Search
You have no items in your shopping cart.
Contact Us
Catalogs
Quick Order

Inertsil Hybrid-C18

Inertsil Hybrid-C18
Inertsil Hybrid-C18
Inertsil Hybrid-C18 was newly developed to meet the evolving needs of users, offering exceptional durability under high-pH mobile phase conditions. This column expands application flexibility while maintaining the trusted performance of the Inertsil series.
Specifications
  • Base Material: High-pH Stable Hybrid Silica
  • Particle size: 1.7 μm, 3.5 μm, 5 µm
  • Carbon loading: 15.0%
  • End capping: Yes
  • Pore size: 165 Å (16.5 nm)
  • USP Code: L1
  • Pore volume: 1.00 mL/g
  • Max Temperature: 80°C (pH 1 - 10), 60°C (pH 1 - 12)
  • Surface area: 185 m²/g
  • pH Range: 1-12
×

Inertsil Hybrid-C18

View Product List ( Items)
Start Over
×

Inertsil Hybrid-C18 ( Items)

Item NumberPriceAction
Cancel Edit Specifications
  • Developing a new method?

    Get free expert guidance on column selection and method optimization.

  • Request information or Quote

    Have a product question or need a format quote?
    Contact us now!

GL Sciences: Trusted by Scientists Since 1968

About Inertsil Hybrid-C18

 

A Legacy of Quality

 

Since the launch of Inertsil ODS-3 in 1994, we have consistently manufactured HPLC columns in-house—from the synthesis of base silica to final packing. This fully integrated production process is the foundation of our ability to deliver high-quality, highly reliable columns with unmatched consistency. 

Inertsil Hybrid-C18 was newly developed to meet the evolving needs of users, offering exceptional durability under high-pH mobile phase conditions. This column expands application flexibility while maintaining the trusted performance of the Inertsil series.

 
 
 

One Column, Infinite Possibilities

Impact of Mobile Phase pH on Selectivity

 

These chromatograms demonstrate how changes in mobile phase pH influence the selectivity and resolution of analytes. Inertsil Hybrid-C18 of hybrid silica gel allows stable performance across this wide pH range without loss of efficiency and reproducibility

 
 

  • Basic compounds (pKa ≈ 9) show stronger retention at high pH due to reduced ionization

  • Acidic compounds (e.g., COOH) retain better at low pH where ionization is suppressed.

  • Understanding pKa enables control over separation behavior.

 
 
 
 

Alkali-Resistant Hybrid Silica Technology

 

    Superior Durability Under High pH Conditions (pH 11.5)

     

    With its advanced hybrid silica technology, Inertsil Hybrid-C18 delivers exceptional resistance to alkaline mobile phase condition. Even under high-pH mobile phase conditions that typically cause dissolution of conventional packings, the column maintains its structural integrity.

    This unique robustness prevents loss of theoretical plates, guaranteeing reliable peak resolution, reproducible separations, and extended column lifetime.

     

    Mobile Phase for Durability Testing

    Column : 5 µm, 150 × 2.1 mm I.D.
    Eluent : A) 50 mM Phosphate Buffer (pH 11.5) in H2O
        B) CH3CN
    A/B = 90/10, v/v
    Flow Rate : 0.2 mL/min
    Col. Temp. : 40 °C

    Mobile Phase for Analysis

    Eluent : A) H2O
        B) CH3CN
    A/B = 35/65, v/v
    Flow Rate : 0.2 mL/min
    Col. Temp. : 40 °C
    Detection : 254 nm
    Sample : Naphthalene

     
     

    Consistent Theoretical Plate Count at High pH

    Key features visual
     
     

    Excellent batch-to-batch reproducibility

    Comparison of three different production lots of Inertsil Hybrid-C18 show virtually identical retention and peak shape, demonstrating outstanding lot-to-lot consistency. This high reproducibility ensures method robustness and eliminates the need for revalidation when switching lots.

     
     
     

    Superior Performance Across Challenging Analytes with Inertsil Hybrid-C18

    Inertsil Hybrid-C18 demonstrates excellent peak shape for basic compounds, acidic compounds, and metal-coordinating compounds, outperforming competitor columns.This high-level performance is achieved through our proprietary hybrid silica technology, which offers superior resistance to peak tailing and adsorption issues under a wide range of pH conditions.

     
     
     

    Column Hardware Lineup

     

      Wide Range of Dimensions and Hardware Options

       

      Hardware is available in two choices: stainless steel (SS) and biocompatible PEEK, making it suitable for everything from routine analyses to LC/MS, LC/MS/ MS, and sensitive biological applications.

