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HPLC Columns

GL Sciences columns provide excellent separation and resolution of target compounds featuring new high-purity evolved surface silica. GL Sciences columns silica base is uniquely modified to provide maximum column performance including low operating back-pressure, high inertness, high efficiency and wide solvent compatibility. Get the appropriate column from a large selection of column configurations and formats compatible with many HPLC methods including analytical, preparative, capillary HPLC and UHPLC. Read more

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For specialized analysis of peptides, nucleic acids, and antibodies. Try ProteoSil now & save 40%! Ends March 31, 2024.
Inertsil ODS-3 is still GL Sciences’ most popular phase and continues to be used widely and reliably for long-established methods in pharmaceutical, and contract research labs. Inertsil ODS-3 columns have a relatively strong retentivity compared to commercially available ODS columns. In addition, the introduction of higher surface area si...
From $280.00
Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. Each Inertsil ODS-3V is delivered with a Manufacturer‘s Validation Certificate showing the detailed results of every QA ...
From $610.00
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise con...
From $280.00
InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 columns to offer significant retention for highly polar comp...
From $280.00
Increasing Efficiency, Throughput and Productivity InertSustainSwift C18 columns are designed to deliver rapid separations with symmetric peaks to minimize analysis time as much as possible. InertSustainSwift C18 is also ideal for LC-MS and LC-MS/MS methods which offer highly sensitive results and enables MS compatible buffers to be u...
From $280.00
InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS.
From $280.00
The High Performance Core-shell Column For UHPLC/HPLC Specifications • Base Material: Core-shell type silica gel • Particle Size: 2.6 µm • Solid Core: 2.0 µm • Surface Area: 200 m2/g • Pore Size: 90 Å (9 nm) • Functional Group: Octadecyl • End-capping: Yes • Carbon Loading: 15% • USP Code: L1 • pH Range: 1 - 10 •...
From $340.00
The 200 Å pore size silica creates the opportunity to separate compounds having a molecular weight from small to large molecules. In addition, the usage of highly inert packing material packed into a new Steel-Coated-PEEK hardware deliver excellent peak shapes for various analytes without any adsorption. Functional Group Base Material...
From $1,050.00
Designed for advanced protein analysis, the ProteoSil 300-C18 column offers the ideal capabilities when enhanced retention and sample load are critical for C4 or C8 columns.
From $192.00
Ideal for mid-sized molecules, the ProteoSil 200-C18 column allows for separation across 100Å pore columns and is easy to clean for faster analysis.
From $192.00
Offering high inertness and durability, the ProteoSil 100-C18 is perfect for analyzing low-molecular-weight compounds, enhancing sensitivity in LC-MS applications.
From $269.00
Ultra High Retentivity Ideal for Separation of Basic Molecules & its Related Substances, Process Impurities Inertsil ODS-HL employs a highly inert packing material which provides pure hydrophobic interaction between analytes without generating any secondary interaction delivering sharp peaks. High-Density bonding of C18 phase delivers ...
From $280.00
Inertsil ODS-4 delivers the same extreme inertness to any type of compounds just like InertSustain C18 along with unprecedented stability under 100 % aqueous mobile phases for qualitative and quantitative analysis. However, as the base silica gel and carbon loading are different on Inertsil ODS-4, differences in selectivity can be observe...
From $280.00
Inertsil ODS-4 columns have proven superior worldwide for analysis of strong pharmaceutical bases, acids, chelating compounds, and zwitterions. The long-awaited validated Inertsil ODS-4V has now been added to our product lineup. Each Inertsil ODS-4V is delivered with a Manufacturer’s Validation Certificate showing the detailed results ...
From $680.00
Inertsil ODS-SP is super base deactivated and optimally bonded to retain hydrophilic compounds without excessive retention of hydrophobic compounds achieving better separations faster than before. As the carbon load of Inertsil ODS-SP is relatively low, it is compatible with 100 % aqueous eluents and offer faster equilibration of column f...
From $280.00
GL Sciences offers a polymerically bonded ODS-P phase which provide high steric selectivity for separation of planar and non-planar compounds. nertsil ODS-P columns are also ideal for the HPLC analysis of 16 PAH compounds, listed as target pollutants by the US EPA.
From $280.00
Inertsil ODS-EP contains a polar functional group embedded between the silica surface and the C18 group. The embedded polar group makes the C18 phase stable in 100 % aqueous eluents without "phase collapse." This phase is also extremely "base deactivated" and provides superior peak shape for acids and bases in organic eluents as well a...
From $280.00
Inertsil WP300 C18 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible separations of proteins and peptides. The results of GL Sciences’ original end-capping technique provide high efficiency and good peak shape for proteins an...
From $280.00
Inertsil ODS-2 columns have a pore size of 150 Å offering symmetric peaks for bases, acids with low pressure. When Inertsil ODS-2 was introduced in 1987s, this HPLC transformed the entire industry. Inertsil ODS-2 was the first HPLC phase created using ultra high purity silica, which produced superior base deactivation. Inertsil ODS-2 is o...
From $280.00
Inertsil ODS columns are general purpose, reversed phase C18 columns available in 5 μm and 10 μm particle sizes. It was the first ODS bonded phase introduced from GL Sciences back in 1986. We recommend InertSustain C18 or Inertsil ODS-4 columns for all new method development.
From $280.00
InertSustain C8 is an octyl group (C8) bonded column delivering the same extreme inertness to any type of compounds just like InertSustain C18, which enables rapid analysis of highly hydrophobic compounds delivering symmetric peaks at a wide range of pH.
From $280.00
InertSustainSwift C8 is an octyl group (C8) bonded column offering the same extreme inertness to any type of compounds just like InertSustainSwift C18, which is ideal for analyzing low polarity analytes. In addition, the optimized 200Å pore size silica enables to analyze and retain peptides and oligonucleotides which have a molecular weig...
