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Ion-Exchange HPLC Columns – High-Performance Anion and Cation Exchange Chromatography

GL Sciences Ion-Exchange HPLC Columns deliver precise, reproducible separation of charged analytes through advanced anion- and cation-exchange chemistry.

Designed for scientists seeking reliability and clarity in HPLC ion-exchange chromatography, these columns provide exceptional resolution, stable retention, and batch-to-batch consistency across pH 2 to 7.5.

Whether for pharmaceutical QC, biochemical research, or method development, GL Sciences columns ensure accurate, high-efficiency performance in every run.

Ion Exchange HPLC Columns
 
 

Recommended HPLC Ion Exchange Columns

 

Inertsil AX – Anion-Exchange HPLC Column

Engineered with a diethylamino functional group on high-purity silica, Inertsil AX provides stable, predictable retention for acidic and negatively charged analytes. It delivers high-resolution separation of nucleotides, organic acids, and phosphorylated compounds, with excellent reproducibility and durability across pH 2–7.5.

Ideal for laboratories performing anion-exchange HPLC of acidic or polar molecules.

Key Specifications
USP Code:―
Functional Group:Diethylamino
Surface Area:450 m²/g
Particle Size:5 µm
Pore Size:100 Å (10 nm)
Carbon Loading:17%
End-Capping:None
pH Range:2 - 7.5
Select Column Specifications

Inertsil CX – Cation Exchange HPLC Column

Featuring a benzenesulfonyl functional group, Inertsil CX ensures strong cation-exchange capacity and precise selectivity for amines, amino acids, and basic drugs. The column maintains sharp peak shapes and consistent retention under varying buffer conditions, making it reliable for both HPLC and UHPLC systems.

Designed for cation-exchange HPLC of basic analytes in pharmaceutical and biochemical workflows.

Key Specifications
USP Code:L14
Functional Group:Benzenesulfonyl
Surface Area:450 m²/g
Particle Size:5 µm
Pore Size:100 Å (10 nm)
Carbon Loading:14%
End-Capping:None
pH Range:2 - 7.5
Select Column Specifications
 
 
 

What Is Ion-Exchange HPLC Chromatography?

Ion-exchange HPLC separates analytes according to their ionic charge interactions with functional groups immobilized on the stationary phase. GL Sciences Inertsil AX and Inertsil CX columns utilize high-purity silica chemically bonded with ion-exchange functional groups that enable the selective retention of acidic or basic analytes.

 
  • Inertsil AX: anion-exchange column with a diethylamino functional group for acidic, negatively charged compounds (e.g., nucleotides, organic acids).
Comparison of Lot-to-lot Reproducibility with Other Brands

Figure 1: Comparison of Lot-to-lot Reproducibility with Other Brands

  • Inertsil CX: cation-exchange column with a benzenesulfonyl group for basic, positively charged compounds (e.g., amines, amino acids).
Comparison of Ion Exchange Capacity with Other Brands

Figure 2: Comparison of Ion Exchange Capacity with Other Brands

Retention can be finely tuned by adjusting mobile phase pH, ionic strength, and buffer type, allowing method developers to achieve optimal resolution even for structurally similar analytes.

 

How Ion-Exchange Works

The ion-exchange separation process follows four key stages:

 

1. Equilibration

Column equilibrated with buffer at controlled pH and ionic strength.

2. Sample Loading

Charged analytes interact with oppositely charged functional groups.

3. Elution

Changing the salt concentration or pH releases analytes in order of charge strength.

4. Regeneration

Column is washed and re-equilibrated for the next analysis.

This mechanism provides reproducible, high-efficiency separations without sacrificing column lifetime or system compatibility.

 

Advantages of GL Sciences Ion-Exchange Columns

 
  • High selectivity and peak symmetry for ionic compounds
  • Stable retention and long column life (pH range 2–7.5)
  • Excellent reproducibility between lots and runs
  • Compatible with HPLC and UHPLC systems
  • Suitable for pharmaceutical QC, biochemical analysis, and metabolite profiling
Comparison of Inertsil AX and CX Columns

Figure 3: Comparison of Inertsil AX and CX Columns

 
 
 

Technical Resources & Support

 

Ion Exchange Column Selection Guide

Technical guide featuring Inertsil AX and CX ion exchange columns with application examples, specifications, and performance data.

Download PDF Guide →

Application Notes

Real-world method examples for pharmaceutical, biochemical, environmental, and food analysis applications using ion exchange chromatography.

Browse Application Library →
 
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