The following table lists columns that conform to the chromatography column classification set forth by the United States Pharmacopeia (USP) for grouping columns appropriate for reference procedures.
What is a USP column code?
For columns used in high-performance liquid chromatography (HPLC), columns are assigned a USP code starting with L, e.g. L1, L3, L7. The USP L listing groups HPLC columns by characteristics of the stationary phase, however, requirements are broad enough to allow several columns in the same code despite having possibly vastly different performance.
For example, USP code L1 calls for an octadecyl (C18) group bonded to silica or ceramic packing material, 1.5 to 10 µm in diameter, to which many C18 phases of varying carbon load, pore size, and surface area qualify. It is therefore still recommended to consult the actual application a column has been designed for after referring to its USP code as an initial guide.
GL Sciences offers columns for all major USP codes in common use today. GL Sciences InertSustain and Inertsil columns feature high-purity bonded silica phases providing separations with extremely low operating back pressure, high inertness, excellent efficiency and wide compatibility with solvents.
List of HPLC columns by USP code
| USP Code | Description | Brand |
|---|---|---|
USP|L1 | Octadecyl silane chemically bonded to porous or nonporous silica or ceramic microparticles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L2 | Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core, 30 to 50 µm in diameter. | — |
USP|L3 | Porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L4 | Silica gel of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter. | — |
USP|L5 | Alumina of controlled surface porosity bonded to a solid spherical core, 30 to 50 µm in diameter. | — |
USP|L6 | Strong cation exchange packing — sulfonated fluorocarbon polymer coated on a solid spherical core, 30 to 50 µm in diameter. | — |
USP|L7 | Octylsilane chemically bonded to totally porous or superficially porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L8 | An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L9 | Irregular or spherical, totally porous silica gel having a chemically bonded, strongly acidic cation-exchange coating, 3 to 10 µm in diameter. | |
USP|L10 | Nitrile groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L11 | Phenyl groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L12 | A strong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core, 30 to 50 µm in diameter. | — |
USP|L13 | Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diameter. | Inertsil TMS |
USP|L14 | Silica gel having a chemically bonded, strongly basic quaternary ammonium anion-exchange coating, 5 to 10 µm in diameter. | Nucleosil 100-SB Partisil SAX Spherisorb SAX |
USP|L15 | Hexylsilane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter. | Spherisorb C6 |
USP|L16 | Dimethylsilane chemically bonded to porous silica particles, 5 to 10 µm in diameter. | — |
USP|L17 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form, 6 to 12 µm in diameter. | InertSphere FA-1 SUGAR SH1011 SUGAR SH1821 RSpak KC-811 |
USP|L18 | Amino and cyano groups chemically bonded to porous silica particles, 3 to 10 µm in diameter. | Partisil 5 PAC Partisil 10 PAC |
USP|L19 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form, about 9 µm in diameter. | InertSphere Sugar-2 SUGAR SC1011 SUGAR SC1821 SUGAR SC1211 |
USP|L20 | Dihydroxypropane groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter, or a monolithic silica rod. | |
USP|L21 | A rigid, spherical styrene-divinylbenzene copolymer, 3 to 30 µm in diameter. | GPC KF-801 RSpak DS-413 RSpak DS-613 RSpak RP18-415 |
USP|L22 | A cation-exchange resin made of porous polystyrene gel with sulfonic acid groups, about 10 µm in size. | SUGAR SH1011 SUGAR SH1821 RSpak KC-811 SUGAR SP0810 SUGAR SC1011 SUGAR SC1821 SUGAR SZ5532 SUGAR KS800 series |
