InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 to offer significant retention for highly polar compounds even under water rich mobile phases.
• Exceptional retention for highly polar compounds (hydrophilic)
• Highly inert packing material results in less tailing of peaks for virtually any type of analytes
• Extreme resistance to low and high pH mobile phases
• Reliable Reproducibility, performance & quality
• Endlessly reproducible from column-to-column and batch-to-batch
InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by AX group works. By controlling the amount of AX group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS.
• Retention behavior that combines both reversed-phase and anion exchange
• Excellent retention for acidic compounds
• Excellent reproducibility and stable quality
• Chemical bond group silica gel: High-purity spherical silica gel (ES silica)
• Particle size: 3µm, 5µm
• Surface area: 200m 2 / g
• Pore size: 200Å
• Pore volume: 1.00 mL / g
• Chemical bond group: Octadecyl group + tertiary amino group
• End-capping: Yes
• Carbon Loading: 8%
• USP Code: L1, L78
• Recommended PH: 1 to 9
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties.
InertSustain C18 inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide pH analysis with consistent performance from column to column and lot to lot.
• High Inertness
• Extremely Low Operating Back Pressure
• Long Column Lifetime
• pH Range from 1 ～ 10
• Reliable Reproducibility
GL Sciences developed new Silica gel "Evolved Surface Silica” for InertSustain Series which provides both high inertness and high durability. Inert-and-sustainable InertSustain series HPLC columns are the latest models of GL Sciences and are recommended as the first choice for various purpose.
InertSustain Phenyl delivers an extremely unique reverse phase characteristics that are critical to resolving compounds that could not be separated on a C18 or C8 phase. InertSustain Phenyl provides not only pi-pi interactions, but also hydrogen bonding secondary interactions, which results in retaining polar compounds at the same time. As the phenyl groups are directly bonded to the silica gel, InertSustain Phenyl is compatible with the analysis of structural isomers due to it’s high stereo-selectivity while other alkyl phenyl type columns fails to separate.
• Newly developed evolved surface silica gel
• Superior peak shape with better resolution
• Wide pH compatibility and high durability
• Strict quality control program results in high reproducibility
• Unmatched operating back pressure
InertSustain features Unmatched Inertness & Durability
Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases.
InertSustain employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide pH analysis with consistent performance from column to column and lot to lot.
Radically New Silica Gel
1st Stage [Synthesis of “Evolved Surface Silica” - It is not possible to end-cap 100 % of residual silanols using traditional chemical modification procedures. GL Sciences studied the possibility of developing a radically new type of silica, a silica that would provide both high inertness (base deactivation) and durability at a wide range of pH.
2nd Stage [Chemical Bonding of ODS group] - The introduction of Evolved Surface Silica and our cutting-edge chemical bonding technology make InertSustain compatible with 100 % aqueous mobile phases, while maintaining strong non-polar retentivity
3rd Stage [Complete End-capping] - GL Sciences’ complete end-capping technology allows InertSustain to provide high efficiency and superior peak shape even to those well-known strong adsorptive analytes.
Developing a new method is a time-consuming operation. On top of this selecting an HPLC column plays a major role to achieve the desired separation for your critical analysis. The InertSustain series are offered with C18, C8, Phenyl and Aminopropyl phases, which cover an extended range of selectivity with extremely inert packing materials to answer your difficult method development.