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Inertsil Hybrid-Si HILIC

Picture of Inertsil Hybrid-Si HILIC

Unbonded hybrid-silica L3 column with excellent alkaline durability up to pH 10. A new option for HILIC and normal-phase methods requiring robust silica-type selectivity.

  • High-pH Stability
  • HILIC Selectivity
  • LC/MS Compatibility
More about Inertsil Hybrid-Si HILIC

Specifications

Base Material: High-pH Stable Hybrid Silica
Particle size: 1.7 μm, 3.5 µm, 5 µm
Pore size: 165 Å (16.5 nm)
Pore volume: 1.00 mL/g
Surface area: 185 m²/g
Max Temperature: 80°C (pH 1 - 9), 60°C (pH 10)
pH Range: 1-10
USP Code: L3
Functional group: None
Mode: HILIC
Max pressure: 124 MPa (18,000 psi), 50 MPa (7,200 psi), 40 MPa (6,000 psi)
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About Inertsil Hybrid-Si HILIC

Designed to retain and resolve highly polar compounds that are difficult to analyze by reversed-phase chromatography.

Inertsil Hybrid HILIC uses fully crosslinked organic-inorganic hybrid silica. The base material combines the mechanical strength of silica with enhanced chemical stability, providing greater flexibility in method-development under HILIC conditions and, for Hybrid-Si HILIC, normal-phase conditions.

Alkaline Durability of Inertsil Hybrid-Si HILIC at pH 10 and 80 °C

 

Alkaline Durability of Inertsil Hybrid-Si HILIC at pH 10 and 80 °C. Relative Theoretical Plate Count (%) vs Time (h): Inertsil Hybrid-Si HILIC, Brand A (Si), Brand D-2 (Si), Brand B (Si)
 

Test conditions: 5 µm, 250 x 4.6 mm I.D.; 54 mM TEA (pH 10.0) / CH3CN = 70/30; 0.5 mL/min; 80 °C.

Analysis: 100 mM ammonium acetate / CH3CN = 10/90; UV 254 nm; nicotinamide and cytosine.

*Accelerated stress test; not recommended as routine operating conditions.

Application Examples

4 application examples

USP Flucytosine Compounded Oral Suspension

USP Flucytosine Compounded Oral Suspension. Flucytosine, RT: 2.7 min, Tf: 1.38

Conditions

ColumnInertsil Hybrid-Si HILIC
5 µm, 200 x 4.6 mm I.D.
EluentA) 13 mM CH3COONH4 + 0.1 % Diisopropylamine (pH7.5)
B) CH3CN
A/B = 50/50, v/v
Flow Rate1.0 mL/min
Col. Temp.30 °C
DetectionUV 280 nm
Inj. Vol.20 µL
AnalyteFlucytosine, 50 mg/L

USP Maprotiline Hydrochloride

USP Maprotiline Hydrochloride. Maprotiline, RT: 8.24 min, Tf: 1.2

Conditions

ColumnInertsil Hybrid-Si HILIC
5 µm, 250 x 4.6 mm I.D.
EluentA) 38 mM CH3COONH4 (pH8.3)
B) 2-Propanol
C) CH3OH
A/B/C = 20/15/65, v/v/v
Flow Rate1.0 mL/min
Col. Temp.40 °C
DetectionUV 272 nm
Inj. Vol.20 µL
AnalyteMaprotiline, 1000 mg/L

USP Hydrocortisone

USP Hydrocortisone. Hydrocortisone, RT: 7.1 min, Tf: 1.3

Conditions

ColumnInertsil Hybrid-Si HILIC
3.5 µm, 150 x 4.6 mm I.D.
EluentA) Butyl chloride
B) Tetrahydrofuran
C) CH3OH
D) CH3COOH
E) H2O
A/B/C/D/E = 890/56/28/24/0.4, v/v/v/v/v
Flow Rate1.5 mL/min
Col. Temp.30 °C
DetectionUV 254 nm
Inj. Vol.5 µL
AnalyteHydrocortisone, 40 mg/L

USP Cloprostenol

USP Cloprostenol. Cloprostenol, RT: 10.4 min, Tf: 1.0

Conditions

ColumnInertsil Hybrid-Si HILIC
5 µm, 250 x 4.6 mm I.D.
EluentA) Hexane
B) C2H5OH
C) CH3COOH
A/B/C = 90/10/1, v/v
Flow Rate1.8 mL/min
Col. Temp.40 °C
DetectionUV 220 nm
Inj. Vol.5 µL
AnalyteCloprostenol, 800 mg/L
No application example lists this compound.
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