What is HILIC mode? Hydrophilic Interaction Chromatography Analysis of highly polar and basic compounds has been problematic since the beginning of HPLC. Some polar compounds are so difficult to analyze that they require high aqueous buffer content, even on the most inert reverse phase HPLC columns, resulting in imperfect peak shape and impaired MS sensitivity. Inertsil HILIC has proven particularly effective in detecting melamine in food products.
HILIC was developed as an alternative to reverse phase chromatography for polar compounds, and has also shown great promise in SFC. The elution order in HILIC mode is typically the opposite of what it might be in reverse phase mode. In HILIC, the HIGHER the organic concentration, the GREATER the retention of more polar compounds. Advantages of HILIC mode High organic content mobile phases result in low operating back pressure and ability to run at high flow rates A high organic solvent concentration of the mobile phase will lead to a high sensitivity LC/MS analysis.
Reduced Ion suppression effect in LC/MS analyses. More precise quantitation of polar compounds owing to improved peak shape and the rapid elution of hydrophobic sample components.