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SPE Columns / Cartridges

Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more

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InertSep HLB is a solid-phase extraction column that can effectively extract compounds with a wide range of physical properties from non-polar to high polar. Specifications Average Particle Size: 30/60 µm Surface Area: 720 m2/g Pore Volume: 1.3 mL/g Pore Size: 7 nm pH Range: 1 - 14
From $140.00
InertSep MAX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for recovery from samples containing basic and neutral impurities. Specifications Average Particle Size: 70 µm Surface A...
From $144.00
InertSep MCX FF is a sorbent based on styrene divinylbenzene polymer modified by a strong cation-exchange group. Its reversed-phase, cation-exchange action and exceptional retention of basic compounds make it suitable for recovery from samples containing acidic and neutral impurities. Specifications Average Particle Size: 70 µm Surface...
From $144.00
InertSep WAX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for purification of samples containing basic and neutral impurities. By controlling the pH, it can be used to recover acidic ...
From $144.00
InertSep WCX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak cation-exchange group. Its reversed-phase, cation-exchange action make it ideal for pretreatment of basic compounds such as MCX. By controlling the pH, it can be used to recover strongly basic compounds under acidic conditions. Specifications Average P...
From $144.00
InertSep C18 is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. With our high-level end-capping technology, cationic interaction with the silanol groups on the gel is virtually eliminated, which reduces adsorption of basic compounds. This sorbent is suitable for removing lip...
From $151.00
InertSep C18-B is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. In addition to the interaction, secondary interaction can be expected on this mono functional, general purpose sorbent. Specifications Average Particle Size: 45 µm Carbon Loading: 14 % End-capping: Middle ...
From $220.00
InertSep C18 FF is an arranged version of InertSep C18 for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 120 µm Carbon Loading: 19 % End-capping: High Surface Area: 450 m2/g Pore Volume: 0.7 mL/g ...
From $163.00
InertSep C18-C is a silica-based sorbent modified with C18 (octadecyl) functional groups being utilized for non-polar interaction. In addition to the general purpose non-polar interaction, secondary interaction between unbonded silanol groups on silica substrate and isolates can be expected on this trifunctional C18. Specifications Aver...
From $163.00
InertSep C18 ENV is a C18 solid phase synthesized for water analysis. It is useful for sample treatment of anion surfactants in water. Specifications Average Particle Size: 60 µm Carbon Loading: 16 % End-Capping:Low-end capped Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm pH Range: 2 - 8
From $168.00
InertSep C18-C FF is an arranged version of InertSep C18-C for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 120 µm Carbon Loading: 16 % End-Capping: Low Surface Area: 450 m2/g Pore Volume: 0.7 mL/...
From $182.00
InertSep C8 is a silica-based sorbent modified with C8 (octyl) function groups that offers a weaker non-polar interaction than C18. InertSep C8 is used for isolates that are strongly retained too much on C18. With high-level end-capping, cationic interaction with the silanol groups on silica substrate is virtually eliminated and adsorptio...
From $1.00
InertSep C2 is a silica-based sorbent modified with C2 (ethyl) functional groups being utilized for a weaker non-polar interaction than C8. InertSep C2 is used for isolates that are strongly retained too much on C8. As end-capping is accomplished on this sorbent, cation exchange interaction with none bonded silanol groups is virtually eli...
From $66.00
InertSep CH is a silica-based sorbent modified with cyclohexyl functional groups that gives this sorbent a similar moderate polarity with InertSep C2. InertSep CH offers a unique selectivity for the extraction of certain chemical compounds, compared with C18, C8, C2 and PH. Specifications Average Particle Size: 60 μm Carbon Load: 7.5 %...
From $237.00
InertSep PH is a silica-based sorbent modified with phenyl functional groups having a similar non-polar interaction to C8. Phenyl functional group has π-π bond interaction which allows an isolate molecule bearing benzene ring, such as aromatic compounds to selectively interact with phenyl functional group on the sorbent. Specifications ...
From $1.00
InertSep PLS-2 is a SDB (Styrene divinylbenzene) polymer based reversed phase mode sorbent. Compared to a silica based C18 sorbent, InertSep PLS-2 has a quite higher sorbent retentive capacity and better stability in pH range. Specifications Average Particle Size: 70 µm Surface Area: 700 m2/g Pore Volume: 1.1 mL/g Pore Size: 7 nm pH Range...
From $252.00
InertSep PLS-3 is a copolymer based sorbent comprised of nitrogen-containing methacrylate and SDB, it exhibits a wide enough range of retention capability from highly polar to hydrophobic compounds. Specifications Average Particle Size: 60 µm Surface Area: 600 m2/g Pore Volume: 1.1 mL/g Pore Size: 7 nm pH Range: 1 - 14
