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Inertsil 2 micron UHPLC Columns

Brand: Inertsil

Overview

GL Sciences' UltraFast Kits transform any HPLC into a UHPLC grade instrument.


Transforming “standard” gradient HPLC systems into extremely fast gradient systems is readily achievable with proper application of chromatographic principles and using advanced HPLC columns and a new temperature control system. You don't have to change your LC to go fast!

The quest to pursue “ultra” fast LC separations is gaining momentum as the result of recent product introductions to address this need. These new products claim to deliver the same separations while using 5–10 fold less time. What may be lesser known is that routine performance of “ultra” fast separations can be achieved using many of the ordinary main stream HPLCs that one may already have available. Achieving this level of performance is simply a matter of knowledge of the separation principles involved and their practical implementation. A key principle involved is balancing flow with molecular velocity in all other directions. The implementation of the LC set up involves volume control in the system and its eluted peaks. The implementation of the LC analyses involves making good choices in stationary and mobile phases as well as full proper control of critical system parameters such as mobile phase temperature (entering column). Application of the above concepts allows routine analyses of > 500 samples per day per LC (or LC–MS) operating at 7 mm/s
(5 mL/min, 5X “normal”), clearing ~ 9 column volumes per min (10X “normal”) and delivering peaks < 1 second wide. The direct result of this added capacity is the straightforward and scalable positive impact on turnaround times of existing analyses while conserving resolution (quality).

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Applications

There are application notes for Inertsil 2 micron UHPLC Columns.

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