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Reverse Phase Columns

Experience a wide selection of stationary phases for reverse phase HPLC with GL Sciences reverse phase columns. Choose from C18, C8, C4, phenyl, and cyano functional groups to achieve optimal analyte selectivity. Available in multiple particle sizes and column formats, these columns feature high-purity silica and advanced end-capping technology.

Reverse Phase Columns
 
 

Reverse Phase Column List

 
ColumnFeaturesUSP CodeParticle Size (µm)Pore Size (nm)Surface Area (m2/g)Carbon LoadingEnd-CappingInertnessRecommended pH range
InertSustain C18First choice with ultra high inertness and high durabilityL12, 3, 5, 101035014Yes★★★★★1 - 10
InertSustain AQ-C18First choice for high polar compoundsL1, L961.9, 3, 51035013Yes★★★★★1 - 10
InertSustainSwift C18First analysis with ultra high inertness and high durabilityL11.9, 3, 5202009Yes★★★★★1 - 10
InertSustain AX-C18Analysis of anionic highly polar compoundsL1, L783, 5202008Yes★★★★1 - 9
InertCore Plus C18Ideal for analyses requiring a high number of theoretical stagesL12.6920015Yes★★★★1 - 10
Inertsil ODS-HLUltra high retentivity, High-density bonding of C18 phaseL13, 51045023Yes★★★★★2 - 7.5
Inertsil ODS-4Ultra high inertness, High plate count, Medium retentivityL12, 3, 51045011Yes★★★★★2 - 7.5
Inertsil ODS-4VInertsil ODS-4 Validated columnL13, 51045011Yes★★★★★2 - 7.5
Inertsil ODS-3Strong retentivity, Lower column backpressure, Very inertL12, 3, 4, 5, 101045015Yes★★★★2 - 7.5
Inertsil ODS-3VInertsil ODS-3 Validated columnL13, 51045015Yes★★★★2 - 7.5
Inertsil ODS-SPWeak retentivity, for hydrophobic compoundsL13, 5104508.5Yes★★★★2 - 7.5
Inertsil ODS-PHigh steric selectivityL13, 51045029None★★★2 - 7.5
Inertsil ODS-EPA polar functional group embeddedL15104509None★★★★2 - 7.5
Inertsil WP300 C18Analysis of high moleculesL15301509Yes★★★★2 - 7.5
Inertsil ODS-2Ultra pure silica gel is usedL151532018.5Yes★★★★2 - 7.5
Inertsil ODSInertness 1st generationL15,101035014Yes★★2 - 7.5
InertSustain C8First choice with ultra high inertness and high durabilityL72, 3, 5103508Yes★★★★★1 - 10
InertSustainSwift C8High inertness and high durability C8 columnL71.9, 3, 5202006Yes★★★★★1 - 10
Inertsil C8-4Ultra high inertness, High plate count, Low retentivityL72, 3, 5104505Yes★★★★★2 - 7.5
Inertsil C8-3Strong retentivity, Lower column backpressure, Very inertL72, 3, 5, 10104509Yes★★★★2 - 7.5
Inertsil C8Ultra pure silica gel is usedL751532010.5Yes★★2 - 7.5
Inertsil C4Low retentivityL265153207.5Yes★★★★2 - 7.5
Inertsil WP300 C8Suitable for high moleculesL75301504Yes★★★★2 - 7.5
Inertsil WP300 C4 L265301503 ★★★★2 - 7.5
InertSustain PFPExtremely Strong retention of highly polar basic compounds.L433, 51035010Yes★★★★★2 - 7.5
InertSustain PhenylhexylStrong π-π interactions and hydrophobic interactionsL113, 5103509Yes★★★★★1 - 10
InertSustain PhenyExtremely strong π-π interactionsL112, 3, 51035010 ★★★★2 - 7.5
Inertsil Ph-3Strong π-π interactionsL112, 3, 5104509.5 ★★★2 - 7.5
Inertsil PhHigh inertness, Weak π-π interactionsL1151532010Yes★★★2 - 7.5
InertSustain CyanoUltra inertness and can be used in reversed phase modeL103, 5103508Yes★★★★2 - 7.5
 

Comparison of Phases

 

The figure below shows the strength of the stereoselectivity and the pi-interaction with respect to the holding strength of the reverse phase column. The retention strength, stereoselection system, and strength of pi-interaction are based on the retention coefficient of amylbenzene, retention ratio of o-terphenyl and triphenylene, and retention ratio of amylbenzene and triphenylene, respectively. These are plotted below. In addition, the polar group-encapsulating (embed) type column provides a large effect on other interactions; thus, it was plotted in the type in order to distinguish it from the other columns.

 
Comparison of stereoselectivity between GL Sciences reversed phase columns
 
Strength of pi electron interaction between GL Sciences reversed phase columns
 

Selectivity Comparison of Reversed Phase Columns with Sample Analytes

 

Selectivity of reversed-phase columns are compared under the same conditions, packing material size and column size. Chemical structural formulas used for the comparison are shown at right. Separation differences between basic compounds, acidic compounds, alkyl benzenes and polyaromatic compounds shows the differences of column selectivity. The more silanol groups on the packing material lead the later elution time of caffeine compared with that of phenol. The higher hydrophobicity of the column shows the later elution time of n-Amylbenzene compared with that of n-Butylbenzene. The higher steric selectivity of the column shows the later elution time of Triphenylene compared with that of o-Terphenyl.

 

Conditions

 
ColumnReversed Phase Column (5 µm, 250 × 4.6 mm I.D.)*
EluentA) CH3OH
B) H2O
A/B = 80/20, v/v , (Inertsil Diol, CN-3)
A/B = 70/30, v/v ,
Flow Rate1.0 mL/min*
Col. Temp.40 °C
DetectionUV 254 nm
Injection Vol.5 µL*

* For Mono Clad C18-HS (3.0 mm I.D.), 0.4 mL/min of flow rate and 2 µL of injection volume are adopted according to the column internal diameter.

Analyte structures and labels
 
 
Set of Spectra for GL Sciences Reverse phase columns
 
Set of Spectra for GL Sciences Reverse phase columns 2
 
 

Reversed Phase Column Selection

 
For Molecular Weight <5,000
 
Reversed phase column selection for molecular weight less than 5000
 
For Molecular Weight >5,000
 
Reversed phase column selection for molecular weight more than 5000
 
For Highly Polar Compounds
 
Reversed phase column selection for highly polar compounds
 
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