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SPE Columns / Cartridges

Columns with pre-packed sorbents for sample pre-treatment by solid phase extraction. GL Sciences InertSep SPE column sorbents come in two types of sorbents: polymer-based sorbents which have the widest pH compatibility and silica-based sorbents which are more cost-effective and have higher physical strength. Columns are available in various sizes and formats including cartridges, luer-type devices and 96-well plates for compatibility with every SPE method (gravity, vacuum, positive-pressure, to centrifugal systems). Read more

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InertSep GC cartridges are packed with graphite carbon in planar structure. They are generally used for removal of pigments from crop homogenates. In conjunction with other various normal phase sorbents and ion exchange sorbents, these cartridges are able to be used for a wide variety of applications as a cleanup sorbent. Specifications B...
From $240.00
InertSep GC-e cartridges are packed with graphite carbon which has similar physical properties to InertSep GC. InerSep GC-e provides the same extraction performance as InertSep GC at low cost. Specifications Base Gel: Graphite Carbon Average Particle Size: 100/200 mesh Surface Area: 90 m2/g Pore Volume: 1 mL/g Pore Size: 50 nm
From $150.00
InertSep for AQUA is a SPE cartridge with low background contamination. Without conditioning, it is possible to conduct sample preparation easily and decrease solvent consumption by 50 % in procedure.
From $530.00
These two layer cartridges are packed with graphite carbon for removing pigments and NH2 sorbent for sample cleanup of organic extracts. This consolidation of two layers achieves high sample cleanup performance which cannot be obtained only on one layer.
From $340.00
These two layer cartridges are packed with graphite carbon for removing pigments and PSA sorbent for sample cleanup of organic extracts. This consolidation of two layers achieves high sample cleanup performance which cannot be obtained only on one layer.
From $290.00
InertSep PA is a special SPE modified with polyamide functional groups. Primary interactions are anion exchange and polar exchange. Secondary interactions are non-polar exchange.
From $300.00
InertSep Slim-J DRY cartridge is packed with anhydrous Na2SO4 for dehydration.
From $230.00
InertSep K-solute is packed with diatomaceous earth and ideal even for the sample to form an emulsion during liquid-liquid extraction procedure. Dedicated rack for InertSep makes the operation simple and efficient.
From $160.00
A large number of mycotoxins are contained in natural food products. Among these, Aspergillus flavus is known to produce aflatoxins that are carcinogenic to liver cells. Mycotoxins in food safety have received a great deal of attention in recent years.
From $150.00
InertSep mini AERO series are active samplers for the analysis of Aldehydes and Ketones in outdoor gas, car cabin and exhaust gas in compliance with: Offensive Odor Control Law, Clean Air Act, and EPA. There are four types: AERO DNPH, AERO DNPH-HR, AERO Ozone Scrubber, and AERO SC, InertSep mini AERO series.
From $220.00
InertSep Phospholipid is developed for removal of phospholipids selectively and is able to minimize ion suppression of LC/MS/MS analysis. It can remove more than 90 % of phospholipids.
From $340.00
Kits that combine various products in the InertSep series.
From $230.00
These two-layer cartridges are packed with GC-e graphite carbon for removing pigments and NH2 sorbent for sample cleanup of organic extracts. Due to the cost reduction of GC-e, these two-layer cartridges achieve high sample cleanup performance at low cost.
From $210.00
These two-layer cartridges are packed with GC-e graphite carbon for removing pigments and PSA sorbent for sample cleanup of organic extracts. Due to the cost reduction of GC-e, these two-layer cartridges achieve high sample cleanup performance at low cost.
From $200.00
SKU# 5010-30250
The EZ Cartridge RP-1 is a new type of solid-phase extraction cartridge comprising a solid-phase membrane in a Luer-compatible housing. Use of Empore membrane technology and a large-diameter design greatly increases the volume and flow rate of sample water passing through the cartridge, significantly reducing sample preparation time. The ...
$650.00
SKU# 5010-68034
InertSep GC/PSA/SI is more efficient for removing a wide variety of polar matrix compounds than GC/PSA.
$340.00
SKU# 5010-68044
InertSep GC/SAX/PSA are able to be used for cleanup of processed food.
$430.00
SKU# 5010-68054
InertSep GC/SAX/PSA/SI is a four layer SPE cartridge. GC has ability for the isolation removal of pigments and SAX, PSA and SI offer superior cleanup when conducting multi-residue pesticide analysis.
$450.00
SKU# 5010-68114
InertSep SAX/PSA/SI is a three layer SPE cartridge for cleanup. It is available for sample cleanup to make analysis of residual pesticides.
$360.00
SKU# 5010-25500
InertSep Slim-J AC cartridges are packed with active carbon particles uniformly size classified for liquid permeability. As ultra-pure active carbon is employed, it is clear of worry about contamination. Good retentivity of this sorbent even for highly polar compounds ensure high recovery and reproducibility. Luer device format supports a...
$570.00
SKU# 5010-65780
InertSep Slim-J AERO SDB400 is a cartridge for collecting semi-volatile organic compounds (SVOC) in the atmosphere. In addition to collecting pesticides and flame retardants in the atmosphere, it is also suitable for collecting naphthalene added to the target components for work environment measurement.
$250.00
SKU# 5010-68133
InertSep C18/DRY is a two-layer SPE cartridge and designed for sample preparation for residual pesticide analysis. C18 is to remove lipids and DRY is for dehydration.
$220.00
SKU# 5010-68344
Due to the cost reduction of GC-e, this three-layer cartridge achieves high sample cleanup performance at low cost.
$260.00
SKU# 5010-61000
$210.00
SKU# 5010-61001
$250.00
SKU# 5010-61002
$180.00
SKU# 5010-61003
$260.00
SKU# 5010-61004
$180.00
SKU# 5010-61005
$190.00
SKU# 5010-61006
$220.00
SKU# 5010-61007
$450.00
SKU# 5010-61008
$610.00
SKU# 5010-61009
$870.00
SKU# 5010-61010
$620.00
SKU# 5010-61011
$1,060.00
SKU# 5010-61012
$1,480.00
SKU# 5010-61013
$200.00
SKU# 5010-61014
$180.00
SKU# 5010-61015
$170.00
SKU# 5010-61025
$300.00
SKU# 5010-61026
$360.00

