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Reversed Phase HPLC Columns

Reverse Phase Columns

Experience a wide selection of stationary phases for reverse phase HPLC with GL Sciences reverse phase columns. Choose from C18, C8, C4, phenyl, and cyano functional groups to achieve optimal analyte selectivity. Available in multiple particle sizes and column formats, these columns feature high-purity silica and advanced end-capping technology.

Reverse Phase Columns
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Reverse Phase Column List

 
Column Features USP Code Particle Size (µm) Pore Size (nm) Surface Area (m2/g) Carbon Loading End-Capping Inertness Recommended pH range
InertSustain C18 First choice with ultra high inertness and high durability L1 2, 3, 5, 10 10 350 14 Yes ★★★★★ 1 - 10
InertSustain AQ-C18 First choice for high polar compounds L1, L96 1.9, 3, 5 10 350 13 Yes ★★★★★ 1 - 10
InertSustainSwift C18 First analysis with ultra high inertness and high durability L1 1.9, 3, 5 20 200 9 Yes ★★★★★ 1 - 10
InertSustain AX-C18 Analysis of anionic highly polar compounds L1, L78 3, 5 20 200 8 Yes ★★★★ 1 - 9
InertCore Plus C18 Ideal for analyses requiring a high number of theoretical stages L1 2.6 9 200 15 Yes ★★★★ 1 - 10
Inertsil ODS-HL Ultra high retentivity, High-density bonding of C18 phase L1 3, 5 10 450 23 Yes ★★★★★ 2 - 7.5
Inertsil ODS-4 Ultra high inertness, High plate count, Medium retentivity L1 2, 3, 5 10 450 11 Yes ★★★★★ 2 - 7.5
Inertsil ODS-4V Inertsil ODS-4 Validated column L1 3, 5 10 450 11 Yes ★★★★★ 2 - 7.5
Inertsil ODS-3 Strong retentivity, Lower column backpressure, Very inert L1 2, 3, 4, 5, 10 10 450 15 Yes ★★★★ 2 - 7.5
Inertsil ODS-3V Inertsil ODS-3 Validated column L1 3, 5 10 450 15 Yes ★★★★ 2 - 7.5
Inertsil ODS-SP Weak retentivity, for hydrophobic compounds L1 3, 5 10 450 8.5 Yes ★★★★ 2 - 7.5
Inertsil ODS-P High steric selectivity L1 3, 5 10 450 29 None ★★★ 2 - 7.5
Inertsil ODS-EP A polar functional group embedded L1 5 10 450 9 None ★★★★ 2 - 7.5
Inertsil WP300 C18 Analysis of high molecules L1 5 30 150 9 Yes ★★★★ 2 - 7.5
Inertsil ODS-2 Ultra pure silica gel is used L1 5 15 320 18.5 Yes ★★★★ 2 - 7.5
Inertsil ODS Inertness 1st generation L1 5,10 10 350 14 Yes ★★ 2 - 7.5
InertSustain C8 First choice with ultra high inertness and high durability L7 2, 3, 5 10 350 8 Yes ★★★★★ 1 - 10
InertSustainSwift C8 High inertness and high durability C8 column L7 1.9, 3, 5 20 200 6 Yes ★★★★★ 1 - 10
Inertsil C8-4 Ultra high inertness, High plate count, Low retentivity L7 2, 3, 5 10 450 5 Yes ★★★★★ 2 - 7.5
Inertsil C8-3 Strong retentivity, Lower column backpressure, Very inert L7 2, 3, 5, 10 10 450 9 Yes ★★★★ 2 - 7.5
Inertsil C8 Ultra pure silica gel is used L7 5 15 320 10.5 Yes ★★ 2 - 7.5
Inertsil C4 Low retentivity L26 5 15 320 7.5 Yes ★★★★ 2 - 7.5
Inertsil WP300 C8 Suitable for high molecules L7 5 30 150 4 Yes ★★★★ 2 - 7.5
Inertsil WP300 C4   L26 5 30 150 3   ★★★★ 2 - 7.5
InertSustain PFP Extremely Strong retention of highly polar basic compounds. L43 3, 5 10 350 10 Yes ★★★★★ 2 - 7.5
InertSustain Phenylhexyl Strong π-π interactions and hydrophobic interactions L11 3, 5 10 350 9 Yes ★★★★★ 1 - 10
InertSustain Pheny Extremely strong π-π interactions L11 2, 3, 5 10 350 10   ★★★★ 2 - 7.5
Inertsil Ph-3 Strong π-π interactions L11 2, 3, 5 10 450 9.5   ★★★ 2 - 7.5
Inertsil Ph High inertness, Weak π-π interactions L11 5 15 320 10 Yes ★★★ 2 - 7.5
InertSustain Cyano Ultra inertness and can be used in reversed phase mode L10 3, 5 10 350 8 Yes ★★★★ 2 - 7.5
 

Comparison of Phases

 

The figure below shows the strength of the stereoselectivity and the pi-interaction with respect to the holding strength of the reverse phase column. The retention strength, stereoselection system, and strength of pi-interaction are based on the retention coefficient of amylbenzene, retention ratio of o-terphenyl and triphenylene, and retention ratio of amylbenzene and triphenylene, respectively. These are plotted below. In addition, the polar group-encapsulating (embed) type column provides a large effect on other interactions; thus, it was plotted in the type in order to distinguish it from the other columns.

 
Comparison of stereoselectivity between GL Sciences reversed phase columns
 
Strength of pi electron interaction between GL Sciences reversed phase columns
 

Selectivity Comparison of Reversed Phase Columns with Sample Analytes

 

Selectivity of reversed-phase columns are compared under the same conditions, packing material size and column size. Chemical structural formulas used for the comparison are shown at right. Separation differences between basic compounds, acidic compounds, alkyl benzenes and polyaromatic compounds shows the differences of column selectivity. The more silanol groups on the packing material lead the later elution time of caffeine compared with that of phenol. The higher hydrophobicity of the column shows the later elution time of n-Amylbenzene compared with that of n-Butylbenzene. The higher steric selectivity of the column shows the later elution time of Triphenylene compared with that of o-Terphenyl.

 

Conditions

 
Column Reversed Phase Column (5 µm, 250 × 4.6 mm I.D.)*
Eluent A) CH3OH
B) H2O
A/B = 80/20, v/v , (Inertsil Diol, CN-3)
A/B = 70/30, v/v ,
Flow Rate 1.0 mL/min*
Col. Temp. 40 °C
Detection UV 254 nm
Injection Vol. 5 µL*

* For Mono Clad C18-HS (3.0 mm I.D.), 0.4 mL/min of flow rate and 2 µL of injection volume are adopted according to the column internal diameter.

Analyte structures and labels
 
 
Set of Spectra for GL Sciences Reverse phase columns
 
Set of Spectra for GL Sciences Reverse phase columns 2
 
 

Reversed Phase Column Selection

 
For Molecular Weight <5,000
 
Reversed phase column selection for molecular weight less than 5000
 
For Molecular Weight >5,000
 
Reversed phase column selection for molecular weight more than 5000
 
For Highly Polar Compounds
 
Reversed phase column selection for highly polar compounds
 
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