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InertSustain AX-C18

Brand: InertSustain
InertSustain AX-C18 is a mix-mode column bonded with C18 groups and tertiary amino groups. It provides hydrophobic interaction and electrostatic interaction by Anion Exchange group works. By controlling the amount of Anion Exchange group, acidic compounds can be eluted even in the buffer concentration range used with LC/MS.

Overview

Retention behavior that combines both reversed phase and anion exchange

 

InertSustain AX-C18 Anion Exchange Column has increased retention of acidic compounds/decreased retention of basic compounds compared to typical C18 columns.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of nucleobases and monophosphates graph

Compounds:

1. Cytosine (basic)  2. Uracil (neutral)  3. CMP (acidic)  4. UMP (acidic)  5. Adenine (basic)  6. AMP (acidic)

InertSustain AX-C18 Anion Exchange Column HPLC Columns compounds 1
InertSustain AX-C18 Anion Exchange Column HPLC Columns compounds 2
 

Excellent retention for acidic compounds

 

No ion pair reagent in the eluent means highly polar acidic compounds are retained.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of sugar-nucleotides

Strongly retains highly polar acidic compounds which normally cannot be retained by C18 columns for analysis of highly polar compounds!

InertSustain AX-C18 Anion Exchange Column strongly retains acidic compounds even if the salt concentration of the eluent is low. Also compatible with MS analysis.

InertSustain AX-C18 Anion Exchange Column HPLC Columns Analysis of monophosphoric acid

Strongly retains highly polar acidic compounds even when salt concentration is as low as 10 mM! Sharp elution peaks!

Can be analyzed at low salt concentration. Compatibility with MS!

 

Excellent reproducibility and quality

 

Reproducibility issues with mixed mode are solved by GL Sciences' original packing and manufacturing technology.

InertSustain AX-C18 Anion Exchange Column HPLC Columns lot to lot variation graph
InertSustain AX-C18 Anion Exchange Column HPLC Columns lot to lot variation in acidic compounds graph

Excellent reproducibility of retention time in mixed‐mode columns where reproducibility is difficult to control!

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250 mm
3.0 mm
5 µm
$740.00
150 mm
3.0 mm
5 µm
$690.00
100 mm
2.1 mm
5 µm
$640.00
250 mm
2.1 mm
5 µm
$740.00
50 mm
4.0 mm
5 µm
$580.00
50 mm
2.1 mm
5 µm
$580.00
100 mm
3.0 mm
5 µm
$640.00
100 mm
4.0 mm
5 µm
$640.00
150 mm
2.1 mm
5 µm
$690.00
50 mm
3.0 mm
5 µm
$580.00

Applications

There are application notes for InertSustain AX-C18.

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