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HILIC HPLC Columns

GL Sciences HILIC columns are designed for reliable separation of highly polar compounds using hydrophilic interaction chromatography. With bonded phases such as amide and amino — including InertSustain Amide, InertSustain NH₂, Inertsil HILIC, and ProteoSil HILIC — these columns deliver strong retention, sharp peak shape, and reproducibility for analytes like sugars, glycans, polar bases, and water-soluble compounds. Compatible with LC-MS workflows and buffers such as ammonium formate or acetate, HILIC phases are available in multiple formats to support routine analysis and high-sensitivity workflows. 

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Product DetailsName⬍
Price⬍
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InertSustain Amide
L68
-
100 Å (10 nm)
350 m2/g
15.0%
None
View Options
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phases.
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Inertsil Amide HILIC HPLC Columns
Inertsil Amide
L68
-
100 Å (10 nm)
450 m2/g
18.0%
None
View Options
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime.
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Inertsil HILIC
L20
-
100 Å (10 nm)
450 m2/g
20.0%
None
View Options
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic solvent concentration is ...
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InertSustain NH2
InertSustain NH2
L8
-
100 Å (10 nm)
350 m2/g
7.0%
None
View Options
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of sugars or vitamin E. However, the shift ...
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Inertsil NH2
L8
-
100 Å (10 nm)
450 m2/g
8.0%
None
View Options
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of sugars or vitamin E. However, ...
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ProteoSil HILIC
L68
-
100 Å (10 nm)
-
-
-
View Options
Modified with amide groups, the ProteoSil HILIC column excels in the separation of highly hydrophilic compounds, peptides, glycans, and oligonucleotides.
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InertSustain Amide HPLC Column, 1.9 µm, 50 x 3.0 mm
Item No. 5020-88821
L68
1.9 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$869.00 / 1 pc.
More Details
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Inertsil NH2 Semi-Prep HPLC Column, 5 µm, 100 x 6.0 mm
Item No. 5020-05554
L8
5 µm
100 Å (10 nm)
450 m2/g
8.0%
None
$869.00 / 1 pc.
More Details
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Inertsil HILIC Capillary EX-Nano Column, 3 µm, 50 x 0.075 mm
Item No. 5020-15175
L20
3 µm
100 Å (10 nm)
450 m2/g
20.0%
None
$1,109.00 / 1 pc.
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Inertsil HILIC Capillary EX-Nano Column, 3 µm, 150 x 0.05 mm
Item No. 5020-15075
L20
3 µm
100 Å (10 nm)
450 m2/g
20.0%
None
$1,413.00 / 1 pc.
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Inertsil HILIC Capillary EX-Nano Column, 3 µm, 50 x 0.05 mm
Item No. 5020-15025
L20
3 µm
100 Å (10 nm)
450 m2/g
20.0%
None
$1,109.00 / 1 pc.
More Details
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InertSustain Amide HPLC Column, InertCoat Hardware, 3 µm, 100 x 2.1 mm
Item No. 5020-32023
L68
3 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,130.00 / 1 pc.
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Inertsil NH2 HPLC Column, 10 µm, 150 x 3.9 mm
Item No. 5020-90654
L8
10 µm
100 Å (10 nm)
450 m2/g
8.0%
None
$942.00 / 1 pc.
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InertSustain Amide HPLC Column, InertCoat Hardware, 1.9 µm, 50 x 2.1 mm
Item No. 5020-32019
L68
1.9 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,130.00 / 1 pc.
More Details
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InertSustain Amide HPLC Column, InertCoat Hardware, 1.9 µm, 150 x 2.1 mm
Item No. 5020-32021
L68
1.9 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,339.00 / 1 pc.
More Details
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InertSustain Amide HPLC Column, InertCoat Hardware, 3 µm, 150 x 2.1 mm
Item No. 5020-32024
L68
3 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,193.00 / 1 pc.
More Details
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InertSustain NH2 HPLC Column, 5 µm, 150 x 3.0 mm
Item No. 5020-90653
L8
5 µm
100 Å (10 nm)
350 m2/g
7.0%
None
$774.00 / 1 pc.
More Details
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InertSustain Amide HPLC Column, InertCoat Hardware, 1.9 µm, 100 x 2.1 mm
Item No. 5020-32020
L68
1.9 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,203.00 / 1 pc.
More Details
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InertSustain Amide HPLC Column, InertCoat Hardware, 3 µm, 50 x 2.1 mm
Item No. 5020-32022
L68
3 µm
100 Å (10 nm)
350 m2/g
15.0%
None
$1,078.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 100 x 4.6 mm
Item No. 5020-42175
-
5 µm
100 Å (10 nm)
-
-
-
$1,663.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 3 µm, 250 x 4.6 mm
Item No. 5020-42167
-
3 µm
100 Å (10 nm)
-
-
-
$2,165.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 1.9 µm, 75 x 2.1 mm
Item No. 5020-42238
-
1.9 µm
100 Å (10 nm)
-
-
-
$1,172.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 3 µm, 250 x 2.1 mm
Item No. 5020-42237
-
3 µm
100 Å (10 nm)
-
-
-
$1,140.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 250 x 4.6 mm
Item No. 5020-42177
-
5 µm
100 Å (10 nm)
-
-
-
$1,862.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 50 x 4.6 mm
Item No. 5020-42174
-
5 µm
100 Å (10 nm)
-
-
-
$1,318.00 / 1 pc.