From $280.00
Many chromatographers prefer a C8 column when an ODS phase shows excessive retention values. Inertsil C8-4 provides the same separation pattern (selectivity) and extreme inertness to any type of compounds just like Inertsil ODS-4, which enables easy method transfer from ODS-4 to C8-4 while other commercially available ODS and C8 columns c...
From $280.00
The same base silica gel and bonding technology that is used for Inertsil ODS-3 is also employed for Inertsil C8-3. The difference between the two phases is just the length of the hydrocarbon ligands. 10 μm particle size columns are also available to meet the requirement of various pharmacopeia methods. We recommend InertSustain C8 column...
From $280.00
Inertsil C8 columns have a pore size of 150 Å and it is recommended for rapid analysis of highly hydrophobic compounds. We recommend InertSustain C8 or Inertsil C8-4 columns for all new method development.
From $280.00
Optimized for proteins and peptides, the ProteoSil 300-C8 ensures unique selectivity and hydrophobicity compared to shorter-chain C4 phases, suitable for protein and hydrophobic peptide separations.
From $192.00
Specialized for the analysis of mid-sized molecules, the ProteoSil 200-C8 shows superior retention for analytes that are strongly retained by C18 columns
From $304.00
Inertsil C4 columns have a pore size of 150 Å and is recommended for rapid analysis of highly hydrophobic compounds such as fat-soluble vitamins.
From $280.00
Tailored for the analysis of proteins and peptides, especially hydrophobic peptides, the ProteoSil 300-C4 facilitates shorter retention times and is ideal for proteins with moderate retention needs.
From $192.00
Inertsil WP300 C8 (wide pore size of 300 Å) columns bring the same legendary performance of Inertsil's narrow-pore HPLC products to columns designed specifically for the reproducible rapid separations of proteins and peptides. Inertsil WP300 C8 delivers rapid analysis with sharper peaks compared to Inertsil WP300 C18.
From $280.00
Inertsil WP300 C4 is a butyl group bonded phase utilizing silica gel with wide pores (300 Å). Also, an optimal polar group is embedded between silica surface and butyl group, which reduces adsorption of basic compounds and fat-soluble proteins. Inertsil WP300 C4 is recommended for the analysis of large, highly fat-soluble proteins and pep...
From $280.00
InertSustain PFP columns are bonded with pentafluorophenyl groups to our newly developed ES silica gel, which delivers unique separation patterns with excellent peak shape and sensitivity. The chromatographic difference stems from the fact that the PFP phases provide interactions such as π-π, dipole, hydrogen bonding, and ionic interactio...
From $280.00
InertSustain Phenylhexyl columns are bonded with phenylhexyl groups, which employs a phenyl ring with a hexyl (6- carbon) linker and is densely bonded to our newly developed ES silica gel delivering complementary selectivity to straight alkyl-chain columns, but with industry leading inertness, lot-to-lot reproducibility and low back press...
From $280.00
InertSustain Phenyl delivers an extremely unique reverse phase characteristics that are critical to resolving compounds that could not be separated on a C18 or C8 phase. InertSustain Phenyl provides not only pi-pi interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. A...
From $280.00
Just like InertSustain Phenyl, Inertsil Ph-3 has phenyl groups directly bonded to the silica gel which provides pure reverse phase characteristics that are critical to resolving highly polar compounds like acidic and basic pharmaceuticals. The near perfect phenyl phase coverage on this material results in symmetric, narrow peaks for even ...
From $280.00
Inertsil Ph has phenethyl groups bonded to silica gel which offers weak pi-pi interactions. As it is modified with phenethyl groups, hydrophobic interactions between alkyl chain and analytes play an important role in separation as well as π-π interactions. To change the selectivity or elution pattern drastically, InertSustain Phenyl is re...
From $280.00
In general, the stability and reproducibility of the Cyano phase available in the market are poor. Many batch-to-batch or lot-to-lot reproducibility issues are occurring at many laboratories. The InertSustain Cyano columns were developed to resolve these problems and are designed using the most modern LC column technology available provid...
From $280.00
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $280.00
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime.
From $280.00
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $280.00
SKU# HILIC
Modified with amide groups, the ProteoSil HILIC column excels in the separation of highly hydrophilic compounds, peptides, glycans, and oligonucleotides.
From $192.00
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $280.00
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $280.00
Inertsil Diol column has characteristics of dihydroxypropyl group bonded phase. It shows unique selectivity normal phase mode. The separation mechanism of diol column is featured by hydrogen bonding interactions between diol groups and polar compounds. Diol columns provide an alternative selectivity to silica columns often with increased ...
From $280.00
Equipped with dihydroxypropyl groups bonded to silica gel, the ProteoSil 300-SEC column is suited for the analysis of large molecules and can be used as a dial column.
From $192.00
Constructed for aqueous size-exclusion chromatography, the ProteoSil 100-SEC can handle higher operating pressures and is suitable for series column configurations.
From $192.00
Inertsil SIL-100A is a pure silica gel column available in normal phase mode. Because of the high quality of its silica gel, Inertsil SIL-100A achievs separation with sharp peaks and provides high column-to-column reproducibility. This excellent silica gel ideally designed for HPLC is the basis for “Inertsil 3-series” of GL Scineces. GL S...
From $280.00
Inertsil CN-3, Cyanopropryl groups bonded to Silica gel with high density. And it is not only increase difference recognition of hydrophilicity, but also increase the durability. It is difficult to increase the both performance with other previous cyanopropryl columns. Inertsil CN-3 can be cleaned with 100 % aqueous solvent because non-sp...
From $280.00

