF	InertSep MAX FF	InertSep WCX FF	InertSep WAX FF
Recommended equivalent to	HyperSep Retain CX	HyperSep Retain AX
Functional group	―SO₃^-	―N^-(CH₃)₃	―COO^-	―NR₂
	Learn More	Learn More	Learn More	Learn More
Average particle diameter (μm)	70
Surface area (m²/g)	480
Pore volume (mL/g)	1.1
Fine pore size (nm)	9
pH range of use	1-14
Ion exchange capacity (meq/g)	1.2	0.3	1.2	0.7

InertSep HLB

InertSep HLB FF

Average particle diameter (μm)

Surface area (m²/g)

Pore volume (mL/g)

Fine pore size (nm)

pH range of use

Thermo Fisher	Base Gel	Mode	InertSep
	Polymer based	Reversed Phase	InertSep HLB
			InertSep PLS-3
			InertSep Pharma
HyperSep Retain PEP			EZ Cartridge RP-1
			InertSep PLS-2
HyperSep Retain CX		Ion Exchange	InertSep MCX
			InertSep WCX
HyperSep Retain AX			InertSep MAX
			InertSep WAX
HyperSep C18	Silica based	Reversed Phase	InertSep C18
			InertSep C18-B
			InertSep C18-C
HyperSep C8			InertSep C8
			InertSep C2
			InertSep CH
HyperSep Phenyl			InertSep PH
HyperSep Cyano		Normal Phase	InertSep CN
HyperSep Diol			InertSep 2OH
HyperSep Silica			InertSep SI
HyperSep Carboxylic acid(WCX)		Ion Exchange	InertSep CBA
			InertSep PRS
HyperSep SCX			InertSep SCX
			InertSep SCX-2
HyperSep Aminopropyl (WAX)			InertSep NH2
			InertSep PSA
HyperSep SAX			InertSep SAX
			InertSep SAX-2
		Polar	InertSep AL-A
			InertSep AL-N
			InertSep AL-B
HyperSep Florisil			InertSep FL
			InertSep FL-PR
	Others	Special	InertSep AC
HyperSep Hypercarb			InertSep GC
			InertSep DRY
HyperSep SLE			InertSep K-solute
			InertSep GC/PSA
			InertSep GC/NH2
			InertSep GC/SAX/PSA
			InertSep SAX/PSA
			InertSep DNPH
HyperSep SLE			Phospholipid Remover
			FastRemover MF (0.2um filter)
Pierce Protein Precipitaion plate			FastRemover for Protein
HyperSep SLE Plates			FastRemover for Phospholipid
			InertSep PCB

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