Guard Column's Selection & Use
VIDEO: How to select an HPLC Guard Column Video (1:49)
Guard columns are installed between the injector and the analytical column of a HPLC system, to protect the analytical column. Guard columns are widely used and are cost effective to prolong the life of HPLC columns.
GL Sciences cartridge design contains a reusable holder and 2 disposable cartridges. Easy to use, and you can change the guards in seconds. Our guard columns are packed with the same material as our analytical columns.
I. Selecting Packing Material
When selecting the packing material for guard columns, we recommend choosing the same material that is contained in your analytical column.
II. Selecting Dimensions & Particle Size
Use the same particle size as your HPLC column. Choose a guard column with an internal diameter similar to the analytical column internal diameter.
Our column coupler is a universal self-adjusting connector used to connect the guard column and the analytical column.
IV. Guard Column Installation
Connectors and tubing are required to properly connect the guard columns to analytical columns. Make sure to select the appropriate connectors depending on the type of end-fittings you use on the analytical column, otherwise, performance may be negatively impacted due to extra dead volume.
V. Type of End Fittings
End-fitting styles differ among various manufacturers and have different seating depths.
Always make sure to select the appropriate end-fittings, otherwise, chromatographic performance will be negatively impacted due to extra dead volume.
VI. Influence of Extra Dead Volume on Chromatographic Performance
Column connectors and tubing are required separately to connect guard columns to analytical columns. Make sure to select the appropriate connectors depending on the type of end-fittings use on the analytical column, otherwise, chromatographic performance will be negatively impacted due to extra dead volume.
GL Sciences Offers a Wide Variety of Guard Columns
Guard Cartridges & Holder
Easy-to-use, convenient, and disposable guard cartridges will protect your analytical columns.
Our cartridge holder is reusable. Cartridges contain the same high-performance packing material that is used in our GL Sciences analytical columns. Shop Guard Columns & Cartridges