InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by AX group works. By controlling the amount of AX group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS.
• Retention behavior that combines both reversed-phase and anion exchange
• Excellent retention for acidic compounds
• Excellent reproducibility and stable quality
Inertsil AX has diethylamino groups bonded to silica gel by an alkyl chain. The diethylamino groups offer anionic functions required for anion exchange chromatography. It is mainly used for analyses of acidic compounds. Conventional ion-exchange columns used to show inconsistent results from lot to lot. However, Inertsil AX is manufactured under strict quality control in order to offer excellent lot-to-lot reproducibility. The retentivity of Inertsil AX is influenced by the buffer concentration in eluent. The retention time can be adjusted by the concentration of buffer.
Inertsil CX has benzenesulfonyl groups bonded to silica gel by an alkyl chain. The sulfony groups at the end of the structure offer cationic functions required for the cation exchange chromatography. It is mainly used for analyses of basic compounds. Inertsil CX is manufactured under strict quality control in order to offer excellent batch to batch reproducibility as the same as Inertsil AX. Inertsil CX has high ion exchange capacity and provides high retentivity and selectivity. Therefore, it is also suited for analyzing amino acids and nucleobases.