From $214.00
InertSep RP-1 is a copolymer based sorbent comprised of methacrylate and SDB, it exhibits a wide enough range of retention capability from low-polar to hydrophobic compounds. It is also suit for clean up. Specifications Average Particle Size: 70 µm Surface Area: 650 m2/g Pore Volume: 1.5 mL/g Pore Size: 9 nm pH Range: 1 - 14 *1: mini Type
From $287.00
InertSep RP-2 is a SDB polymer based sorbent modified with weak anion exchange and hydrogen bonding functional groups. This sorbent is suit for concentration of low-retentive polar compounds with RP-1 and simultaneous screening under polar interactions. Specifications Average Particle Size: 90 µm Surface Area: 700 m2/g Pore Volume: 0.7 mL...
From $344.00
InertSep C18-B FF is an arranged version of InertSep C18-B for applications that need high flow late. This sorbent is also good at viscous biological sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 120 µm Carbon Loading: 14 % End-Capping:Middle Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Po...
From $220.00
InertSep RP-C18 is a SDB polymer based sorbent modified with alkyl chain. Specifications Average Particle Size: 45 µm Surface Area: 110 m2/g Pore Volume: 0.5 mL/g Pore Size: 18 nm pH Range: 1 - 13 Remark: Dichloromethane is not available
From $258.00
InertSep MPC is a SDB polymer based sorbent modified with strong cation exchange and C18 functional groups. Because of its hydrophobic and ion-exchange properties, it is useful for pretreatment of basic compounds from hydrophobic compounds in blood and urine for simultaneous analysis of metabolites. It can also be used for separation and ...
From $241.00
InertSep Pharma is a copolymer based sorbent comprised of nitrogen-containing methacrylate and SDB. This sorbent was developed for simultaneous screening of drug metabolites in biological sample. Specifications Average Particle Size: 30 µm Surface Area: 600 m2/g Pore Volume: 1.1 mL/g Pore Size: 7 nm pH Range: 1 - 14
From $279.00
InertSep Pharma FF is an arranged version of InertSep Pharma for applications that need high flow rate and good at viscous biological sample and large volume sample. Specifications Average Particle Size: 60 µm Surface Area: 600 m2/g Pore Volume: 1.1 mL/g Pore Size: 7 nm pH Range: 1 - 14
From $300.00
InertSep MA-1 is a methacrylate polymer based sorbent modified with strong anion exchange functional groups. The primary advantages of this sorbent are high hydrophilicity and attainability of easy and effective elution of isolates. Specifications Average Particle Size: 70 µm Surface Area: 250 m2/g Pore Volume: 0.7 mL/g Pore Size: 13...
From $298.00
InertSep MA-2 is a methacrylate polymer based sorbent modified with, which includes a weak anion exchange function group. Because polymer base gel occurs little secondary interaction, SPE by genuine anion exchange interaction can be achieved with this sorbent. Specifications Average Particle Size: 70 µm Surface Area: 250 m2/g Pore Volume:...
From $259.00
InertSep MC-1 is a methacrylate polymer based sorbent modified with strong cation exchange functional groups. The primary advantages of this sorbent are high hydrophilicity and attainability of easy and effective isolation of cationic compounds. Specifications Average Particle Size: 70 µm Surface Area: 80 m2 Pore Volume: 0.4 mL/g Pore Siz...
From $298.00
InertSep MC-2 is a methacrylate polymer based sorbent modified with week cation exchange functional groups. This sorbent is suitable for SPE of strong anion compounds in ion exchange mode. Because polymer base gel occurs little secondary interaction, SPE by genuine cation exchange interaction can be achieved with this sorbent. Specificati...
From $298.00
InertSep ME-1 is a methacrylate copolymer based solid sorbent modified with iminodiacetic acid, weak cation exchange functional groups. It is highly hydrophilic and does not retain monovalent Na ion or K ion, but it does retain metal divalent or more cations. This offers selective concentration of such metal ions and is suitable for custo...
From $357.00
InertSep ME-2 is a chelating resin sorbent, developed for SPE of trace metal ions in seawater. As this sorbent does not retain Ca and Mg ions, desalting can be achieved by passing the sample through the sorbent and wash with purified water. Specifications Average Particle Size: 70 µm Surface Area: 80 m2/g Pore Volume: 0.5 mL/g Pore Size: ...
From $363.00
InertSep CN is a silica-based sorbent modified with cyanopropyl functional groups. Having both non-polar and polar interactions, InertSep CN is ideal for applications in which extremely non-polar isolates would irreversibly retain on more non-polar sorbents such as C18 or C8 and very polar isolates might be retained irreversibly on the mo...
From $260.00
InertSep 2OH is a silica-based sorbent modified with diol functional groups. Being a fairly polar sorbent, InertSep 2OH is typically used for polar extractions from low-polar or non-polar solvents. Specifications Average Particle Size: 60 μm Carbon Load: 10 % Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm pH Range: 2 ...
From $168.00
InertSep SI is a bare silica, which has strong polar interaction. It offers selective separation for similar compounds in structure using low-polar solvents. InertSep SI is regarded as the most polar sorbent available. Care should be taken with humidity and polar solvents, when using this material. Specifications Average Particle Size: ...