USP|L23 | An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups, about 7–12 µm in size. | PRP-X500 IEC QA-825 |
USP|L24 | Polyvinylalcohol chemically bonded to porous silica particle, 5 µm in diameter. | — |
USP|L25 | Packing having the capacity to separate compounds with a molecular weight range from 100–5000 (as determined by polyethylene oxide), applied to neutral, anionic, and cationic water-soluble polymers. | OHpak SB-802 HQ OHpak SB-802.5 HQ |
USP|L26 | Butyl silane chemically bonded to totally porous or superficially porous silica particles, 1.5 to 10 µm in diameter. | |
USP|L27 | Porous silica particles, 30 to 50 µm in diameter. | — |
USP|L28 | A multifunctional support consisting of a high purity, 100 Å, spherical silica substrate bonded with anionic exchanger, amine functionality in addition to a conventional reversed phase C8 functionality. | — |
USP|L29 | Gamma alumina, reverse-phase, low carbon percentage by weight, alumina-based polybutadiene spherical particles, 5 µm in diameter with a pore volume of 80 Å. | — |
USP|L30 | Ethyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter. | — |
USP|L31 | A hydroxide-selective, strong anion-exchange resin — quaternary amine bonded on latex particles attached to a core of 8.5 µm macroporous particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. | — |
USP|L32 | A chiral ligand exchange packing — L-proline copper complex covalently bonded to irregularly shaped silica particles, 5 to 10 µm in diameter. | CHIRALPAK WH |
USP|L33 | Packing having the capacity to separate dextrans by molecular size over a range of 4,000 to 500,000 Da. It is spherical, silica-based, and processed to provide pH stability. | Inertsil WP300 Diol PROTEIN KW-800 series |
USP|L34 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form, 7 to 9 µm in diameter. | HC-75(Pb²⁺) SUGAR SP0810 |
USP|L35 | A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type) molecular monolayer bonded phase having a pore size of 150 Å. | — |
USP|L36 | A 3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5 µm aminopropyl silica. | SUMICHIRAL OA-2000 |
USP|L37 | Packing having the capacity to separate proteins by molecular size over a range of 2,000 to 40,000 Da. It is a polymethacrylate gel. | OHpak SB-803 HQ |
USP|L38 | A methacrylate-based size exclusion packing for water-soluble samples. | OHpak SB-800 HQ series |
USP|L39 | A hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin. | OHpak SB-800 HQ series RSpak DM-614 |
USP|L40 | Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles, 5 to 20 µm in diameter. | CHIRALCEL OD series |
USP|L41 | Immobilized α1-acid glycoprotein on spherical silica particles, 5 µm in diameter. | CHIRALPAK AGP |
USP|L42 | Octylsilane and octadecylsilane groups chemically bonded to porous silica particles, 5 µm in diameter. | — |
USP|L43 | Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter. | |
USP|L44 | A multifunctional support consisting of a high purity, 60 Å, spherical silica substrate bonded with a cationic exchanger, sulfonic acid functionality in addition to a conventional reversed phase C8 functionality. | — |
USP|L45 | Beta cyclodextrin, R,S-hydroxypropyl ether derivative, bonded to porous silica particles, 3 to 10 µm in diameter. | ORpak CDBS-453 SUMICHIRAL OA7000 SUMICHIRAL OA7100 |
USP|L46 | Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads, about 9 to 11 µm in diameter. | — |
USP|L47 | High capacity anion-exchange microporous substrate, fully functionalized with trimethylamine groups, 8 µm in diameter. | PRP-X100 PRP-X110 RCX-10 RCX-30 |
USP|L48 | Sulfonated, cross-linked polystyrene with an outer layer of submicron, porous, anion-exchange microbeads, 5 to 15 µm in diameter. | — |
USP|L49 | A reversed phase packing made by coating a thin layer of polybutadiene onto spherical porous zirconia particles, 3 to 10 µm in diameter. | — |
