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HPLC Columns
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- InertSustain C18 First choice for ultra-high inertness and high durability, delivering superior peak shapes with low back pressure.
- Inertsil ODS-3 Most popular C18, trusted for long-established methods, offering strong retentivity and high loading capacity.
- InertSustain AQ-C18 First choice for high-polarity compounds, delivering strong retention even under 100% aqueous mobile phases.
- Inertsil ODS-3V Validated version of ODS-3, designed for GLP/GMP compliance, ensuring batch-to-batch consistency.
- InertSustainSwift C18 Ultra-inert, fast-separation C18 for high-throughput LC-MS and LC-MS/MS with superior peak symmetry.
- InertSustain AX-C18 Mixed-mode C18 with anion exchange for strong retention of highly polar acidic compounds without ion-pairing.
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- InertSustain C8 Ultra-inert C8 column for rapid analysis of hydrophobic compounds with symmetric peaks across a wide pH range.
- Inertsil C8 Ultra-pure silica C8 column for rapid analysis of hydrophobic compounds, ideal for legacy methods.
- InertSustainSwift C8 Ultra-inert C8 column for low-polarity analytes, peptides, and oligonucleotides with optimized 200Å pore size.
- Inertsil C8-3 Strong retentivity, very inert, and low back pressure, based on the same silica and bonding as Inertsil ODS-3.
- Inertsil WP 300 Wide-pore C8 column for rapid, high-resolution separation of proteins and peptides with sharper peaks.
- Inertsil C4 Low-retentivity C4 column for rapid analysis of highly hydrophobic compounds like fat-soluble vitamins.
- All C8 & C4 Columns visible-xs
- InertSustain Amide First choice for HILIC mode, offering the strongest retention of polar compounds with superior stability in water-rich conditions.
- Inertsil HILIC Diol-bonded HILIC column for excellent peak shape and strong retention of highly polar basic compounds.
- InertSustain NH2 First choice for sugar analysis, offering superior stability, reproducibility, and compatibility with weakly acidic eluents.
- Inertsil NH2 Aminopropyl column for sugar analysis, highly retentive in normal phase with superior stability and reproducibility.
- ProteoSil HILIC Bioseparation-focused HILIC column, excelling in hydrophilic compound, peptide, glycan, and oligonucleotide analysis.
- All HILIC Columns visible-xs
- InertSustain PFP A pentafluorophenyl-modified column designed for enhanced separation of structurally similar compounds through multiple retention mechanisms, including π-π, dipole, and hydrogen bonding interactions.
- InertSustain Phenyl A directly bonded phenyl group gives the InertSustain Phenyl column unique reversed-phase selectivity, ideal for separating polar compounds and structural isomers via π-π and hydrogen bonding interactions.
- InertSustain Phenylhexyl A phenylhexyl-bonded column that offers complementary selectivity to traditional alkyl-chain columns, providing high inertness, reproducibility, and low back pressure.
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- InertSep C18 Silica sorbent with high end-capping, ideal for lipid removal & pesticide residue analysis.
- InertSep C18-ENV C18 sorbent with low end-capping, optimized for water analysis & anionic surfactant removal.
- InertSep HLB Versatile SPE column for extracting non-polar to highly polar compounds, with a wide pH range (1-14).
- InertSep C8 Silica sorbent with octyl groups, offering weaker retention than C18 for highly retained compounds.
- InertSep SCX Silica-based SPE column with strong cation exchange and non-polar interactions for enhanced retention.
- InertSep SAX Monofunctional C18 sorbent with medium end-capping, allowing secondary interactions for versatile use.
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- MonoSpin C18 High-permeability spin column for peptide desalting, drug extraction, and fast biological sample preparation.
- MonoSpin ProA Fast Protein A spin column for high-purity IgG antibody purification with >90% recovery.
- MonoSpin C18-CX Mixed-mode spin column with C18 and cation exchange for enhanced basic drug recovery.
- MonoSpin Phospholipid TiO₂/ZrO₂-coated spin column for efficient phospholipid removal and matrix effect reduction.
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InertSep QuEChERS Kit High-purity extraction and cleanup kit for pesticide residue analysis in food and environmental samples.
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GC Columns
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- InertCap 5MS/NP General purpose, Halogenated compounds, Phenols, Pesticides, FAME
- InertCap 5 General purpose, Halogenated compounds, Phenols, Pesticides, FAME
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Instruments
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LD249 Gas Leak Detector Compact, easy-to-use leak detector with thermal conductivity sensor for fast, accurate gas detection. Replaces the LD239. -
FM4: Air Sampler for PFAS Low-volume air sampler for PFAS enabling comprehensive analysis of both gas and particle phases in a single sampling using stage-optimized materials. -
Intelligent Pump UI-22 Series Low-pulsation linear motor pump with precise flow control and bubble-free operation. -
OralChroma Halitosis Measuring Device High-precision breath analyzer using gas chromatography to quantify VSCs in 4 minutes — fast, reliable, and maintenance-free.