From $147.00
InertSep SI FF is an arranged version of InertSep SI for applications that need high flow late. This sorbent is also good at viscous sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 110 μm - 125 μm Surface Area: 400 m2/g - 550 m2/g Pore Volume: 0.60 mL/g - 0.80 mL/g Pore Size: 5.5 nm - 7...
From $136.00
InertSep AL-A is packed with alumina (Al2O3). Available in Al2O3 acidic format. Specifications Average Particle Size: 100 µm Surface Area: 130 m2/g Pore Volume: 0.3 mL/g Pore Size: 8 nm pH Range: 3.5 - 5.0(acid)
From $184.00
InertSep AL-B is packed with alumina (Al2O3). Available in Al2O3 basic format. Specifications Average Particle Size: 100 μm Surface Area: 130 m2/g Pore Volume: 0.3 mL/g Pore Size: 8 nm pH: 9.0 - 10.5(basic)
From $184.00
InertSep AL-N is packed with alumina (Al2O3). Available in Al2O3 neutral format. Specifications Average Particle Size: 100 μm Surface Area: 130 m2/g Pore Volume: 0.3 mL/g Pore Size: 8 nm pH Range: 6.0 - 7.5 (neutral)
From $182.00
Florisil SPE cartridges are packed with magnesium silica, highly polar material which strongly adsorb polar compounds from none polar matrices and typically used for sample cleanup of organic extracts. Specifications Average Particle Size: 50 - 200 µm Surface Area: 230 m2/g Pore Volume: 0.5 mL/g Pore Size: 9 nm
From $161.00
InertSep FL-PR cartridges are packed with FL-PR, which is used for sample cleanup for analysis of residual pesticides in crops. This sorbent is also good at viscous sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 100 - 300 μm Surface Area: 230 m2/g Pore Volume: 0.5 mL/g Pore Size: 9 nm
From $159.00
InertSep CBA is a silica-based sorbent modified with carboxyl ethyl functional groups. The primary interactions of this sorbent are cation exchange and secondary interactions are week-polar and non-polar. This sorbent is suitable for extraction of drugs with strongly cationic amine groups. Specifications Average Particle Size: 45 μm Ca...
From $207.00
InertSep PRS is a silica-based sorbent modified with propyl-sulfonyl functional groups. The primary interactions of this sorbent are anion exchange and secondary interactions are slightly non-polar. Specifications Average Particle Size: 45 μm Carbon Load: 8.5 % Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm Ion exchan...
From $207.00
InertSep SCX is a silica-based sorbent modified with benzene sulfonyl propyl functional groups. The primary interaction is a combination of both non-polar and strong cation exchange. Because the non-polar interactions on InertSep SCX is stronger than those of InertSep PRS, it is suitable for such an extraction that the combination of non-...
From $203.00
InertSep SCX-2 employs the same chemical modification with InertSep SCX. The only difference is that benzene sulfonyl propyl functional groups are bonded more densely on the silica surface to increase ion exchange capacity and retentivity. Specifications Average Particle Size: 60 μm Carbon Load: 17 % Surface Area: 450 m2/g Pore Volum...
From $163.00
InertSep PCB is a two layer SPE cartridge packed with SCX and SI. It has been designed for the extraction of PCBs from complex matrix. InertSep PCB is generally used for the extraction of isolates from matrices such as oil and milk.
From $273.00
InertSep NH2 is a silica-based sorbent modified with an amino-propyl functional groups. Anion exchange and polar interaction are combined as a primary interactions. As secondary interactions, it has weak non-polar interactions. Similar to InertSep 2OH and InertSep SI used in normal phase mode, InertSep NH2 can be used for the separation o...
From $151.00
InertSep NH2 FF is an arranged version of InertSep NH2 for applications that need high flow late. This sorbent is also good at viscous sample and large volume sample to expedite the procedure. Specifications Average Particle Size: 120 μm Carbon Load: 10 % Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm Ion Exchange Cap...
From $168.00
InertSep PSA is a silica-based sorbent modified with an ethylene-diamine-N-propyl functional groups. The primary interactions of this sorbent are anion exchange and secondary interactions are week non-polar. Specifications Average Particle Size: 60 µm Carbon Load: 11.5 % Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm ...
From $132.00
InertSep SAX is a silica-based sorbent modified with tri-methyl amino propyl functional groups. Primary interactions are very strong anion exchange. Secondary interactions are non-polar exchange. It is generally used for the extraction of weak anions such as carboxylic acid. Specifications Average Particle Size: 45 μm Carbon Load: 7 % ...
From $258.00
InertSep SAX-2 is an arranged version of InertSep SAX for applications that need higher non-polar interactions. Specifications Average Particle Size: 60 μm Carbon Load: 11.5 % Surface Area: 450 m2/g Pore Volume: 0.7 mL/g Pore Size: 6 nm Ion Exchange Capacity: 0.45 meq/g pH Range: 2 - 8 Remark: CI- ion pair
From $180.00
InertSep SAX/PSA is a two layer SPE cartridge packed with SAX and PSA. It is used specifically where the polar interaction is ideal for agrochemical compounds that are often difficult to remove from crop samples.
From $321.00

