Phos-TiO Phosphopeptide Enrichment

GL Sciences has been manufacturing extremely pure Titanium Dioxide microparticles for many years, and has recently introduced the most efficient producs available for phosphopeptide enrichment from proteolytic digests or other complex matrices.

GL Sciences' Phos-TiO Kits are the latest addition to our proteomics portfolio. These kits contain centrifuge spin columns, conditioning and washing buffers, along with waste and collection tubes for both individual column processing and 96-well plate bulk processing. The titanium dioxide particles contained in the spin columns (available in 1mg/10uL and 3mg/200uL sizes) is specially treated to maximize selectivity for phosphorylated species, and the conditioning and washing buffers contain components to displace the few non-phosphorylated compounds which might originally adhere to the media.

This same specialized media is now available in 50mg/3mL and 100mg/3mL spin columns for larger scale/volume purifications.

Desalting Phosphopeptide-Enriched Samples

GL-Tip™ SDB and GL-Tip™ GC Desalting of TiO2-Enriched Samples Prior to LC/MS Phosphopeptides isolated using TiO2-based medias are typically desalted prior to analysis by LC/MS, typically using a C18 (hydrophobic) micropipette tip.. GL Sciences’ SDB (styrene divinylbenzene) and GC (graphite carbon) centrifugeoperated micropipette GL-TipsTM retain more hydrophobic and hydrophilic peptides, respectively, than C18-based tips.

Highly Retentive for Hydrophobic and Hydrophilic Peptides

GL-TipTM SDB are more hydrophobic than C18 medias and allow retention of a wider range of phosophopeptides with high yield, allowing more accurate analysis of phosphopeptide species present in the sample. GL-TipTM GC retain many more hydrophilic phosphopeptides than does C18; by using a combination of GL-TipTM SDB and GC, almost all peptide samples can be de-salted without sample losses due to lack of retention. Easy to Operate Phosphopeptide-enriched samples are easily loaded, washed, and eluted using the same centrifuge-based technique used with Phos-TiO tips.

MonoTip-TiO Phosphopeptide Enrichment Pipette Tips

MonoTip TiO pipette-based columns contain GL Sciences’ silica monolith completely coated with nanoparticles of titanium dioxide.

MonoTip TiO was GL Sciences’ first TiO2 based product for low-pressure phophopeptide enrichment, and offers the convenience of rapid enrichment and are widely used today; however, Phos- TiO centrifuge-spin columns contain an advanced form of TiO2 which provides superior selectivity and capacity and allows many individual samples to be processed simultaneously. (For new customers, we recommend evaluating Phos-TiO products as a first choice.)

MonoTip C18 pipette tips (compare to Waters' ZipTip) are available for desalting and micro-SPE / Sample Enrichment of non-phosphorylated peptides and proteins.

MonoTip are easy to use - samples are easily loaded, washed, and eluting using a pipette (single or multiple port). MonoTip TiO pipette tips are used for sample volumes of 50 - 200 μL, with approximately 5ug of phosphopeptide bound per tip. Owing to the open prorosity of the monolith contained in these tips, samples can be eluted with small volumes, thereby substantially concentrating phosphopeptides