Available Formats for SPE Columns

InertSep SPE Columns are available in the following formats:

 

Cartridge

Cartridge format
  • Cartridge volume: 1, 3, 6, 12, 20, 60, 150 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Processing of small liquid samples up to bulk

96-well plate

 
96-well plate format
  • Cartridge volume: 1, 2 mL 
  • Cartridge material: PP housing and PE frit
  • Purpose: Rapid processing for multiple samples
 
 

Luer Device type

 
Slim and Slim-J Luer device format

Slim & Slim-J type​

  • Dimensions: Slim: 8.8 mm O.D. length 32 mm, 21mm; Slim-J: 8.8 mm O.D. length 51 mm, 31 mm
  • Cartridge material: PP housing and PE frit 
  • Purpose: Tandem processing and automatic SPE system 
InertSep Luer mini format

Mini type

  • Dimensions: 12.7 mm O.D. length 32 mm, 21 mm 
  • Cartridge material: PP housing and PE frit 
  • Purpose: manual processing, tandem processing and automatic SPE system
 

Concept of Solid Phase Extraction (SPE)

Main approaches of SPE

The principle of SPE is divided into the following two methods:

1. Retention of Target Analyte

 
Retention of Target Analyte in SPE

2. Retention of Sample Matrix

 
Retention of Sample Matrix in SPE
 

General Steps of SPE

General Steps of SPE
 

General Methods for Sample Processing

Solid phase extraction methods include: gravity-based, positive pressure, vacuum, and centrifugal separation systems. 

General Methods for Sample Processing in SPE
 

Available Sorbents for SPE Columns

InertSep SPE cartridges come in two major types of base gels - Polymer-based sorbents and Silica-based sorbents. Specialty phases are also available. 

 

Polymer-based Sorbents

The advantages of polymer-based sorbent are their availability in a wide pH range and the absence of secondary interaction which can occur with silica-based sorbents.

 

Silica-based Sorbents

Silica-based sorbent materials are more cost-effective and have higher physical strength compared with polymer-based sorbent materials. Silica offers a wide variety of separation mechanisms using a combination of primary functional group interaction with secondary interactions due to the nature of silica. 

 

Specialty Phases

 

Retentive Capacity of a Sorbent Compared to Sorbent Mass

Retentive Capacity of SPE Sorbent compared to sorbent mass

Bed volume is the quantity of the solvent necessary to replace the air trapped in the solid phase. Void volume is equivalent to the bed volume.

 
 

How to Select a Sorbent

 

One of the most important elements to achieve successful solid phase extraction is selection of a sorbent which should be suitable for both the sample matrix and the target analyte. The sorbent should be carefully selected, taking into account the chemical properties of the target analyte, such as its solvent solubility and the physical nature of the sample matrix. In addition, it is important to develop conditions that are optimal for retaining the target analyte, while removing the sample matrix, then selecting an elution solvent for maximum recovery of the target analyte.

How to select an SPE sorbent

Recommendation for Selecting an Ion Exchange Sorbent

 
Differences in selectivity between SPE phases
 

In order to effectively utilize the solid phase extraction method, it is important to choose sorbent mass and size appropriate to loading amount of the matrix and the target analyte. Generally, the total retentive capacity of cartridge type (including matrix) is said to up to 5 % of the sorbent mass.

Recommendation for Selecting an Ion Exchange Sorbent

 

InertSep Quality Control

InertSep SPE columns are manufactured under strict quality control at our Fukushima factory which has obtained International Organization for Standardization ISO-9001 certification.

 

SPE Columns / Cartridges Resources:​

  • Download our entire 2022 Consumables Catalog Download
  • Search Application Notes For SPE Columns / Cartridges — Search Applications Database
  • SPE Column / Cartridge selection guide — Download the guide to select the right sorbent and column / cartridge Download
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