Available Formats for SPE Columns

InertSep SPE Columns are available in the following formats:

 

Cartridge

Cartridge format
  • Cartridge volume: 1, 3, 6, 12, 20, 60, 150 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Processing of small liquid samples up to bulk

96-well plate

 
96-well plate format
  • Cartridge volume: 1, 2 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Rapid processing for multiple samples
 

Luer Device type

Slim and Slim-J Luer device format

Slim & Slim-J type​

  • Dimensions: Slim: 8.8 mm O.D. length 32 mm, 21mm; Slim-J: 8.8 mm O.D. length 51 mm, 31 mm
  • Cartridge material: PP housing and PE frit 
  • Purpose: Tandem processing and automatic SPE system 
InertSep Luer mini format

Mini type

  • Dimensions: 12.7 mm O.D. length 32 mm, 21 mm 
  • Cartridge material: PP housing and PE frit 
  • Purpose: manual processing, tandem processing and automatic SPE system
 

Concept of Solid Phase Extraction (SPE)

Main approaches of SPE

The principle of SPE is divided into the following two methods:

1. Retention of Target Analyte

 
Retention of Target Analyte in SPE

2. Retention of Sample Matrix

 
Retention of Sample Matrix in SPE
 

General Steps of SPE

General Steps of SPE
 

General Methods for Sample Processing

Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems. 

General Methods for Sample Processing in SPE
 

Available Sorbents for SPE Columns

InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available. 

 

Polymer-based Sorbents

The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.

 

Silica-based Sorbents

Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica. 

 

Specialty Phases

 

Retentive Capacity of a Sorbent Compared to Sorbent Mass

Retentive Capacity of SPE Sorbent compared to sorbent mass

Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.

 
 

How to Select a Sorbent

 

One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.

How to select an SPE sorbent

Recommendation for Selecting an Ion Exchange Sorbent

 
Differences in selectivity between SPE phases
 

In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.

Recommendation for Selecting an Ion Exchange Sorbent

 

InertSep Quality Control

InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.

 

SPE Columns / Cartridges Resources:​

  • Download our entire 2022 Consumables Catalog Download
  • Search Application Notes For SPE Columns / Cartridges — Search Applications Database
  • SPE Column / Cartridge selection guide — Download the guide to select the right sorbent and column / cartridge Download
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