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HILIC Columns

GL Sciences provides amide, amine and diol phases column phases for separation by HPLC using HILIC (Hydrophilic Interaction Chromatography) mode. GL Sciences HILIC phases feature several generations of development of evolved silica for the analysis of neutral and basic compounds with excellent peak shape. Specific applications of GL Sciences HILIC phases are analysis of sugars and vitamins. Read more

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InertSustain Amide HILIC HPLC Columns
InertSustain Amide
InertSustain Amide column is a HILIC (Hydrophilic Interaction Chromatography) column for enhanced retention of extremely polar compounds. It offers the strongest retentivity among the Amide columns available in the market due to the usage and bonding of carbamoyl groups. Superior stability and durability even under water rich mobile phase...
From $280.00
Inertsil Amide HILIC HPLC Columns
Inertsil Amide
Inertsil Amide column is bonded of carbamoyl group, and it shows strong retention of highly polar compounds. As shown below, compared to other commercial columns, Inertsil Amide column showed high retentivity. Inertsil Amide column provides excellent performance for those hard to retain compounds using an ODS column with a long lifetime.
From $280.00
Inertsil HILIC
Inertsil HILIC
HILIC is, abbreviated name for Hydrophilic Interaction Chromatography, and it was developed as an alternative to reversed phase chromatography for highly polar compounds. Inertsil HILIC is the column which chemically bonded with diol group, and it provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when...
From $280.00
InertSustain NH2
InertSustain NH2
InertSustain NH2 shows far superior stability compared to other brand available aminopropyl columns, as our newly developed “Evolved Surface Silica” is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous analysis of ...
From $280.00
Inertsil NH2 HILIC Amino HPLC Columns
Inertsil NH2
Inertsil NH2 amino column shows far superior stability compared to other brand available aminopropyl columns, as our newly developed "Evolved Surface Silica" is chemically bonded with aminopropyl group. Generally, aminopropyl columns are used for applications that are hard to be separated in a reversed phase mode, such as simultaneous ana...
From $280.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 4.6 mm (analytical)
SKU# 5020-07835
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 4.6 mm (analytical)
SKU# 5020-07836
$640.00
Inertsil HILIC Prep Column, 5 µm, 50 x 6.0 mm
SKU# 5020-07742
$630.00
Picture of Inertsil HILIC Prep Column, 5 µm, 50 x 7.6 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 7.6 mm
SKU# 5020-07752
$810.00
Imagen de Inertsil HILIC Prep Column, 5 µm, 50 x 10 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 10 mm
SKU# 5020-86752
$1,140.00
Picture of Inertsil HILIC Prep Column, 5 µm, 50 x 20 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 20 mm
SKU# 5020-86762
$1,980.00
Picture of Inertsil HILIC Prep Column, 5 µm, 50 x 30 mm
Inertsil HILIC Prep Column, 5 µm, 50 x 30 mm
SKU# 5020-86772
$3,330.00
Picture of Inertsil HILIC Prep Column, 5 µm, 100 x 6.0 mm
Inertsil HILIC Prep Column, 5 µm, 100 x 6.0 mm
SKU# 5020-07744
$690.00
Picture of Inertsil HILIC Prep Column, 5 µm, 150 x 6.0 mm
Inertsil HILIC Prep Column, 5 µm, 150 x 6.0 mm
SKU# 5020-07745
$740.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 6.0 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 6.0 mm
SKU# 5020-07746
$790.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 7.6 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 7.6 mm
SKU# 5020-07756
$1,140.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 10 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 10 mm
SKU# 5020-86756
$2,040.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 20 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 20 mm
SKU# 5020-86766
$3,950.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 30 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 30 mm
SKU# 5020-86776
$9,010.00
Picture of Inertsil HILIC Prep Column, 5 µm, 250 x 50 mm
