Inertsil HILIC

What is HILIC mode? Hydrophilic Interaction Chromatography Analysis of highly polar and basic compounds has been problematic since the beginning of HPLC. Some polar compounds are so difficult to analyze that they require high aqueous buffer content, even on the most inert reverse phase HPLC columns, resulting in imperfect peak shape and impaired MS sensitivity.  Inertsil HILIC has proven particularly effective in detecting melamine in food products.

HILIC was developed as an alternative to reverse phase chromatography for polar compounds, and has also shown great promise in SFC. The elution order in HILIC mode is typically the opposite of what it might be in reverse phase mode. In HILIC, the HIGHER the organic concentration, the GREATER the retention of more polar compounds.  Advantages of HILIC mode High organic content mobile phases result in low operating back pressure and ability to run at high flow rates A high organic solvent concentration of the mobile phase will lead to a high sensitivity LC/MS analysis.

Reduced Ion suppression effect in LC/MS analyses. More precise quantitation of polar compounds owing to improved peak shape and the rapid elution of hydrophobic sample components.

Add To CartOrder No.Japan PartDescriptionLengthDiameterUSA PriceQuantity
        
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1022C050W050 5020-15025Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 50 x 0.05mm500.05640
1022C150W050 5020-15075Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 150 x 0.05mm1500.05680
1022C250W050 5020-15125Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 250 x 0.05mm2500.05720
1022C050W075 5020-15175Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 50 x 0.075mm500.075640
1022C150W075 5020-15225Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 150 x 0.075mm1500.075680
1022C250W075 5020-15275Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 250 x 0.075mm2500.075720
1022C050W100 5020-15325Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 50 x 0.1mm500.1640
1022C150W100 5020-15375Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 150 x 0.1mm1500.1680
1022C250W100 5020-15425Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 250 x 0.1mm2500.1720
1022C050W200 5020-15475Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 50 x 0.2mm500.2640
1022C150W200 5020-15525Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 150 x 0.2mm1500.2680
1022C250W200 5020-15575Inertsil HILIC, 3 micron, Capillary EX-Nano Column, 250 x 0.2mm2500.2720
0001 5020-01855Lcap Splitter for EX-Nano  350
0002 5020-01881Capillary EX-nano Connection Kit  130
1022C050W300 5020-11525Inertsil HILIC, 3 micron, Capillary-EX Column, 50 x 0.3mm500.3640
1022C150W300 5020-11575Inertsil HILIC, 3 micron, Capillary-EX Column, 150 x 0.3mm1500.3680
1022C050W500 5020-11625Inertsil HILIC, 3 micron, Capillary-EX Column, 50 x 0.5mm500.5640
1022C150W500 5020-11675Inertsil HILIC, 3 micron, Capillary-EX Column, 150 x 0.5mm1500.5680
1022C050W700 5020-11725Inertsil HILIC, 3 micron, Capillary-EX Column, 50 x 0.7mm500.7640
1022C150W700 5020-11775Inertsil HILIC, 3 micron, Capillary-EX Column, 150 x 0.7mm1500.7680
1022G020W300 5020-11825Inertsil HILIC, 3 micron, Capillary-EX Guard Column, 2 x 0.3mm20.3180
0003 5020-01880Cap-EX connection Kit  90
1022L033W010 5020-86731Inertsil HILIC, 3 micron, 33 x 1.0mm331400
1022L050W010 5020-86732Inertsil HILIC, 3 micron, 50 x 1.0mm501400
1022L075W010 5020-86733Inertsil HILIC, 3 micron, 75 x 1.0mm751450
Add To CartOrder No.Japan PartDescriptionLengthDiameterUSA PriceQuantity
        