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LD249 Gas Leak Detector Latest model replacing LD239, with thermal conductivity detection for helium, hydrogen, and other gases.
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GF1010 Digital Gas Flowmeter Portable, volumetric meter for accurate GC gas flow measurement without gas selection.
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Intelligent Pump UI-22 Series Low-pulsation linear motor pump with precise flow control and bubble-free operation.
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Dual Pump KP-21 Series High-precision, medium to high-pressure pump with RS-232C control and customizable seals. -
Auto BPR BP-11 Auto Back Pressure Regulator for the UI-22 Series, maintaining stable pressure for consistent flow. -
PCS Pump SP-22 Series Single plunger pump with a pressure sensor, pulse control, and real-time pressure display for precise monitoring. -
Dual Pump KP-22 Series High-precision, low-pulsation pump with pulse control, real-time pressure display, and RS-232C control. -
PCS Pump SP-21 Series Cost-effective single plunger pump with pulse control, ideal for basic solvent dispensing. - All HPLC Pumps visible-xs
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Gastorr AG Series Reliable, CPU-controlled degassing system for low-flow applications with independent chamber design. -
Gastorr BG Series High-performance degassing system with real-time vacuum display and independent chamber design. -
Gastorr FG-42 Solvent degassing system optimized for fluorinated solvents with real-time vacuum display. -
Gastorr PG Series High-flow degassing system for fractionating applications with real-time vacuum display. - All Degassing Units visible-xs
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- PFAS Solutions Products and workflows for EPA and ISO-compliant PFAS testing in water, air, and soil.
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- HPLC Columns
- Sample Preparation
- GC Columns
-
Instruments
-
LD249 Gas Leak Detector Compact, easy-to-use leak detector with thermal conductivity sensor for fast, accurate gas detection. Replaces the LD239.
-
FM4: Air Sampler for PFAS Low-volume air sampler for PFAS enabling comprehensive analysis of both gas and particle phases in a single sampling using stage-optimized materials.
-
Intelligent Pump UI-22 Series Low-pulsation linear motor pump with precise flow control and bubble-free operation.
-
OralChroma Halitosis Measuring Device High-precision breath analyzer using gas chromatography to quantify VSCs in 4 minutes — fast, reliable, and maintenance-free.
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Solutions for Testing & Analysis of PFAS Compounds in the Environment
Per- and polyfluoroalkyl substances (PFAS) are resistant to degradation and are highly persistent compounds in the environment. Today, PFAS contamination is a major concern in drinking water, soil, air, and animal tissue leading to stringent new regulations for testing and analysis of these compounds.
GL Sciences strives to support initiatives toward managing the contamination of PFAS compounds in the environment. We provide a diverse range of solutions for various steps in the workflow for PFAS analysis, designed to meet rigorous modern regulatory requirements.
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PFAS Testing & Analysis Products
SPE Columns for PFAS Testing & Sample Preparation
Efficiently purify PFAS samples using InertSep SPE columns. InertSep columns provide excellent sample recovery and are compatible with major methods for PFAS analysis. Select from multiple column formats and sizes to easily scale your process.
SPE Columns Product Line
| InertSep MA-2 | InertSep MA-2 is a methacrylate polymer-based sorbent modified with, which includes a weak anion exchange function group. Because polymer base gel occurs little secondary interaction, SPE by genuine anion exchange interaction can be achieved with this sorbent. |
| InertSep WAX | InertSep WAX FF is a sorbent based on styrene divinylbenzene polymer modified by a weak anion-exchange group. Its reversed-phase, anion-exchange action and exceptional retention of acidic compounds make it suitable for purification of samples containing basic and neutral impurities. By controlling the pH, it can be used to recover acidic compounds under basic conditions. |
| InertSep PLS-3 | InertSep PLS-3 is a copolymer based sorbent comprised of nitrogen-containing methacrylate and SDB, it exhibits a wide enough range of retention capability from highly polar to hydrophobic compounds. |
| InertSep PLS-2 | InertSep PLS-2 is a SDB (Styrene divinylbenzene) polymer-based reversed phase mode sorbent. Compared to a silica based C18 sorbent, InertSep PLS-2 has a quite higher sorbent retentive capacity and better stability in pH range. |
| InertSep HLB | InertSep HLB is a solid-phase extraction column that can effectively extract compounds with a wide range of physical properties from non-polar to high polar. |
| InertSep GC | InertSep GC cartridges are packed with graphite carbon in planar structure. They are generally used for removal of pigments from crop homogenates. In conjunction with other various normal phase sorbents and ion exchange sorbents, these cartridges are able to be used for a wide variety of applications as a cleanup sorbent. |
Select suitable phases for the appropriate separation mode of your method.