HPLC Column Types​

GL Sciences offers different column types to support all major separation modes used in HPLC:

Non-polar phases including alkyl phases such as C18, C8, C4 and other functionalities such as phenyl and cyano groups. GL Sciences provides a diverse range of reverse phases for different analyte retentivities and selectivities by group modification. A high degree of analyte targeting can be achieved using our selection of reverse phases.

ColumnFeaturesUSP CodeMicron (µm)Pore Size (nm)Surface Area (m2/g)
InertSustain C18First choice with ultra high inertness and high durabilityL12, 3, 5, 1010350
InertSustain AQ-C18First choice for high polar compoundsL1, L961.9, 3, 510350
InertSustainSwift C18First analysis with ultra high inertness and high durabilityL11.9, 3, 520200
InertSustain AX-C18Analysis of anionic highly polar compoundsL1, L783, 520200
InertCore Plus C18Ideal for analyses requiring a high number of theoretical stagesL12.69200
Inertsil ODS-HLUltra high retentivity, High-density bonding of C18 phaseL13, 510450
Inertsil ODS-4Ultra high inertness, High plate count, Medium retentivityL12, 3, 510450
Inertsil ODS-4VInertsil ODS-4 Validated columnL13, 510450
Inertsil ODS-3Strong retentivity, Lower column backpressure, Very inertL12, 3, 4, 5, 1010450
Inertsil ODS-3VInertsil ODS-3 Validated columnL13, 510450
Inertsil ODS-SPWeak retentivity, for hydrophobic compoundsL13, 510450
Inertsil ODS-PHigh steric selectivityL13, 510450
Inertsil ODS-EPA polar functional group embeddedL1510450
Inertsil WP300 C18Analysis of high moleculesL1530150
Inertsil ODS-2Ultra pure silica gel is usedL1515320
Inertsil ODSInertness 1st generationL15, 1010350
InertSustain C8First choice with ultra high inertness and high durabilityL72, 3, 510350
InertSustainSwift C8High inertness and high durability C8 columnL71.9, 3, 520200
Inertsil C8-4Ultra high inertness, High plate count, Low retentivityL72, 3, 510450
Inertsil C8-3Strong retentivity, Lower column backpressure, Very inertL72, 3, 5, 1010450
Inertsil C8Ultra pure silica gel is usedL7515320
Inertsil C4Low retentivityL26515320
Inertsil WP300 C8Suitable for high moleculesL7530150
Inertsil WP300 C4Suitable for high moleculesL26530150
InertSustain PFPExtremely Strong retention of highly polar basic compoundsL433, 510350
InertSustain PhenylhexylStrong π-π interactions and hydrophobic interactionsL113, 510350
InertSustain PhenylExtremely strong π-π interactionsL112, 3, 510350
Inertsil Ph-3Strong π-π interactionsL112, 3, 510450
Inertsil PhHigh inertness, Weak π-π interactionsL11515320
InertSustain CyanoUltra inertness and can be used in reversed phase modeL103, 510350
 

Phases providing high retentivity of polar compounds in immobilized water layers on the stationary phase surface in hydrophilic interaction liquid chromatography (HILIC) mode. Our choice of funtionalities allow for an excellent spread of different separation patterns for the analysis of compounds such as sugars.

