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Mar 24, 2025 | HPLC Columns
 

HPLC Column Selection Guide 

Need help choosing the right HPLC column for your method? This page brings together our most useful selection tools—from regulatory-based guides to comparison charts and downloadable resources. Whether you're developing a new method, optimizing selectivity, or aligning with USP classifications, these links will guide your decision-making.

Explore the resources below to get started.

HPLC Column Selection Guide by USP

Easily find GL Sciences columns grouped by USP classification to meet your compendial or regulatory needs. Learn More

HPLC Columns Specification Comparison

Compare key specifications across GL Sciences’ full HPLC column portfolio—including USP code, particle size, pore size, surface area, carbon load, end-capping, inertness, and pH range.  Learn More

HPLC Column Selection Overview

Explore recommended column types and separation modes based on analyte properties—including polarity, derivatization, and molecular class. Learn More

Reversed Phase Column Selection Guide

Use this guide to select reversed phase columns based on molecular weight, retention behavior, steric selectivity, and π–π interaction strength. Learn More

Normal Phase & HILIC Column Selection Guide

Select normal phase and HILIC columns based on retention needs and selectivity, including options for amino, cyano, silanol, and sugar-polar analytes. Learn More

Selectivity Comparison of Reversed Phase Columns

Compare the selectivity and elution profiles of reversed phase columns under identical conditions using a standard mixture of acidic, basic, and aromatic compounds. Learn More

Normal Phase & HILIC Column Selection Guide

Select normal phase and HILIC columns based on retention needs and selectivity, including options for amino, cyano, silanol, and sugar-polar analytes. Learn More

Selectivity Comparison of Reversed Phase Columns

Compare the selectivity and elution profiles of reversed phase columns under identical conditions using a standard mixture of acidic, basic, and aromatic compounds. Learn More

HPLC Column Equivalent

Find GL Sciences HPLC column equivalents for seamless method transfer or substitution. Learn More

 

Need Help Choosing the Right HPLC Column?

Connect with a GL Sciences HPLC specialist today. Send us your questions, and our experts will help you find the perfect HPLC column for your application.