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SPE Spin Centrifuge Columns
GL Sciences MonoSpin SPE spin columns provide improved concentration and yield for low-volume sample pre-treatment using centrifuge. MonoSpin features GL Sciences’ monolith silica technology with low-pressure, high-flow, and low-liquid-retention properties that allow for extraction with high recovery using smaller sample volumes compared to traditional particle media. MonoSpin columns are available in several formats for loading different sample volumes onto centrifuge. Read more
S Type spin column
L Type large volume reservoir spin column
96-well plate
- Disk size: 4.2 O.D. x 1.5 mm
- Sample volume: 50-800 µL
- Elution volume: 50-800 µL
- Centrifugation speed: 2,000-10,000 x g
- Disk size: 9 O.D. × 3 mm
- Sample volume: 0.5-8 mL
- Elution volume: 0.5-8 mL
- Centrifugation speed: 1,000 x g
- Disk size: 4.2 O.D × 1.5 mm
- Sample volume:～800 µL
- Elution volume: 100-800 µL
- Centrifugation speed: 2,000-10,000 x g (centrifuge; can also be used in vacuum aspiration)
Available Functional Groups for Spin Columns
How to choose Functional Groups
The MonoSpin series are optimized as a spin-column for pretreatment of biological samples. If you are working on highly viscous samples such as blood, MonoSpin C18 FF is the best choice. Please refer to the following chart for choosing appropriate MonoSpin:
MonoSpin Features
- Easy operation by centrifuge
- Speedy sample treatment with a superb through pore
- Excellent reproducibility (S-type) even at elution volumes of 100 μL or less
Silica Monolith packing material
New separation media that are neither particulate nor membrane
Silica monoliths are integral silica gels with uniform continuous pores synthesized from ethyl silicate. They have high liquid permeability and large surface area possessing a structure with through-pores and mesopores on the framework surface. This state-of-the-art media is becoming popular globally for its characteristics: high recovery and high performance of adsorption and desorption.
Advantages to conventional particle type packing
- Disk-shaped silica monoliths do not use frits to hold particle media
- The massive surface area of monolithic material makes it possible makes it possible to retain samples in the cartridge and completely elute small samples during processing allowing for much smaller volumes of sample needed.
- Despite high liquid permeability, silica monoliths are still suitable for rapid elution without the loss of high recovery with fast sample diffusion and quick separation.
How to Operate
Steps in Centrifugation for Spin Columns
Whole process for sample treatment can be done within 10 minutes.
Performance in analysis of trace analytes
Due to its high permeability, MonoSpin series enables collection of trace analytes without the need for dilution.
S type Column
L Type Column