Inertsil HILIC Prep Column, 5 µm, 250 x 50 mm
SKU# 5020-86786
$18,000.00
Picture of Inertsil Amide HPLC Column, 3 µm, 50 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 50 x 2.1 mm (analytical)
SKU# 5020-07862
$530.00
Picture of Inertsil Amide HPLC Column, 3 µm, 100 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 100 x 2.1 mm (analytical)
SKU# 5020-07864
$560.00
Picture of Inertsil Amide HPLC Column, 3 µm, 150 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 150 x 2.1 mm (analytical)
SKU# 5020-07865
$620.00
Picture of Inertsil Amide HPLC Column, 3 µm, 250 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 250 x 2.1 mm (analytical)
SKU# 5020-07866
$660.00
Picture of Inertsil Amide HPLC Column, 3 µm, 50 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 50 x 3.0 mm (analytical)
SKU# 5020-07872
$530.00
Picture of Inertsil Amide HPLC Column, 3 µm, 100 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 100 x 3.0 mm (analytical)
SKU# 5020-07874
$560.00
Picture of Inertsil Amide HPLC Column, 3 µm, 150 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 150 x 3.0 mm (analytical)
SKU# 5020-07875
$620.00
Picture of Inertsil Amide HPLC Column, 3 µm, 250 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 250 x 3.0 mm (analytical)
SKU# 5020-07876
$660.00
Picture of Inertsil Amide HPLC Column, 3 µm, 150 x 4.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 150 x 4.0 mm (analytical)
SKU# 5020-07885
$620.00
Picture of Inertsil Amide HPLC Column, 3 µm, 250 x 4.0 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 250 x 4.0 mm (analytical)
SKU# 5020-07886
$660.00
Picture of Inertsil Amide HPLC Column, 3 µm, 150 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 150 x 4.6 mm (analytical)
SKU# 5020-07895
$620.00
Picture of Inertsil Amide HPLC Column, 3 µm, 250 x 4.6 mm (analytical)
Inertsil Amide HPLC Column, 3 µm, 250 x 4.6 mm (analytical)
SKU# 5020-07896
$660.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 2.1 mm (analytical)
SKU# 5020-07805
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 2.1 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 2.1 mm (analytical)
SKU# 5020-07806
$640.00
Picture of Inertsil Amide HPLC Column, 5 µm, 150 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 150 x 3.0 mm (analytical)
SKU# 5020-07815
$600.00
Picture of Inertsil Amide HPLC Column, 5 µm, 250 x 3.0 mm (analytical)
Inertsil Amide HPLC Column, 5 µm, 250 x 3.0 mm (analytical)
SKU# 5020-07816
$640.00
5020-85554 Inertsil NH2 Prep Column, 5 µm, 100 x 10 mm
Inertsil NH2 Prep Column, 5 µm, 100 x 10 mm
SKU# 5020-85554
$1,460.00
5020-85555 Inertsil NH2 Prep Column, 5 µm, 150 x 10 mm
Inertsil NH2 Prep Column, 5 µm, 150 x 10 mm
SKU# 5020-85555
$1,750.00
5020-85556 Inertsil NH2 Prep Column, 5 µm, 250 x 10 mm
Inertsil NH2 Prep Column, 5 µm, 250 x 10 mm
SKU# 5020-85556
$2,040.00
5020-79046 Inertsil NH2 Prep Column, 5 µm, 50 x 14 mm
Inertsil NH2 Prep Column, 5 µm, 50 x 14 mm
SKU# 5020-79046
$1,450.00
5020-79047 Inertsil NH2 Prep Column, 5 µm, 100 x 14 mm
Inertsil NH2 Prep Column, 5 µm, 100 x 14 mm
SKU# 5020-79047
$2,310.00
5020-79048 Inertsil NH2 Prep Column, 5 µm, 150 x 14 mm
Inertsil NH2 Prep Column, 5 µm, 150 x 14 mm
SKU# 5020-79048
$2,500.00
5020-79049 Inertsil NH2 Prep Column, 5 µm, 250 x 14 mm
Inertsil NH2 Prep Column, 5 µm, 250 x 14 mm
SKU# 5020-79049
$2,890.00
5020-85562 Inertsil NH2 Prep Column, 5 µm, 50 x 20 mm
Inertsil NH2 Prep Column, 5 µm, 50 x 20 mm
SKU# 5020-85562
$1,980.00
5020-85564 Inertsil NH2 Prep Column, 5 µm, 100 x 20 mm
Inertsil NH2 Prep Column, 5 µm, 100 x 20 mm
SKU# 5020-85564
$3,330.00
5020-85565 Inertsil NH2 Prep Column, 5 µm, 150 x 20 mm
Inertsil NH2 Prep Column, 5 µm, 150 x 20 mm
SKU# 5020-85565
$3,760.00
5020-85566 Inertsil NH2 Prep Column, 5 µm, 250 x 20 mm
Inertsil NH2 Prep Column, 5 µm, 250 x 20 mm
SKU# 5020-85566
$3,950.00
5020-85572 Inertsil NH2 Prep Column, 5 µm, 50 x 30 mm
Inertsil NH2 Prep Column, 5 µm, 50 x 30 mm
SKU# 5020-85572
$3,330.00
5020-85576 Inertsil NH2 Prep Column, 5 µm, 250 x 30 mm
Inertsil NH2 Prep Column, 5 µm, 250 x 30 mm
SKU# 5020-85576
$9,010.00
5020-85586 Inertsil NH2 Prep Column, 5 µm, 250 x 50 mm
Inertsil NH2 Prep Column, 5 µm, 250 x 50 mm
SKU# 5020-85586
$18,000.00

