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HILIC Columns

HPLC columns for extraction by HILIC (Hydrophilic Interaction Chromatography) mode. GL Sciences HILIC columns are designed for the analysis of neutral and basic compounds providing excellent peak shape. A choice of phases are available for specificity towards target analytes, including some purpose-built columns such as for sugar analysis. Read more

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Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $207.00
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $207.00
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime.
From $207.00
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $207.00
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $207.00
SKU# 5020-07742
$485.00
SKU# 5020-07752
$646.00
SKU# 5020-86752
$859.00
SKU# 5020-86762
$1,321.00
SKU# 5020-86772
$2,679.00
SKU# 5020-07744
$490.00
SKU# 5020-07745
$513.00
SKU# 5020-07746
$600.00
SKU# 5020-07756
$848.00
SKU# 5020-86756
$1,356.00
SKU# 5020-86766
$3,361.00
SKU# 5020-86776
$6,034.00
SKU# 5020-86786
$12,878.00
SKU# 5020-85554
$1,039.00
SKU# 5020-85555
$1,194.00
SKU# 5020-85556
$1,356.00
SKU# 5020-79046
$1,194.00
SKU# 5020-79047
$1,628.00
SKU# 5020-79048
$1,917.00

HILIC Column Specification


Columns

Features

USD Code

Particle Size (µm)

Pore Size (nm)

Surface Area (m2/g)

Carbon Loading (%)

Recommended pH range

InertSustain Amide

First choice column for HILIC mode.

L68

3, 5

10

350

15

2 - 8.5

Inertsil Amide

Effective when the retention of high polar compounds is further strengthened.

L68

3, 5

10

450

18

2 - 7.5

Inertsil HILIC

Effective when the overall retention is to be reduced or when the separation pattern is to be changed.

L20

3, 5

10

450

20

2 - 7.5

InertSustain NH2

First choice column for sugar analysis.

L8

3, 5

10

350

7

2 - 7.5

Inertsil NH2

Effective for intensifying retention in sugar analysis

L8

3, 5

10

450

8

2 - 7.5

 

HILIC Mode Chromatography

As an alternative to reversed-phase chromatography, Hydrophilic Interaction Chromatography (HILIC) was developed for analyses of highly polar compounds. The retention in HILIC mode usually increases with increasing concentration of an organic compound. Moreover, the stability is improved by adding a basic solvent (such as ammonium acetate) to the eluent.

 

Comparison of Separation Mode between Reversed Phase and HILIC Columns

Comparison of Separation Mode between Reversed Phase and HILIC Columns
 

 Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase

Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase
 

HILIC Column Selection

HILIC Column Selection flowchart
 

HILIC Columns Resources:​

  • Download GL Sciences 2022 HILIC Catalog —  Download & Discover GL Sciences' HILIC Columns
  • Search Application Notes For HILIC Columns — Search  Applications Database
  • HPLC HILIC Columns’ Specifications and Selection Guide — Summary of GL Sciences HILIC Columns Specifications and guide to select. Learn More
  • HPLC Column Selection By USP  — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
  • HPLC Column End-fittings  — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
  • How to Select a Guard Column  — Download guide to select the right guard column. Learn More
  • Choosing Preparative Column  — Download Guide to select the right preparative column. Learn More
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