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1021C050W050 5020-15024Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 50 x 0.05mm500.05640
1021C150W050 5020-15074Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 150 x 0.05mm1500.05680
1021C250W050 5020-15124Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 250 x 0.05mm2500.05720
1021C050W075 5020-15174Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 50 x 0.075mm500.075640
1021C150W075 5020-15224Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 150 x 0.075mm1500.075680
1021C250W075 5020-15274Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 250 x 0.075mm2500.075720
1021C050W100 5020-15324Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 50 x 0.1mm500.1640
1021C150W100 5020-15374Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 150 x 0.1mm1500.1680
1021C250W100 5020-15424Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 250 x 0.1mm2500.1720
1021C050W200 5020-15474Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 50 x 0.2mm500.2640
1021C150W200 5020-15524Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 150 x 0.2mm1500.2680
1021C250W200 5020-15574Inertsil HILIC, 5 micron, Capillary EX-Nano Column, 250 x 0.2mm2500.2720
0001 5020-01855Lcap Splitter for EX-Nano  350
0002 5020-01881Capillary EX-nano Connection Kit  130
1021C050W300 5020-11524Inertsil HILIC, 5 micron, Capillary-EX Column, 50 x 0.3mm500.3640
1021C150W300 5020-11574Inertsil HILIC, 5 micron, Capillary-EX Column, 150 x 0.3mm1500.3680
1021C050W500 5020-11624Inertsil HILIC, 5 micron, Capillary-EX Column, 50 x 0.5mm500.5640
1021C150W500 5020-11674Inertsil HILIC, 5 micron, Capillary-EX Column, 150 x 0.5mm1500.5680
1021C050W700 5020-11724Inertsil HILIC, 5 micron, Capillary-EX Column, 50 x 0.7mm500.7640
1021C150W700 5020-11774Inertsil HILIC, 5 micron, Capillary-EX Column, 150 x 0.7mm1500.7680
1021G020W300 5020-11824Inertsil HILIC, 5 micron, Capillary-EX Guard Column, 2 x 0.3mm20.3180
0003 5020-01880Cap-EX connection Kit  90
1021L033W010 5020-86711Inertsil HILIC, 5 micron, 33 x 1.0mm331380
1021L050W010 5020-86712Inertsil HILIC, 5 micron, 50 x 1.0mm501380
1021L075W010 5020-86713Inertsil HILIC, 5 micron, 75 x 1.0mm751430
Add To CartOrder No.Japan PartDescriptionLengthDiameterUSA PriceQuantity
        
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1025L050W060 5020-Hi-050-060Inertsil HILIC, Prep, 50 x 6.0mm506420
1025L050W076 5020-Hi-050-076Inertsil HILIC, Prep, 50 x 7.6mm507.6560
1025L050W100 5020-Hi-050-100Inertsil HILIC, Prep, 50 x 10.0mm5010745
1025L050W200 5020-Hi-050-200Inertsil HILIC, Prep, 50 x 20.0mm50201145
1025L050W300 5020-Hi-050-300Inertsil HILIC, Prep, 50 x 30.0mm50302320
1025L100W060 5020-Hi-100-060Inertsil HILIC, Prep, 100 x 6.0mm1006425
1025L150W060 5020-Hi-150-060Inertsil HILIC, Prep, 150 x 6.0mm1506445
1025L250W060 5020-Hi-250-060Inertsil HILIC, Prep, 250 x 6.0mm2506520
1025L250W076 5020-Hi-250-076Inertsil HILIC, Prep, 250 x 7.6mm2507.6735
1025L250W100 5020-Hi-250-100Inertsil HILIC, Prep, 250 x 10.0mm250101175
1025L250W200 5020-Hi-250-200Inertsil HILIC, Prep, 250 x 20.0mm250202910
1025L250W300 5020-Hi-250-300Inertsil HILIC, Prep, 250 x 30.0mm250305225
1025L250W500 5020-Hi-250-500Inertsil HILIC, Prep, 250 x 50.0mm2505011150
1025G050W060 5020-Hi-050-060-GInertsil HILIC, Prep Guard, 50 x 6.0mm506285
1025G050W076 5020-Hi-050-076-GInertsil HILIC, Prep Guard, 50 x 7.6mm507.6370
1025G050W100 5020-Hi-050-100-GInertsil HILIC, Prep Guard, 50 x 10.0mm5010685
1025G050W200 5020-Hi-050-200-GInertsil HILIC, Prep Guard, 50 x 20.0mm50201170
1025G050W300 5020-Hi-050-300-GInertsil HILIC, Prep Guard, 50 x 30.0mm50302285
1025G075W500 5020-Hi-075-500Inertsil HILIC, Prep Guard, 75 x 50.0mm75504270
0004 5020-08500Guard Cartridge Holder, for 10mm Cartridges  150
0005 5020-08550Guard Cartridge Holder, for 20mm Cartridges  150
0008 5020-06920Prep Guard Cartridge Holder  230