InertSep Oligo SPE

Introduction of InertSep Oligo SPE Plate

Streamlined Workflow, Consistent Results

Oligonucleotide therapeutics have attracted increasing attention in recent years due to their high target specificity and low toxicity. For quantitative analysis, ligand-binding assays have been widely used and continue to play an important role due to their high sensitivity and throughput. 

However, LC/MS-based methods are becoming increasingly preferred in certain stages of development, as they offer enhanced selectivity and enable clear differentiation between the parent oligonucleotide and its metabolites. In these workflows, robust and reproducible sample preparation is essential to ensure reliable analytical results.

InertSep Oligo is a solid-phase extraction (SPE) product specifically designed for oligonucleotide purification in LC/MS workflows. It minimizes variability during the extraction process and enables high recovery of target analytes. Automation-compatible formats, including 96-well plates and microelution plates, are available— making it well-suited for high-throughput applications using automated liquid handling systems.

Format

InertSep Oligo is available in two formats—96-well plates and cartridges— to flexibly support a wide range of sample preparation workflows, from high-throughput automated processing to manual small-scale operations.

The 96-well plate comes in two types: a 2 mL square-well format that allows for larger sample volumes, and a μElution format that enables efficient extraction and concentration with minimal solvent. Both types are highly compatible with LC/MS/MS, ideal for high-sensitivity nucleic acid analysis. They also offer excellent compatibility with automated systems, making them well-suited for high-throughput nucleic acid extraction.

All formats use the same sorbent, ensuring full compatibility with LC/MS workflows and delivering consistent performance and reliability across different scales and instrument platforms.