Easily scale your workflow for the necessary size and speed with different column sizes
Elution using InertSep provides excellent recovery of samples even with small amounts of solvent.
SPE cartridge: InertSep MA-2 250 mg
Solvent: 0.1% ammonium methanol
Elution volume: 1 mL x 10
FM4: Air Sampler for PFAS
Simplify your sampling process for PFAS compounds in air with GL Sciences' FM4 Air Sampler for PFAS. The FM4 can comprehensively sample existing PFAS compounds in atmospheric particulate and the gaseous phase in one sampling in a compact and portable package. Streamline your sampling process for PFAS in air today.
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The FM4 Air Sampler for PFAS is a low-volume air sampler that can comprehensively sample various types of PFAS compounds in air. The FM4 uses multiple different adsorbents to capture existing PFAS compounds in both atmospheric particulate and gaseous phase in one sampling. The FM4 can sample PFAS compounds such as FTOH, PFSA, FOSA, and many other PFAS compounds of high interest with a high recovery rate.
- Comprehensively samples various types of PFAS compounds in air
- Simultaneous sampling using several different adsorbent layers
- Captures high-interest PFAS compounds such as FTOH and PFSA
- Excellent recovery rate of target compounds
- Compact and portable package
Excellent Recovery Rate for Volatile PFAS such as FTOHs and FOSEs. The FM4 boasts high recovery rates of gaseous and particulate PFAS.
Compact and Portable design with easy set-up
LC Columns for PFAS Analysis
GL Sciences HPLC columns are method-compatible and provide excellent separation of PFAS compounds. GL Sciences HPLC columns for PFAS analysis include a selection of high-performance C18 analytical columns and a delay column for reducing noise from background PFAS.
LC Columns Product Line
| InertSustain AQ-C18 | InertSustain AQ-C18 columns are designed to achieve strong retention for highly polar compounds, which is the most challenging goals in developing reversed phase methods. The optimization of bonding of the C18 groups at equal distance to the silica gel enable InertSustain AQ-C18 columns to offer significant retention for highly polar compounds even under water rich mobile phases. |
| InertSustain C18 | Generally, silica based columns are mechanically stable and provide high efficiencies, however, they cannot be used under alkaline conditions and their residual silanol groups tend to adsorb organic bases. InertSustain C18 employs a radically new type of silica, in which the surface of the silica is uniquely modified, enabling precise control of the silica properties. InertSustain C18 inherits the advantages of all the current Inertsil HPLC columns (e.g., extremely low operating back pressure, superior inertness to typically any analytes, high efficiency and compatibility with a wide range of solvents), but now can be used for wide pH analysis with consistent performance from column to column and lot to lot. |
| Delay Column for PFAS | Delay Column for PFAS is packed with high-purity activated carbon, which is able to retain contaminants originating from the mobile phase or the LC system. Elution of retained contaminants is delayed allowing for the effects of the contaminants on the analysis to be reduced. This column is quite useful for PFAS (Per- and Polyfluoroalkyl Substances) analysis on LC/MS. |
GL Sciences C18 phases such as InertSustain C18 and InertSustain AQ-C18 deliver excellent separation performance and resolution of PFAS compounds.
The Delay Column for PFAS provides better separation by isolating background PFAS from the LC system and mobile phase and is installed between the pump and autosampler/injector.
The resolution for the sample PFAS is substantially improved with excellent isolation from the Delay Column for PFAS
InertSustain HPLC columns are designed to provide exceptional stability, reproducibility, and performance. Employing a radically new type of silica, InertSustain columns are highly end-capped for superior inertness and compatibility with a wide range of solvents.
Other Recommended PFAS Testing & Analysis Products
| SKU | Product |
| 1030-14000 | High Purity PP Vial (Screw) 0.3ML HIGH PURITY PP SCREW VIAL 100/PK CLEAR0.3ML HIGH PURITY PP |
| 1030-72000 | High Purity PP Vial with Caps(Snap) 9-425 PP green screw cap alumi/silicone 100pcs(cap color green) |
| 1030-72001 | High Purity PP Vial with Caps(Snap) 9-425 PP yellow screw cap alumi/silicone 100pcs(cap color yellow) |
| 1030-14004 | High Purity PP Vial (Screw) 0.3 mL 1000 pcs. |
| 1010-68142 | InertCap Pure-WAX GC Column, 30 m x 0.25 mm, 0.25 µm df |
EPA & ISO Methods for PFAS Detection & Testing
GL Sciences supports major PFAS detection & testing methods from regulatory bodies in the U.S. and internationally with various solutions from sampling of PFAS to sample preparation and analysis. Refer to references below to see how you can use GL Sciences solutions for your workflow.