ColumnFeaturesUSP CodeMicron (µm)Pore Size (nm)Surface Area (m2/g)
InertSustain AmideFirst choice for HILIC modeL683, 510350
Inertsil HILICSeparation of highly polar basic compoundsL203, 510450
InertSustain NH2First choice for sugar analysisL83, 510350
Inertsil NH2Sugar analysis, High retentive in normal phase modeL83, 510450
Inertsil AmideIncreasing retentivity of high polar compoundsL683, 510450
 

Phases with ionizable functional groups having high ion exchange capacities are available for both anion and cation exchange chromatography. We feature popular groups for wide method compatibility. In addition, mix-mode columns featuring alkyl groups are available for alternate selectivity.

ColumnFeaturesUSP CodeMicron (µm)Pore Size (nm)Surface Area (m2/g)
Inertsil AXAnion-exchange column510450
Inertsil CXCation-exchange columnL9510450
InertSustain AX-C18Analysis of anionic highly polar compoundsL1, L783, 520200
 

High performance phases for retentivity of polar compounds. Our normal phases are available a wide range of pore sizes (10-30 nm) for excellent choice in retentivity and selectivity in analysis of up to large molecules.

ColumnFeaturesUSP CodeMicron (µm)Pore Size (nm)Surface Area (m2/g)
Inertsil DiolFirst choice for normal phase mode, For SECL203, 510450
Inertsil SIL-100AUltra pure silica gel with 100Å pore sizeL33, 510450
Inertsil SIL-150AUltra pure silica gel with 150Å pore sizeL3515320
Inertsil WP300 SILUltra pure silica gel with 300Å pore sizeL3530150
Inertsil CN-3Can be also used in reversed phase modeL103, 510450
InertSustain CyanoUltra inertness and can be used in reversed phase modeL103, 510350
 

Columns featuring diol phases in convenient pore sizes that are compatible with aqueous (GFC) or organic (GPC) size-exclusion chromatography. Our columns are available in smaller inner diameters for solvent saving, low-cost analysis. Serial coupling using different GL Sciences SEC columns allows for analysis of a broad range of molecular weights. 

ColumnFeaturesUSP CodeMicron (µm)Pore Size (nm)Surface Area (m2/g)
Inertsil WP300 DiolHigh molecule SEC, Can be also used in normal phase modeL20, L33530150
Inertsil DiolFirst choice for normal phase mode, For SECL203, 510450
 

HPLC Column Selection Guide

 

Separation Mode Selection for Analyte Polarity

Separation Mode Selection for Analyte Polarity
 

Column Selection for Analyte Properties

 

Column Selection for Vitamins

Column selection for vitamins
List of vitamins
 

Column Selection for Sugars and Nucleic acids

Column Selection for Sugars and nucleic acids
 

Column Selection for Medicines

Column Selection for Medicines
 

Column Selection for Organic acids

Column Selection for Organic acids
 

Column Selection for Amino acids

Column Selection for Amino acids
 

Column Selection for Proteins and Peptides

Column selection for Proteins and Peptides
 

Column Selection for Pesticides, Veterinary medicines, Aldehydes and Polymers

Column selection for Pesticides, Veterinary Medicines, Aldehydes and Polymers
 

HPLC Columns Resources:​

  • Download GL Sciences 2022 HPLC Columns Catalog — Download
  • Download GL Sciences 2022 InertSustain Catalog— Latest HPLC Column Technology Download
  • Search Application Notes For HPLC Columns — Search Applications Database
  • HPLC Columns’ Specification — Summary of GL Sciences HPLC Column Specifications. Learn More
  • HPLC Column Selection Overview — Separation mode selection for analyte polarity & HPLC column selection for analyte properties. Learn More
  • HPLC Column Selection By USP — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
  • HPLC Column End Fittings — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
  • Reversed Phase HPLC Column Selection Guide— Download Reverse Phase HPLC Column Selection Guide. Learn More
  • Normal Phase Selection Guide — Download Normal Phase HPLC Column Selection Guide. Learn More
  • How to Select a Guard Column — Download guide to select the right guard column. Learn More
  • Choosing a Preparative Column — Download Guide to select the right preparative column. Learn More
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