HILIC Column Specification​


Columns

Features

USD Code

Particle Size (µm)

Pore Size (nm)

Surface Area (m2/g)

Carbon Loading (%)

Recommended pH range

InertSustain Amide

First choice column for HILIC mode.

L68

3, 5

10

350

15

2 - 8.5

Inertsil Amide

Effective when the retention of high polar compounds is further strengthened.

L68

3, 5

10

450

18

2 - 7.5

Inertsil HILIC

Effective when the overall retention is to be reduced or when the separation pattern is to be changed.

L20

3, 5

10

450

20

2 - 7.5

InertSustain NH2

First choice column for sugar analysis.

L8

3, 5

10

350

7

2 - 7.5

Inertsil NH2

Effective for intensifying retention in sugar analysis

L8

3, 5

10

450

8

2 - 7.5

 

HILIC Mode Chromatography

As an alternative to reversed-phase chromatography, Hydrophilic Interaction Chromatography (HILIC) was developed for analyses of highly polar compounds. The retention in HILIC mode usually increases with increasing concentration of an organic compound. Moreover, the stability is improved by adding a basic solvent (such as ammonium acetate) to the eluent.

 

Comparison of Separation Mode between Reversed Phase and HILIC Columns

Comparison of Separation Mode between Reversed Phase and HILIC Columns
 

 Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase

Correlation between Retention Time and the Concentration of CH3CN in Mobile Phase
 

HILIC Column Selection

HILIC Column Selection flowchart
 

HILIC Columns Resources:​

  • Download GL Sciences 2022 HILIC Catalog —  Download & Discover GL Sciences' HILIC Columns
  • Search Application Notes For HILIC Columns — Search  Applications Database
  • HPLC HILIC Columns’ Specifications and Selection Guide — Summary of GL Sciences HILIC Columns Specifications and guide to select. Learn More
  • HPLC Column Selection By USP  — Recommended GL Sciences HPLC Columns based on USP Code. Learn More
  • HPLC Column End-fittings  — Summary of GL Sciences' end-fitting style & recommended operating pressure. Learn More
  • How to Select a Guard Column  — Download guide to select the right guard column. Learn More
  • Choosing Preparative Column  — Download Guide to select the right preparative column. Learn More
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