More Details
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ProteoSil HILIC UHPLC PEEK HPLC Column, 3 µm, 100 x 4.6 mm
Item No. 5020-42165
-
3 µm
100 Å (10 nm)
-
-
-
$1,893.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 3 µm, 150 x 2.1 mm
Item No. 5020-42236
-
3 µm
100 Å (10 nm)
-
-
-
$994.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 250 x 2.1 mm
Item No. 5020-42224
-
5 µm
100 Å (10 nm)
-
-
-
$1,015.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 150 x 2.1 mm
Item No. 5020-42172
-
5 µm
100 Å (10 nm)
-
-
-
$1,663.00 / 1 pc.
More Details
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ProteoSil HILIC UHPLC PEEK HPLC Column, 3 µm, 150 x 4.6 mm
Item No. 5020-42166
-
3 µm
100 Å (10 nm)
-
-
-
$1,935.00 / 1 pc.
More Details
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ProteoSil HILIC, UHPLC PEEK HPLC Column, 1.9 µm, 100 x 2.1 mm
Item No. 5020-42243
-
1.9 µm
100 Å (10 nm)
-
-
-
$2,070.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 75 x 4.6 mm
Item No. 5020-42226
-
5 µm
100 Å (10 nm)
-
-
-
$795.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 100 x 2.1 mm
Item No. 5020-42171
-
5 µm
100 Å (10 nm)
-
-
-
$1,663.00 / 1 pc.
More Details
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ProteoSil HILIC UHPLC PEEK HPLC Column, 3 µm, 100 x 2.1 mm
Item No. 5020-42161
-
3 µm
100 Å (10 nm)
-
-
-
$1,893.00 / 1 pc.
More Details
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ProteoSil HILIC, UHPLC PEEK HPLC Column, 1.9 µm, 50 x 2.1 mm
Item No. 5020-42242
-
1.9 µm
100 Å (10 nm)
-
-
-
$1,998.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 1.9 µm, 150 x 2.1 mm
Item No. 5020-42240
-
1.9 µm
100 Å (10 nm)
-
-
-
$1,339.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 150 x 2.1 mm
Item No. 5020-42223
-
5 µm
100 Å (10 nm)
-
-
-
$879.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 50 x 4.6 mm
Item No. 5020-42225
-
5 µm
100 Å (10 nm)
-
-
-
$733.00 / 1 pc.
More Details
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ProteoSil HILIC UHPLC PEEK HPLC Column, 3 µm, 250 x 2.1 mm
Item No. 5020-42163
-
3 µm
100 Å (10 nm)
-
-
-
$2,165.00 / 1 pc.
More Details
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ProteoSil HILIC UHPLC PEEK HPLC Column, 3 µm, 50 x 2.1 mm
Item No. 5020-42160
-
3 µm
100 Å (10 nm)
-
-
-
$1,820.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 150 x 4.6 mm
Item No. 5020-42176
-
5 µm
100 Å (10 nm)
-
-
-
$1,663.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 50 x 2.1 mm
Item No. 5020-42170
-
5 µm
100 Å (10 nm)
-
-
-
$1,318.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 3 µm, 75 x 2.1 mm
Item No. 5020-42234
-
3 µm
100 Å (10 nm)
-
-
-
$869.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 150 x 4.6 mm
Item No. 5020-42228
-
5 µm
100 Å (10 nm)
-
-
-
$879.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 75 x 2.1 mm
Item No. 5020-42221
-
5 µm
100 Å (10 nm)
-
-
-
$795.00 / 1 pc.
More Details
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ProteoSil HILIC PEEK HPLC Column, 5 µm, 250 x 2.1 mm
Item No. 5020-42173
-
5 µm
100 Å (10 nm)
-
-
-
$1,862.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 5 µm, 250 x 4.6 mm
Item No. 5020-42229
-
5 µm
100 Å (10 nm)
-
-
-
$1,015.00 / 1 pc.
More Details
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ProteoSil HILIC, UHPLC PEEK HPLC Column, 3 µm, 50 x 4.6 mm
Item No. 5020-42164
-
3 µm
100 Å (10 nm)
-
-
-
$1,820.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 3 µm, 100 x 2.1 mm
Item No. 5020-42235
-
3 µm
100 Å (10 nm)
-
-
-
$869.00 / 1 pc.
More Details
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ProteoSil HILIC HPLC Column, 3 µm, 50 x 2.1 mm
Item No. 5020-42233
-
3 µm
100 Å (10 nm)
-
-
-
$774.00 / 1 pc.
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