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SPE Columns / Cartridges

Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more

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SKU# 5010-62705
$338.00
SKU# 5010-27541
$454.00
SKU# 5010-27540
$180.00
SKU# 5010-66465
$270.00
SKU# 5010-66470
$236.00
SKU# 5010-65805
$454.00
SKU# 5010-66492
$320.00
SKU# 5010-27418
$338.00
SKU# 5010-27525
$136.00
SKU# 5010-62725
$338.00
SKU# 5010-65825
$454.00
SKU# 5010-66491
$236.00
SKU# 5010-27537
$640.00
SKU# 5010-27331
$390.00
SKU# 5010-66450
$270.00
SKU# 5010-66461
$236.00
SKU# 5010-66460
$236.00
SKU# 5010-66480
$236.00
SKU# 5010-62765
$338.00
SKU# 5010-66482
$320.00
SKU# 5010-65835
$454.00
SKU# 5010-27408
$338.00
SKU# 5010-66490
$236.00
SKU# 5010-65815
$454.00
SKU# 5010-27538
$842.00
SKU# 5010-62706
$488.00
SKU# 5010-66466
$270.00
SKU# 5010-27539
$136.00
SKU# 5010-66442
$270.00
SKU# 5010-66471
$236.00
SKU# 5010-65795
$454.00
SKU# 5010-66481
$236.00
SKU# 5010-66462
$320.00
SKU# 5010-66486
$270.00
SKU# 5010-27490
InertSep Trial Kit-1 is 10 sets of reversed phase RP-1 (250 mg/6 mL), PLS-2 (265 mg/6 mL), and PLS-3 (200 mg/6 mL). It is useful for screening organic compounds and regulated pollutants in environmental water in Japan. It is also useful for extracting antimicrobial agents that remain in meat and organs because it is highly permeable to li...
$330.00
SKU# 5010-27491
InertSep Trial Kit-2 is a set of reversed phase Pharma, RP-C18, and MPC (60 mg/3 mL each). It is a convenient combination for pretreatment of medical screening using LC/MS and GC/MS. It is optimum for studying extraction procedures in simultaneous analysis of target compounds and their metabolites.
$260.00
SKU# 5010-27492
InertSep Trial Kit-3 is a set of four ion-exchange systems, InertSep MA 1, MA-2, MC-1, and MC-2 (100 mg/3 mL each). This is a convenient combination for screening ionic compounds. Two types of cationic and anionic products with different ionic strengths are prepared. Since the InertSep MC and MA series are methacrylate-based and have extr...
$320.00
SKU# 5010-27494
InertSep Trial Kit-5 consists of 10 sets of InertSep GC (500 mg/6 mL), InertSep GC/NH2 (500/500 mg/6 mL) and InertSep GC/SAX/PSA (500/500/500 mg/20 mL) as dye removal clean-up kits for the pretreatment of pesticide residue analysis of crops.
$430.00
SKU# 5010-27534
InertSep HLB is a reversed phase sorbent made of styrene-divinylbenzene (SDB) and a nitrogen-containing vinyl monomer.
$330.00
SKU# 5010-68111
InertSep AL-N/C18/SAX/PSA is a four-layer-type solid phase extraction cartridge, from the top layer it packed with AL-N (100 mg), C18 (200 mg), and SAX (100 mg) and PSA (100 mg). The cartridge is small-packed volume and small reservoir size, suitable for rapid analysis. The main application field is simultaneous screening analysis of pest...
$440.00
SKU# 5010-27536
InertSep HLB is a reversed phase sorbent made of styrene-divinylbenzene (SDB) and a nitrogen-containing vinyl monomer.
$240.00
SKU# 5010-27305
InertSep MA-1 is a methacrylate polymer based sorbent modified with strong anion exchange functional groups. The primary advantages of this sorbent are high hydrophilicity and attainability of easy and effective elution of isolates.
$450.00
SKU# 5010-27524
InertSep HLB is a reversed phase sorbent made of styrene-divinylbenzene (SDB) and a nitrogen-containing vinyl monomer.
$330.00
SKU# 5010-27535
InertSep HLB is a reversed phase sorbent made of styrene-divinylbenzene (SDB) and a nitrogen-containing vinyl monomer.
$220.00
SKU# 5010-23010
$340.00
SKU# 5010-27493
InertSep Trial Kit-4 is 10 sets of InertSep SI, InertSep FL, InertSep AL N 1 g/6 mL as a normal phase clean-up kit.
$230.00
InertSep HLB is a solid-phase extraction column that can effectively extract compounds with a wide range of physical properties from non-polar to high polar. Specifications Average Particle Size: 30/60 µm Surface Area: 720 m2/g Pore Volume: 1.3 mL/g Pore Size: 7 nm pH Range: 1 - 14
From $136.00
InertSep MAX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for recovery from samples containing basic and neutral impurities. Specifications Average Particle Size: 70 µm Surface A...
From $160.00
InertSep MCX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong cation-exchange group. Its reversed-phase, cation-exchange action and exceptional retention of basic compounds make it suitable for recovery from samples containing acidic and neutral impurities. Specifications Average Particle Size: 70 µm Surface...
From $160.00
InertSep WAX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for purification of samples containing basic and neutral impurities. By controlling the pH, it can be used to recover acidic ...
From $160.00

Available Formats for SPE Columns

InertSep SPE Columns are available in the following formats:

 

Cartridge

Cartridge format
  • Cartridge volume: 1, 3, 6, 12, 20, 60, 150 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Processing of small liquid samples up to bulk

96-well plate

 
96-well plate format
  • Cartridge volume: 1, 2 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Rapid processing for multiple samples
 

Luer Device type

Slim and Slim-J Luer device format

Slim & Slim-J type​

  • Dimensions: Slim: 8.8 mm O.D. length 32 mm, 21mm; Slim-J: 8.8 mm O.D. length 51 mm, 31 mm
  • Cartridge material: PP housing and PE frit 
  • Purpose: Tandem processing and automatic SPE system 
InertSep Luer mini format

Mini type

  • Dimensions: 12.7 mm O.D. length 32 mm, 21 mm 
  • Cartridge material: PP housing and PE frit 
  • Purpose: manual processing, tandem processing and automatic SPE system
 

Concept of Solid Phase Extraction (SPE)

Main approaches of SPE

The principle of SPE is divided into the following two methods:

1. Retention of Target Analyte

 
Retention of Target Analyte in SPE

2. Retention of Sample Matrix

 
Retention of Sample Matrix in SPE
 

General Steps of SPE

General Steps of SPE
 

General Methods for Sample Processing

Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems. 

General Methods for Sample Processing in SPE
 

Available Sorbents for SPE Columns

InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available. 

 

Polymer-based Sorbents

The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.

 

Silica-based Sorbents

Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica. 

 

Specialty Phases

 

Retentive Capacity of a Sorbent Compared to Sorbent Mass

Retentive Capacity of SPE Sorbent compared to sorbent mass

Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.

 
 

How to Select a Sorbent

 

One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.

How to select an SPE sorbent

Recommendation for Selecting an Ion Exchange Sorbent

 
Differences in selectivity between SPE phases
 

In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.

Recommendation for Selecting an Ion Exchange Sorbent

 

InertSep Quality Control

InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.

 

SPE Columns / Cartridges Resources:​

  • Download our entire 2022 Consumables Catalog Download
  • Search Application Notes For SPE Columns / Cartridges — Search Applications Database
  • SPE Column / Cartridge selection guide — Download the guide to select the right sorbent and column / cartridge Download
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