InertSustain AX-C18 is a reversed-phase mixed-mode column with an octadecyl group and an alkylamine Anion Exchange group.
It is possible to retain polar acidic components that are generally not retained without the use of ion-pair reagents, and by setting the ion exchange capacity weaker, the buffer mobile phase used in LC / MS can be sufficiently eluted. Is designed for. It also has excellent inter-lot reproducibility, which tends to be a problem with columns.
Chemical bond group silica gel: High-purity spherical silica gel (ES silica)
Particle size: 3µm, 5µm
Surface area: 200m 2 / g
Pore size: 200Å
Pore volume: 1.00 mL / g
Chemical bond group: Octadecyl group + tertiary amino anion exchange group
End-capping: Yes
Carbon Loading: 8%
USP Code: L1, L78
Recommended PH: 1 to 9
You want to slightly change the separation pattern from C18 columns
You want to analyze acidic compounds and anionic compounds
You are having trouble with reproducibility and lot-to-lot variation in anion exchange columns
Conditions:
Column: 3 μm, 150 × 2.1 mm I.D.
Eluent: 25 mM NaH2PO4 in H2O
Flow Rate: 0.2 mL/min
Col. Temp.: 40 ℃
Detection: UV 254 nm
Eluent : 20 mM HCOONH4 in H2O
(Ion pair not used)
Conditions
Column: 3 μm, 150 × 2.1 mm I.D.
Flow Rate: 0.2 mL/min
Col. Temp.: 40 ℃
Sample: 1. UDP‐Glu 2. UDP‐Gal 3. GDP‐Man
Strongly retains highly polar acidic compounds which cannot normally be retained by C18 column for an analysis of highly polar compounds!
Eluent :
A) 10 mM HCOONH4 in CH3OH
B) 10 mM HCOONH4 in H2O
A/B = 5/95 ‐ 10 min ‐ 100/0 ‐ 5 min ‐ 100/0, v/v
Column:3 μm, 150 × 2.1 mm I.D.
Flow Rate:0.2 mL/min
Col. Temp.:40 ℃
Inj. Volume:5 μL
Detection:MS/MS (ESI, Positive, MRM)
Sample: Q1 > Q3
1. CMP: 324.90 > 111.95 (+) 0.5 mg/L
2. GMP: 364.90 > 153.00 (+) 0.5 mg/L
3. UMP: 325.90 > 96.90 (+) 1.0 mg/L
4. AMP: 348.90 > 136.95 (+) 0.05 mg/L
Strongly retains highly polar acidic compounds even when the salt concentration is as low as 10 mM! Sharp elution peaks! Can be analyzed at low salt concentration. Compatibility with MS!
Column : 3 μm, 150 × 4.6 mm I.D. Eluent : CH3CN/H2O = 65/35, v/vFlow Rate : 1.0 mL/minCol. Temp.:40 ℃Detection : UV 254 nm
Sample : 1. Uracil 2. Acetophenone 3. Benzene 4. Toluene 5. Naphthalene
Column : 3 μm, 150 × 4.6 mm I.D. Eluent : CH3CN/0.1 % HCOOH = 10/90, v/v
Flow Rate : 1.0 mL/min
Col. Temp. : 40 ℃
Detection : UV 254 nm
Sample : 1. AMP
Excellent reproducibility of retention time in mixed‐mode columns where reproducibility is difficult to control!
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