Technical Notes

Adjustable range of chromatography conditions has been specified in [2.00 General theory of chromatography] which has been stipulated based on the contents harmonized and agreed upon by Japanese/U.S./European Trilateral Pharmacopoeia at the first supplemental public comment of Japanese Pharmacopoeia, 18th Edition, which was disclosed in September 2021. Based on this, the acceptable changeable range of conditions is widened, enabling easier requirement study. In this report, analysis was implemented by changing the column size within the range specified in [2.00 General theory of chromatography] for JP18 Purity test (5) of saccharin sodium hydrate. The change of analytical conditions is for the content to be applied from the first supplemental revision of JP18 and is not for the content ...

<style type="text/css"><!--td {border: 1px solid #ccc;}br {mso-data-placement:same-cell;}--></style> <span data-sheets-value="{&quot;1&quot;:2,&quot;2&quot;:&quot;Presented here is an analysis of ethanol in food using FID mounted gas chromatograph dedicated to packed column.For the analysis of sample containing non-volatile or refractory components, selecting an insert injection method and by keeping the residual contaminants within an injection port liner, the impact on analytical column can be reduced. In the analysis of soy sauce or soybean paste used in this example, the sample can be quantitated only by dilution.&quot;}" data-sheets-userformat="{&quot;2&quot;:513,&quot;3&quot;:{&quot;1&quot;:0},&quot;12&quot;:0}">Presented here is an analysis of ethanol in food using FID mounted gas ...

<style type="text/css"><!--td {border: 1px solid #ccc;}br {mso-data-placement:same-cell;}--></style> <span data-sheets-value="{&quot;1&quot;:2,&quot;2&quot;:&quot;Adjustable range of chromatography conditions has been specified in [2.00 General theory of chromatography] which has been stipulated based on the contents harmonized and agreed upon by Japanese/U.S./European Trilateral Pharmacopoeia at the first supplemental public comment of the Japanese Pharmacopoeia, 18th Edition, which was disclosed in September 2021. Based on this, the acceptable changeable range of conditions is widened, enabling easier requirement study.\r\nIn this report, an analysis was implemented by changing the column size within the range specified in [2.00 General theory of chromatography] for JP18 Purity Test ...

Kasugamycin is widely used as an agricultural fungicide. In this study, kasugamycin added to "brown rice", "orange", and "peanut" extracts was extracted and purified using a mixed-mode polymer solid-phase (InertSep MCX FF) with reference to a method complied with the notification test method of the Ministry of Health, Labor and Welfare. Analysed by LC / MS / MS.

Malachite Green (MG) is a blue-green basic pigment that is used as a remedy against aquatic fungal diseases, it is also used as a bactericidal agent for farmed fish. Current research suggests that it may be carcinogenic, therefore use in foods has been banned in the United States, EU, etc. The distribution and sale of foods in which malachite green is detected is banned in Japan. However, since on rare occasions violations of this ban are confirmed in imported foods, etc., tests are widely conducted by inspection organizations. In this study, malachite green and its biotransform Leucomalachite Green (LMG) were extracted from grilled eel extract and purified using a mixed-mode polymer solid phase (InertSep MCX FF, LMG) and then analyzed by LC / MS / MS with a test method complied with ...

When the pH of mobile phase is changed in the analysis of reverse phase mode on HPLC, the dissociation / non-dissociation state changes depending on the compound, so the retention time changes. Normally, the retention time is longer in non-dissociated state and shorter in dissociated state. This time, using Inertsil WP300 C18, we analyzed the basic compound saccharin and the acidic compound phthalic anhydride standard, and found that the elution order changed under acidic and neutral conditions. We also report that it was estimated that phthalic anhydride in the sample was partially changed to phthalic acid.

Glucosamine is an amino sugar in which one of the hydroxy groups of glucose is replaced with an amino group, and is often used in health foods. At the 10th Edition Food Additive Official Formulation Study Group (3rd), a draft ingredient standard using liquid chromatography as a quantification method for glucosamine was decided. This time, we would like to introduce the analysis of glucosamine that complies with the draft component standard using the Hitachi HPLC system Chromaster.

Malic acid is one of the organic acids and is widely present in foods and living organisms. Analysis of the malic acid standard reagent confirmed the phenomenon that two peaks appeared depending on the detection method. This time, we analyzed malic acid using a diode array detector, and it was estimated that the impurity contained in malic acid was fumaric acid.

ß-Cryptoxanthin is a carotenoid pigment found in the edible part of the fruit Citrus unshiu. In addition to its function as provitamin A, which is mainly metabolized to the essential nutrient vitamin A, it is also known to have a excellent antioxidant properties derived from the highly conjugated double bonds in the molecular framework. ß-Cryptoxanthin is considered to be effective in preventing lifestyle-related diseases and in reducing the incidence of osteoporosis. Many foods, supplements, soft drinks, etc. are commercially available that take advantage of these beneficial effects. In this application note, separation and analysis was made with reference to the food sanitation inspection guidelines, and the JAS amendment.

Assuming a fire site where fuels such as gasoline, kerosene, and light oil were used, we used HandyTD TD265 to perform a concentration analysis of the components that volatilize from the recovered material after combustion. In the recovered material, components derived from the fuel used for combustion were detected.

InertCap ProGuard is a capillary column with an integrated guard column, which protects the column from impurities that may be present in the sample.? With InertCap ProGuard, the life of the capillary column can be extended, even when analyzing samples containing large quantities of contamination.

The 17th revision of Japanese Pharmacopoeia stipulates a method using gas chromatography as a method for measuring the alcohol content in products. This time, we will report the results of pretreatment and analysis with reference to "Alcohol number measurement method" test for Pharmacopoeia.

In this note, it is shown the analysis of UV absorbers in cosmetic products.<br />UV absorbers contained in cosmetic products are compounds that prevent the skin from being penetrated and burned by UV rays by absorbing the energy of UV rays and converting it into thermal energy.<br />The compounds are analyzed below. They are used in several products commonly available in the market with sunscreen formulation such as cosmetics, hair care products, and perfumes.<br />In this note, 8 compounds of UV absorbers were analyzed by gradient elution. The results show an excellent separation of the compounds.