Tailing of peaks or adsorption of peaks can be experienced when a column has residual adsorption sites. Such phenomenon would not be observed on InertSustain as the silanols are completely end-capped, having a neutral silica surface.
As shown below, core-shell columns show peak tailing due to the presence of trace metals or silanol groups in their silica gel. Quantitative and qualitative analysis will be a source of concern since the adsorption of compounds can be expected.
As shown in the experiment below, due to the introduction of Evolved Surface Silica, InertSustain C18 columns maintained high efficiency and peak shape for 300 hours while other “wide pH” column brands failed.
Highly end-capped ODS columns such as InertSustain C18 columns offer an opportunity to flush out contaminants from the column surface easily using an organic solvent. Coffee melanoidins are brown heterogeneous polymers contained in coffee. Its components are not clarified yet, but it is considered to contain several ionic compounds, which a poorly end-capped column will adsorb those ionic compounds leading to short column lifetime.
As for ODS column, which is commonly used for HPLC and LC/MS/MS, its inertness has an influence not only on peak shape but also detection sensitivity and durability. It is highly recommended to use highly end-capped column which provides good peak shape for both basic and acidic compounds such as InertSustain C18.
Rigorous quality control of physical properties and strict chromatographic tests for inertness and selectivity, contribute to the production of InertSustain C18 columns with outstanding reproducibility and long column lifetime.
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