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About Inertsil ODS-2

Inertsil ODS-2 has a highly inert filler in which a spherical silica gel with a pore diameter of 150 Å was chemically modified and end-capped with an octadecyl group and silanol group, respectively.

 

Figure 1: Comparison of Retentivity

 
Retentivity comparison chromatograms: Inertsil ODS2 vs ODS3 vs ODS-SP (HPLC C18)
 
 
 

Inertsil ODS-2 Applications

 
 

Water-soluble Vitamins (Data No. LA014)

 
Chromatogram — Water-soluble Vitamins
 

Conditions
Column : Inertsil ODS-2 5 µm, 150 × 4.6 mm I.D. (Cat. No. 5020-01101)
Eluent : A) IPCC-05 + 0.1% H3PO4, B) CH3CN/IPCC-05 + 0.1% H3PO4, A/B = 97.5/2.5 – 20 min – 50/50
Flow Rate : 1.0 mL/min
Col. Temp. : 40 °C
Detection : UV 254 nm
Inj. Vol. : —
Analytes :

1. L-Ascorbic acid 2. Nicotinic acid 3. Nicotinamide 4. Pyridoxal 5. Pyridoxamine 6. Pyridoxine 7. Thiamine 8. Folic acid 9. Cyanocobalamin 10. Riboflavin
 
 

Fat-soluble Vitamins (Data No. LA019)

 
Chromatogram — Fat-soluble Vitamins
 

Conditions
Column : Inertsil ODS-2 5 µm, 150 × 0.7 mm I.D. (Cat. No. 5020-13073)
Eluent : CH3CN/CH3OH = 75/25
Flow Rate : 30 µL/min
Col. Temp. : —
Detection : UV 280 nm
Inj. Vol. : —
Analytes :

1. Vitamin A 2. Vitamin D2 3. Vitamin D3 4. Vitamin E 5. Vitamin E acetate 6. Vitamin K1
 
 

Polyaromatic hydrocarbons (Data No. LA305)

 
Chromatogram — Polyaromatic hydrocarbons
 

Conditions
Column : Inertsil ODS-2 5 µm, 250 × 4.6 mm I.D.
Eluent : CH3CN/H2O = 75/25
Flow Rate : 1.0 mL/min
Col. Temp. : 40 °C
Detection : UV 220 nm
Inj. Vol. : —
Analytes :

1. Naphthalene 2. Acenaphthylene 3. Biphenyl (Diphenyl) 4. Fluorene 5. Acenaphthene 6. Phenanthrene 7. Anthracene 8. Benzo(a)anthracene 9. Fluoranthene 10. Pyrene 11. Triphenylene 12. Crycene 13. Benzo(e)pyrene 14. Benzo(a)pyrene 15. Dibenzo(ah)anthracene 16. Benzo(ghi)perylene
 
 

Pesticides (Data No. 262)

 
Chromatogram — Pesticides
 

Conditions
Column : Inertsil ODS-2 5 µm, 150 × 4.6 mm I.D.
Eluent : CH3CN/H2O = 55/45
Flow Rate : 1.0 mL/min
Col. Temp. : 50 °C
Detection : UV 235 nm
Inj. Vol. : —
Analytes :

1. Dimethoate 2. DDVP 3. Methyl parathion 4. Malathion 5. Sumithion 6. Parathion 7. Diazinon 8. EPN

each 0.1%

 
 

View All Application Notes for Inertsil ODS-2

 
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