Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more
The principle of SPE is divided into the following two methods:
1. Retention of Target Analyte
2. Retention of Sample Matrix
Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems.
InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available.
The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.
Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica.
Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.
One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.
In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.
InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.