EPA Method 537.1
EPA Method 537.1 is commonly used to test PFAS in drinking water compliance. EPA Method 537.1 analyzes 14 compounds from the original EPA Method 537 and 4 replacement compounds.
GL Sciences provides InertSep PLS-2 SPE column for sample preparation and InertSustain AQ-C18 with a Delay Column for analysis using LC-MS/MS.
EPA Method 533
EPA Method 533 analyzes drinking water and is complementary to EPA Method 537.1. EPA Method 533 tests 25 compounds.
GL Sciences provides InertSep MA-2 and InertSep WAX SPE columns for sample preparation. The same InertSustain AQ-C18 with a Delay Column can be used for analysis using LC-MS/MS.
List of PFAS testing methods
For other methods and associated products, please refer to the following list:
| Method name | Year published | For | Number of compounds analyzed | Instrumentation | Associated products | |
| ISO 25101: 2009 | Water quality — Determination of perfluorooctanesulfonate (PFOS) and perfluorooctanoate (PFOA) — Method for unfiltered samples using solid phase extraction and liquid chromatography/mass spectrometry | 2009 | Drinking water, Ground water, Surface water | 2 | LC-MS/MS | InertSep PLS-2, Delay Column for PFAS, InertSustain AQ-C18 |
| ISO 21675: 2019 | Water quality — Determination of perfluoroalkyl and polyfluoroalkyl substances (PFAS) in water — Method using solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) | 2019 | Drinking water, Natural water (fresh water and sea water), Waste water containing <2g/L solid particulate material (SPM) | 30 | LC-MS/MS | InertSep WAX, Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Method 533 | Determination of Per- and Polyfluoroalkyl Substances in Drinking Water by Isotope Dilution Anion Exchange Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry | 2019 | Drinking water | 25 | LC-MS/MS | InertSep WAX, InertSep MA-2, Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Method 537.1 | Method 537.1: Determination of Selected Per- and Polyfluorinated Alkyl Substances in Drinking Water by Solid Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) | 2018 (2020) | Drinking water | 14 | LC-MS/MS | InertSep PLS-2, Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Draft Method 8327 | Per-and Polyfluoroalkyl Substances (PFAS) by Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS) | 2021 | Surface water, Ground water, Waste water matrices | 24 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Draft Method 8328 | - | - | Surface water, Ground water, Waste water solids | 25 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Draft Method 8329 | - | - | Soil | 24 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
| EPA Method 1633 (Third Draft) | Analysis of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous, Solid, Biosolids, and Tissue Samples by LC-MS/MS | 2021 (2024) | Aqueous, Solid, Biosolids, and Tissue samples | 40 | LC-MS/MS | InertSep WAX, InertSep MA-2, InertSep GC, Delay Column for PFAS, InertSustain AQ-C18 |
| ASTM D7979-20 | Standard Test Method for Determination of Per- and Polyfluoroalkyl Substances in Water, Sludge, Influent, Effluent, and Wastewater by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) | 2020 | Water, Sludge, Influent, Effluent, and Wastewater | 21 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
| ASTM D7968-17a | Standard Test Method for Determination of Polyfluorinated Compounds in Soil by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) | 2014 (2023) | Environmental solids: Soil, Sediment, Sludge | 30 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
| DIN 38407-42:2011 | German standard methods for the examination of water, waste water and sludge | 2011 | Water, Waste water, and sludge | 10 | LC-MS/MS | InertSep WAX, InertSep MA-2, Delay Column for PFAS, InertSustain AQ-C18 |
| ASTM D8421-22 | Standard Test Method for Determination of Per- and Polyfluoroalkyl Substances (PFAS) in Aqueous Matrices by Co-solvation followed by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS) | 2022 | Aqueous matrices | 44 | LC-MS/MS | Delay Column for PFAS, InertSustain AQ-C18 |
PFAS Detection & Testing Applications
Find reference analyses from our list of available applications for PFAS compounds.
Get Help With Your PFAS Analysis
Connect with a GL Sciences PFAS specialist today. Send us your questions, and our team will provide the expertise you need to help you with your PFAS analysis.