USP|L50 | Multifunction resin with reversed-phase retention and strong anion-exchange functionalities. The resin consists of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm in diameter, and a surface area not less than 350 m²/g. | — |
USP|L51 | Amylose tris-3,5-dimethylphenylcarbamate-coated, porous, spherical, silica particles, 5 to 10 µm in diameter. | CHIRALPAK AD series |
USP|L52 | A strong cation-exchange resin made of porous silica with sulfopropyl groups, 5 to 10 µm in diameter. | — |
USP|L53 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 3 to 15 µm diameter. Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers. | — |
USP|L54 | A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13 µm in diameter. | — |
USP|L55 | A strong cation-exchange resin made of porous silica coated with polybutadiene-maleic acid copolymer, about 5 µm in diameter. | IC-Pak Cation M/D |
USP|L56 | Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter. | — |
USP|L57 | A chiral-recognition protein, ovomucoid, chemically bonded to silica particles, about 5 µm in diameter, with a pore size of 120 Å. | — |
USP|L58 | Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form, about 6 to 30 µm diameter. | SUGAR KS 800 series CXpak P-421S |
USP|L59 | Packing for the size-exclusion separation of proteins over the range of 5 to 7,000 kDa. The packing is a spherical 1.5 to 10 µm silica of hybrid packing with a hydrophilic coating. | PROTEIN KW-803 |
USP|L60 | Spherical, porous silica gel, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and endcapped. | — |
USP|L61 | A hydroxide selective strong anion-exchange resin consisting of a highly cross-linked core of 13 µm microporous particles having a pore size less than 10 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85 nm diameter microbeads bonded with alkanol quaternary ammonium ions (6%). | — |
USP|L62 | C30 silane bonded phase on a fully porous spherical silica, 3 to 15 µm in diameter. | |
USP|L63 | Glycopeptide teicoplanin linked through multiple covalent bonds to a 100-Å spherical silica. | SUGAR KS 800 series |
USP|L64 | Strongly basic anion-exchange resin consisting of 8% cross-linked styrene-divinylbenzene copolymer with a quaternary ammonium group in the chloride form, 45 to 180 µm in diameter. | — |
USP|L65 | Strongly acidic cation-exchange resin consisting of 2% sulfonated cross-linked styrene-divinylbenzene copolymer with a sulfonic acid group in the hydrogen form, 45 to 250 µm in diameter. | — |
USP|L66 | A crown ether coated on a 5 µm particle size silica gel substrate. The active site is (S)-18-crown-6-ether. | Crownpak CR(+) |
USP|L67 | Porous vinyl alcohol copolymer with a C18 alkyl group attached to the hydroxyl group of the polymer, 2 to 10 µm in diameter. | Asahipak ODP-40 Asahipak ODP-50 Shodex ET-RP1 |
USP|L68 | Spherical, porous silica, 10 µm or less in diameter, the surface of which has been covalently modified with alkyl amide groups and not endcapped. | |
USP|L69 | Ethylvinylbenzene/divinylbenzene substrate agglomerated with quaternary amine functionalized 130 nm latex beads, about 6.5 µm in diameter. | — |
USP|L70 | Cellulose tris(phenyl carbamate) coated on 5 µm silica. | CHIRALCEL OC-H SUMICHIRAL OA-3300 |
USP|L71 | A rigid, spherical polymethacrylate, 4 to 6 µm in diameter. | RSpak DE-213 RSpak DE-413 RSpak DE-613 |
USP|L72 | (S)-phenylglycine and 3,5-dinitroanaline urea linkage covalently bonded to silica. | SUMICHIRAL OA-3300 |
USP|L73 | A rigid spherical polydivinylbenzene particle, 5 to 10 µm in diameter. | — |
USP|L74 | A strong anion-exchange resin consisting of a highly cross-linked core of 7 µm macroporous particles having a 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkyl quaternary ammonium ions. | — |
USP|L75 | A chiral-recognition protein, bovine serum albumin (BSA), chemically bonded to silica particles, about 7 µm in diameter, with a pore size of 300 Å. | — |
USP|L76 | Silica based weak cation-exchange material, 5 µm in diameter. Substrate is surface polymerized polybutadiene-maleic acid to provide carboxylic acid functionalities. | — |
