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Inertsil Hybrid-C18 — High-pH C18 HPLC Column | GL Sciences USA
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pH 11.5 durability data Published data
Consistent Theoretical Plate Count at High pH
pH 11.5 durability comparison chart

Source: GL Sciences
See full chart below

Inertsil Hybrid-C18

Consistent Performance
Across a Wide pH Range

Inertsil Hybrid-C18 provides reliable performance across a wide pH range (pH 1–12), giving method developers the flexibility to optimize retention and selectivity. It delivers excellent peak shape and durability while offering a cost-effective alternative to Waters BEH or XBridge columns.

Reliable performance across pH 1–12 for flexible method development
Excellent peak shape for basic, acidic, and metal-coordinating compounds
High durability and consistent reproducibility across batches
Maintains reliable retention behavior in highly aqueous mobile phases, supporting the analysis of polar and early-eluting compounds.
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Equivalent To:
ACQUITY UPLC BEH C18 ACQUITY UPLC BEH130 C18 XBridge C18 XBridge Shield RP18 XBridge Peptide BEH C18 130A XBridge Oligo BEH C18 XTerra MS C18
→
Alkali-Resistant Hybrid Silica Technology

Superior Durability Under High pH Conditions (pH 11.5)

Mobile phase pH can significantly impact column stability and performance. Inertsil Hybrid-C18 is designed to maintain consistent performance across a wide pH range (pH 1–12), enabling reliable separations under both acidic and basic conditions.

Inertsil Hybrid-C18 maintains theoretical plate count through the full test, while commonly compared columns decline earlier or fall sharply later in the test.

Consistent Theoretical Plate Count at High pH

Consistent Theoretical Plate Count at High pH — Inertsil Hybrid-C18 durability test at pH 11.5 over 250 hours

Full test conditions are available in the brochure.

Excellent batch-to-batch reproducibility

Three production lots show virtually identical retention and peak shape, helping support consistent long-term method performance.

Excellent batch-to-batch reproducibility — three production lots with virtually identical retention and peak shape
100% QC tested
Certificate of Analysis (CoA) provided to ensure consistent performance between lots.

Superior Performance Across Challenging Analytes

Application data demonstrates strong peak shape across basic, acidic, and metal-coordinating compounds.

Chromatogram comparison — Inertsil Hybrid-C18 peak shape across basic, acidic, and metal-coordinating compounds
Inertsil Hybrid-C18 Brochure
Download the Inertsil Hybrid-C18 Brochure Durability data, specifications, and application examples Download Brochure
Method Development Flexibility
One Column, Infinite Possibilities

These chromatograms demonstrate how changes in mobile phase pH influence the selectivity and resolution of analytes. Inertsil Hybrid-C18 allows stable performance across this wide pH range without loss of efficiency and reproducibility.

pH selectivity chart — Inertsil Hybrid-C18 retention behavior across pH 1–12
No. Compound
1Epinastine
2Cetirizine
3Fexofenadine
4Chlorpheniramine
5Ketotifen
6Hydroxyzine
7Triprolidine
8Azelastine
9Loratadine
10Homochlorcyclizine
11Clemastine
Equivalent To:
Comparable Performance. Competitive Price.

Inertsil Hybrid-C18 provides performance comparable to major hybrid silica columns while offering a more cost-effective option for laboratories.

Waters
ACQUITY UPLC BEH C18, ACQUITY UPLC BEH130 C18
XBridge C18, XBridge Shield RP18, XBridge Peptide BEH C18 130A, XBridge Oligo BEH C18
XTerra MS C18
Agilent
ZORBAX Extend C18
Phenomenex
Gemini C18, Gemini-NX C18
YMC
YMC-Triart C18
Inertsil Hybrid-C18
Specifications
pH Range: 1–12
Surface Area: 185 m²/g
Particle Sizes: 1.7 μm, 3.5 μm, 5 μm
Max Temperature: 80°C (pH 1–10), 60°C (pH 1–12)
Pore Size: 165 Å
USP Classification: L1
Carbon Loading: 15%
Hardware Options: Stainless steel (SS) · biocompatible PEEK
Column Hardware Options
Wide Range of Dimensions and Hardware Options

Hardware is available in two choices: stainless steel (SS) and biocompatible PEEK, making it suitable for everything from routine analyses to LC/MS, LC/MS/MS, and sensitive biological applications.

PEEK and stainless steel hardware options
Customized Dimensions Available on Request

In addition to our standard lineup, custom column dimensions and configurations can be made to order, including preparative-scale dimensions and specialty hardware options.

Customized column dimensions
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Or call: 1-310-984-1946
Inertsil Hybrid-C18 Brochure
Download the Inertsil Hybrid-C18 Brochure
Durability data, specifications, and application examples
Download Brochure