Analysis of Drugs in Plasma

Analysis of drugs in plasma has traditionally required sample preparation to remove plasma proteins before HPLC analysis to avoid precipitation of plasma proteins and subsequent column clogging.

Bioptic AV columns allow the inner hydrophobic surface of the silica to be shielded by a permanently bonded hydrophilic avidin layer.  Large molecules like plasma proteins are excluded from interacting with the hydrophobic inner surface by the avidin layer, and elute by size-exclusion mechanisms in the void volume.  Smaller compounds, such as drugs and drug metabolites, pass through the avidin layer and interact with the hydrophobic inner layer by a reverse-phase mechanism. Bioptic AV-1 contains non-denatured avidin and a chiral center, allowing separation of chiral drugs and their metabolites.  Bioptic AV-2 contains a denatured form of avidin and lacks the chiral separating ability of AV-1, but offers different selectivity.

GL Sciences has compiled a collection of chromatographic applications data on Bioptic AV-1 and AV-2 and their other phases which can assist you in method development.  These can be accessed under the "chromatograms" link on the main menu bar above, by searching for a particular compound using our "search" link above, or by clicking Bioptic columns are made with a 5 micron spherical silica, and the columns withstand pressures up to about 2250 PSI.  AV-1 has a limit of about 20% on the organic content of the eluent, which there is no such limitation on AV-2. Recommended pH range is 2 to 7.5, while pH ranges where hydrophobic interactions occur are between 2-3.5 and between 6 and 7.5.

Add To CartOrder No.Japan PartDescriptionLengthDiameterUSA PriceQuantity
        
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1340L150W015 5020-18153BIOPTIC AV-1, Plasma Direct Inject, 150 x 1.5mm1501.51440
1340L150W021 5020-02521BIOPTIC AV-1, Plasma Direct Inject, 150 x 1.5mm1502.11760
1340L150W030 5020-02522BIOPTIC AV-1, Plasma Direct Inject, 150 x 1.5mm15031760
1340L150W040 5020-02523BIOPTIC AV-1, Plasma Direct Inject, 150 x 1.5mm15041760
1340L150W046 5020-02524BIOPTIC AV-1, Plasma Direct Inject, 150 x 1.5mm1504.61760
1340G050W046 5020-08320BIOPTIC AV-1, Plasma Direct Inject, Guard, 50 x 4.6mm504.6760
0004 5020-08500Guard Cartridge Holder for 10mm length cartridges, reusable  150
1340P010W010 5020-19243BIOPTIC AV-1, 2 Guard Cartridges, 10 x 1.0mm101520
1340P010W015 5020-19343BIOPTIC AV-1, 2 Guard Cartridges, 10 x 1.5mm101.5520
1340P010W030 5020-19143BIOPTIC AV-1, 2 Guard Cartridges, 10 x 3.0mm103520
1340P010W040 5020-19043BIOPTIC AV-1, 2 Guard Cartridges, 10 x 4.0mm104520
1340P1KIT010 5020-19293BIOPTIC AV-1, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 1.0mm101640
1340P1KIT015 5020-19393BIOPTIC AV-1, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 1.5mm101.5640
1340P1KIT030 5020-19193BIOPTIC AV-1, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 3.0mm103640
1340P1KIT040 5020-19093BIOPTIC AV-1, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 4.0mm104640
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1342L150W015 5020-18154BIOPTIC AV-2, Plasma Direct Inject, 150 x 1.5mm1501.51360
1342L150W021 5020-02531BIOPTIC AV-2, Plasma Direct Inject, 150 x 1.5mm1502.11240
1342L150W030 5020-02532BIOPTIC AV-2, Plasma Direct Inject, 150 x 1.5mm15031240
1342L150W040 5020-02533BIOPTIC AV-2, Plasma Direct Inject, 150 x 1.5mm15041240
1342L150W046 5020-02534BIOPTIC AV-2, Plasma Direct Inject, 150 x 1.5mm1504.61240
1342G050W046 5020-08321BIOPTIC AV-2, Plasma Direct Inject, Guard, 50 x 4.6mm504.6420
0004 5020-08500Guard Cartridge Holder for 10mm length cartridges, reusable  150
1342P010W010 5020-19244BIOPTIC AV-2, 2 Guard Cartridges, 10 x 1.0mm101300
1342P010W015 5020-19344BIOPTIC AV-2, 2 Guard Cartridges, 10 x 1.5mm101.5300
1342P010W030 5020-08622BIOPTIC AV-2, 2 Guard Cartridges, 10 x 3.0mm103300
1342P010W040 5020-08620BIOPTIC AV-2, 2 Guard Cartridges, 10 x 4.0mm104300
1342P1KIT010 5020-19294BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 1.0mm101440
1342P1KIT015 5020-19394BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 1.5mm101.5440
1342P1KIT030 5020-08623BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 3.0mm103440
1342P1KIT040 5020-08621BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 10 x 4.0mm104440
0005 5020-08550Guard Cartridge Holder for 20mm length cartridges, reusable  150
1342P020W030 5020-08626BIOPTIC AV-2, 2 Guard Cartridges, 20 x 3.0mm203420
1342P020W040 5020-08624BIOPTIC AV-2, 2 Guard Cartridges, 20 x 4.0mm204420
1342P2KIT030 5020-08627BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 20 x 3.0mm203580
1342P2KIT040 5020-08625BIOPTIC AV-2, Guard Kit, 1 Holder plus 2 Guard Cartridges, 20 x 4.0mm204580