      Customized Dimensions Available on Request

      In addition to our standard lineup, custom column dimensions and configurations can be made to order, including preparative-scale dimensions and specialty hardware options.

       
       
      Key features visual
       
       

      Applications

       
       

      Guanine-based antiviral agent

       
      Chromatogram - Guanine-based antiviral agent
       

      Conditions
      Column : 5 µm, 150 × 2.1 mm I.D.
      Eluent : 10 mM HCOONH4 in H2O
      Flow Rate : 0.3 mL/min
      Col. Temp. : 40 °C
      Detection : UV 254 nm
      Inj. Vol. : 1 µL
      Analytes : 1. Ganciclovir  2. Aciclovir  3. Penciclovir (100 mg/L each)

       
       

      Nucleoside-based antiviral drug

       
      Chromatogram - Nucleoside-based antiviral drug
       

      Conditions
      Column : 3.5 µm, 150 × 2.1 mm I.D.
      Eluent : 10 mM HCOONH4 in H2O
      Flow Rate : 0.3 mL/min
      Col. Temp. : 40 °C
      Detection : UV 240 nm
      Inj. Vol. : 1 µL
      Analytes : 1. Ribavirin  2. Cytarabine  3. Gemcitabine  4. Lamivudine  5. Vidarabine (100 mg/L each)

       
       

      Azithromycin Tablet of Assay under USP condition

       
      Chromatogram — Azithromycin Tablet Assay (USP)
       

      Conditions
      Column : 5 µm, 250 × 4.6 mm I.D.
      Eluent : CH3CN/Buffer* = 65/35, v/v
      Flow Rate : 2.0 mL/min
      Col. Temp. : 50 °C
      Detection : UV 210 nm
      Inj. Vol. : 100 µL
      Analyte : Reference Standard in Eluent — 1. Azithromycin dihydrate 1 mg/mL

      *Dissolve 4.6 g of monobasic potassium phosphate anhydrous in 900 mL of water. Adjust with 1 N sodium hydroxide to pH 7.5, and dilute with water to 1 L.

       
       

      Azithromycin Tablet of Dissolution under USP condition

       
      Chromatogram — Azithromycin Tablet Dissolution (USP)
       

      Conditions
      Column : 5 µm, 150 × 4.6 mm I.D.
      Eluent : CH3CN/CH3OH/Solution* = 9/3/8, v/v/v
      Flow Rate : 1.5 mL/min
      Col. Temp. : 50 °C
      Detection : UV 210 nm
      Inj. Vol. : 50 µL
      Sample : Reference Standard — 1. Azithromycin dihydrate 0.25 mg/mL

      *4.4 mg/mL of dibasic potassium phosphate and 0.5 mg/mL of sodium 1-octanesulfonate; adjusted with phosphoric acid to a pH of 8.20 ± 0.05.

       
       

      Antihistamine

       
      Chromatogram — Antihistamine
       

      Conditions
      Column : 3.5 µm, 150 × 2.1 mm I.D.
      Eluent : A) 10 mM HCOONH4 in H2O   B) CH3CN
      Program : A/B = 80/20 – 15 min – 20/80 – 0.1 min – 80/20, v/v
      Flow Rate : 0.3 mL/min
      Col. Temp. : 40 °C
      Detection : UV 240 nm
      Inj. Vol. : 2 µL
      Analytes : 1. Epinastine  2. Chlorpheniramine  3. Fexofenadine  4. Triprolidine  5. Ketotifen  7. Azelastine  8. Hydroxyzine  9. Homochlorcyclizine  10. Clemastine  11. Loratadine
      (50 mg/L each)

       
       

      Antidepressant

       
      Chromatogram — Antidepressant
       

      Conditions
      Column : 3.5 µm, 150 × 2.1 mm I.D.
      Eluent : A) 10 mM HCOONH4 in H2O   B) CH3CN
      Mix : A/B = 50/50, v/v
      Flow Rate : 0.3 mL/min
      Col. Temp. : 40 °C
      Detection : UV 230 nm
      Inj. Vol. : 1 µL
      Analytes : 1. Nortriptyline  2. Imipramine  3. Amitriptyline  4. Sertraline  5. Clomipramine  6. Mianseline
      (100 mg/L each)

       
      Show More
      Documents
      ×

      Request Info / Quote

      Need expert advice or a fast quote?

      Call 1-310-984-1946 or Send Message Now!


      *
      *
      *
      By clicking Send, you agree to our Privacy Policy & Terms of Service
        Contact Info
        Information
        Customer Service
        Copyright © 2025 GL Sciences Inc.. All rights reserved.