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InertSustain AX-C18 is a reversed-phase mixed-mode column with an octadecyl group and an alkylamine group.
It is possible to retain polar acidic components that are generally not retained without the use of ion-pair reagents, and by setting the ion exchange capacity weaker, the buffer mobile phase used in LC / MS can be sufficiently eluted. Is designed for. It also has excellent inter-lot reproducibility, which tends to be a problem with columns.
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Specifications:
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Chemical bond group silica gel: High-purity spherical silica gel (ES silica)
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Particle size: 3µm, 5µm
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Surface area: 200m 2 / g
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Pore size: 200Å
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Pore volume: 1.00 mL / g
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Chemical bond group: Octadecyl group + tertiary amino group
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End-capping: Yes
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Carbon Loading: 8%
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USP Code: L1, L78
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Recommended PH: 1 to 9
Recommended when
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You want to slightly change the separation pattern from C18 columns
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You want to analyze acidic compounds and anionic compounds
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You are having trouble with reproducibility and lot-to-lot variation in anion exchange columns
Feature 1: Retention behavior that combines both reversed-phase and anion exchange
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Compared to typical C18 columns ➤➤➤Increased retention of acidic compounds
Analysis of nucleobases and monophosphates
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Conditions:
Column: 3 μm, 150 × 2.1 mm I.D.
Eluent: 25 mM NaH2PO4 in H2O
Flow Rate: 0.2 mL/min
Col. Temp.: 40 ℃
Detection： UV 254 nm
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Feature 2: Excellent retention for acidic compounds
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No ion paid reagent in eluent➤➤➤ Highly polar acidic compounds retained
Analysis of sugar‐nucleotides
Eluent ： 20 mM HCOONH4 in H2O
(Ion pair not used)
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Conditions
Column： 3 μm, 150 × 2.1 mm I.D.
Flow Rate： 0.2 mL/min
Col. Temp.： 40 ℃
Detection： UV 254 nm
Sample： 1. UDP‐Glu 2. UDP‐Gal 3. GDP‐Man
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Strongly retains highly polar acidic compounds which cannot normally be retained by C18 column for an analysis of highly polar compounds!
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Even if the salt concentration of the eluent is low ➤➤➤Acidic compounds strongly retained & Compatible with MS analysis
Analysis of monophosphoric acid
Eluent ：
A) 10 mM HCOONH4 in CH3OH
B) 10 mM HCOONH4 in H2O
A/B = 5/95 ‐ 10 min ‐ 100/0 ‐ 5 min ‐ 100/0, v/v
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Conditions
Column：3 μm, 150 × 2.1 mm I.D.
Flow Rate：0.2 mL/min
Col. Temp.：40 ℃
Inj. Volume：5 μL
Detection：MS/MS (ESI, Positive, MRM)
Sample: Q1 > Q3
1. CMP: 324.90 > 111.95 (+) 0.5 mg/L
2. GMP: 364.90 > 153.00 (+) 0.5 mg/L
3. UMP: 325.90 > 96.90 (+) 1.0 mg/L
4. AMP: 348.90 > 136.95 (+) 0.05 mg/L
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Strongly retains highly polar acidic compounds even when the salt concentration is as low as 10 mM! Sharp elution peaks! Can be analyzed at low salt concentration. Compatibility with MS!
Feature 3: Excellent reproducibility and quality
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Reproducibility issues with mixed-mode ➤➤➤ are solved by GL Sciences’ original packing and manufacturing technology
Shipping inspection for evaluation of lot‐to‐lot variation
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Conditions
Column ： 3 μm, 150 × 4.6 mm I.D.
Eluent ： CH3CN/H2O = 65/35, v/v
Flow Rate ： 1.0 mL/min
Col. Temp.：40 ℃
Detection ： UV 254 nm
Sample ： 1. Uracil 2. Acetophenone 3. Benzene 4. Toluene 5. Naphthalene
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Evaluation of lot‐to‐lot variation in acidic compounds
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Conditions
Column ： 3 μm, 150 × 4.6 mm I.D.
Eluent ： CH3CN/0.1 % HCOOH = 10/90, v/v
Flow Rate ： 1.0 mL/min
Col. Temp. ： 40 ℃
Detection ： UV 254 nm
Sample ： 1. AMP
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Excellent reproducibility of retention time in mixed‐mode columns where reproducibility is difficult to control!
COLUMNS LINEUP
InertSustain AX-C18 Analytical Columns
InertSustain AX‐C18 Cartridge Guard Column E (Pressure Resistance: 20 MPa)
Guard Column for UHPLC (Max. Operating Pressure: 80 MPa)
High-Pressure Resistant UHPLC PEEK Column (Max. Operating Pressure: 80 MPa)