USP|L77 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 6 to 9 µm diameter. Substrate is surface grafted with carboxylic acid functionalized groups. | — |
USP|L78 | A silane ligand that consists of both reversed phase (an alkyl chain longer than C8) and anion-exchange (primary, secondary, or tertiary amino groups) functional groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.0 to 50 µm in diameter, or a monolithic rod. | |
USP|L79 | A chiral-recognition protein, human serum albumin (HSA), chemically bonded to silica particles, about 5 µm in diameter. | CHIRALPAK HSA |
USP|L80 | Cellulose tris(4-methylbenzoate)-coated, porous, spherical, silica particles, 5 to 20 µm in diameter. | CHIRALCEL OJ CHIRALCEL OJ-H |
USP|L81 | A hydroxide-selective, strong anion-exchange resin consisting of a highly cross-linked core of 9 µm porous particles having a pore size of 2000 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 70 nm diameter microbeads bonded with alkanol quaternary ammonium ions. | — |
USP|L82 | Polyamine chemically bonded to cross-linked polyvinyl alcohol polymer, 5 µm in diameter. | Asahipak NH2P-40 Asahipak NH2P-50 apHera NH2 Amino |
USP|L83 | A hydroxide-selective, strong anion-exchange resin — quaternary amine bonded on latex particles attached to a core of 10.5 µm microporous particles of 10 Å and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene. | — |
USP|L84 | Weak cation-exchange resin consisting of ethylvinylbenzene, 55% cross-linked with divinylbenzene copolymer, 5 µm in diameter. Substrate is surface grafted with carboxylic acid functionalized groups. | — |
USP|L85 | A silane ligand that consists of both reversed phase (an alkyl chain longer than C8) and weak cation-exchange (carboxyl groups) functional groups chemically bonded to porous or non-porous particles, 1.0 to 50 µm in diameter. | — |
USP|L86 | A 5 µm fused core particle with a highly polar ligand possessing 5 hydroxyl groups tethered to the silica gel outer layer. | — |
USP|L87 | Dodecyl silane chemically bonded to porous silica particles, 1.5 to 10 µm in diameter. | — |
USP|L88 | Glycopeptide vancomycin linked through multiple covalent bonds to 100 Å spherical silica. | — |
USP|L89 | Packing having the capacity to separate compounds with a molecular weight range from 100–3000 (as determined by polyethylene oxide), applied to neutral and anionic water-soluble polymers. | — |
USP|L90 | Amylose tris-[(S)-alpha-methylbenzylcarbamate] coated on porous, spherical silica particles, 3 to 10 µm in diameter. | — |
USP|L91 | Strong anion-exchange resin consisting of monodisperse porous polystyrene/divinyl benzene beads coupled with quaternary amine. Bead size is 3 to 10 µm. | — |
USP|L92 | A strong anion-exchange resin consisting of highly cross-linked 5 to 9 µm macroporous particles having a 100 Å average pore size and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene and an anion-exchange layer grafted to the surface, which is functionalized with alkanol quaternary ammonium ions. | — |
USP|L93 | Cellulose tris(3,5-dimethylphenylcarbamate) reversed phase chiral stationary phase coated on 3 or 5 µm silica gel particles. | CHIRALCEL OD-3R CHIRALCEL OD-RH |
USP|L94 | A strong anion-exchange resin consisting of a highly crosslinked 15 µm microporous particles functionalized with very low crosslinked latex (0.5%) to provide alkanol quaternary ammonium ion exchange sites. | — |
USP|L95 | A highly polar alkyl ligand comprising five hydroxyl groups that are chemically bonded to totally porous or superficially porous silica or a monolithic silica rod. | — |
USP|L96 | Alkyl chain, reversed-phase bonded totally or superficially porous silica designed to retain hydrophilic and other polar compounds when using highly aqueous mobile phases, including 100% aqueous, 1.5 µm to 10 µm. | |
USP|L118 | Aqueous polymerized C18 groups on silica particles, 1.2 to 5 µm in diameter. |
